| Definition | Oceanobacillus iheyensis HTE831, complete genome. |
|---|---|
| Accession | NC_004193 |
| Length | 3,630,528 |
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The map label for this gene is dkgB [H]
Identifier: 23097844
GI number: 23097844
Start: 413306
End: 414148
Strand: Reverse
Name: dkgB [H]
Synonym: OB0389
Alternate gene names: 23097844
Gene position: 414148-413306 (Counterclockwise)
Preceding gene: 23097848
Following gene: 23097836
Centisome position: 11.41
GC content: 37.72
Gene sequence:
>843_bases ATGGTAAATCAAATCCCAAACGTTACACTACATGATGGACTAACCATTCCGAAAGTAGGTTTTGGAACCTATCGTTTAAA TGGTAACGATGGTGTACAAGCCATTAATAATGCCATTGATCATGGGTATCGTTTAATCGACACTGCCTATAACTATGAGA ATGAAGGAACTGTTGGTGAGGCGGTTAGAAGAAGTTCCACACCTCGAGAACAGCTGCTTATTACTTCTAAACTACCAGGT CGATATCATCACTATGATAAAGCAGTTACTACGATCCAAGAATCTTTATATCGAGCAAACCTTGATTATTATGACTTATA TCTTATTCACTGGCCAAATCCAATCCAAGATATATATGTAGAAGCATGGCAAGCTTTAATTGAGGCCAAACGCAGGGGAT ATATCCGTTCTATCGGAGTATGTAATTTCTTACCAGAACACTTAGAGCGACTTAAACAGGAAACTGGGGAATTACCAACG ATAAACCAGATTGAATTACATCCTTTCTTTAACCAAGAAGACCAGCGTAAATGGCATCAAGAACATCAAATCGCTACCGA ATCTTGGAGCCCATTGGCACGTACAAATGATGTATTAGACCATCCTGTTTTACAAAAGCTCGCTTCGAACCATAACCGAA CGGTATCTCAAATAATTTTACGTTGGCATTATCAACTTGATGCGATTGCAATACCTAAATCTGCAACGCCTTCAAGACAG CTCGAAAACATTTCTATTTTTGATTTTGAATTATCTGATGAAGAAATGTCTATTATTAATCAGCTATCAAAAGTGGATGG TCGTATGAAAGATCAAGACCCAGCAGTTTATGAAGAGTTTTAA
Upstream 100 bases:
>100_bases GCTTCACCCTTTTATAATCAAATTCACTTTTATGCAAATGGTTTGAATTTTTAATAATGCAAGCGTAATCTATAGTTTAT ATATGGAAGGAGAGGATTAC
Downstream 100 bases:
>100_bases GATATAACCATTTTTGGTTAAAATGCCTTTTATCTAGAAAGGCATGAATTCGTAAATTAGACTATTTCTCTTACATCATA GGCGTAGTCACAAAACCCCC
Product: 2,5-diketo-D-gluconate reductase
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 280; Mature: 280
Protein sequence:
>280_residues MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGEAVRRSSTPREQLLITSKLPG RYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYVEAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPT INQIELHPFFNQEDQRKWHQEHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF
Sequences:
>Translated_280_residues MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGEAVRRSSTPREQLLITSKLPG RYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYVEAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPT INQIELHPFFNQEDQRKWHQEHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF >Mature_280_residues MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGEAVRRSSTPREQLLITSKLPG RYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYVEAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPT INQIELHPFFNQEDQRKWHQEHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 25DKGR-B has higher catalytic efficiency than 25DKGR-A [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=34.3859649122807, Blast_Score=170, Evalue=2e-42, Organism=Homo sapiens, GI24497577, Length=308, Percent_Identity=32.7922077922078, Blast_Score=167, Evalue=1e-41, Organism=Homo sapiens, GI5174391, Length=308, Percent_Identity=32.7922077922078, Blast_Score=167, Evalue=1e-41, Organism=Homo sapiens, GI93277124, Length=295, Percent_Identity=32.5423728813559, Blast_Score=158, Evalue=7e-39, Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=33.8983050847458, Blast_Score=152, Evalue=3e-37, Organism=Homo sapiens, GI291291012, Length=260, Percent_Identity=34.6153846153846, Blast_Score=152, Evalue=4e-37, Organism=Homo sapiens, GI4502049, Length=283, Percent_Identity=34.2756183745583, Blast_Score=147, Evalue=7e-36, Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=32.6530612244898, Blast_Score=146, Evalue=2e-35, Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=32.6530612244898, Blast_Score=145, Evalue=3e-35, Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=34.0579710144928, Blast_Score=145, Evalue=4e-35, Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=32.6530612244898, Blast_Score=145, Evalue=5e-35, Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=32.312925170068, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=32.312925170068, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=31.5254237288136, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=32.129963898917, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=32.0143884892086, Blast_Score=120, Evalue=9e-28, Organism=Homo sapiens, GI310109924, Length=149, Percent_Identity=32.2147651006711, Blast_Score=81, Evalue=1e-15, Organism=Homo sapiens, GI310109926, Length=132, Percent_Identity=37.1212121212121, Blast_Score=78, Evalue=8e-15, Organism=Homo sapiens, GI207028673, Length=125, Percent_Identity=37.6, Blast_Score=77, Evalue=2e-14, Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=44.4029850746269, Blast_Score=234, Evalue=4e-63, Organism=Escherichia coli, GI1786400, Length=252, Percent_Identity=36.9047619047619, Blast_Score=157, Evalue=8e-40, Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=30.4832713754647, Blast_Score=109, Evalue=2e-25, Organism=Caenorhabditis elegans, GI17537077, Length=305, Percent_Identity=34.7540983606557, Blast_Score=163, Evalue=1e-40, Organism=Caenorhabditis elegans, GI17537075, Length=302, Percent_Identity=34.7682119205298, Blast_Score=162, Evalue=1e-40, Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=35.1851851851852, Blast_Score=162, Evalue=1e-40, Organism=Caenorhabditis elegans, GI71998625, Length=274, Percent_Identity=34.3065693430657, Blast_Score=155, Evalue=2e-38, Organism=Caenorhabditis elegans, GI17564128, Length=316, Percent_Identity=31.0126582278481, Blast_Score=148, Evalue=3e-36, Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=34.4444444444444, Blast_Score=146, Evalue=9e-36, Organism=Caenorhabditis elegans, GI17537079, Length=299, Percent_Identity=30.7692307692308, Blast_Score=142, Evalue=2e-34, Organism=Caenorhabditis elegans, GI17552492, Length=257, Percent_Identity=31.1284046692607, Blast_Score=139, Evalue=2e-33, Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=31.5018315018315, Blast_Score=134, Evalue=5e-32, Organism=Caenorhabditis elegans, GI17566692, Length=291, Percent_Identity=29.553264604811, Blast_Score=134, Evalue=5e-32, Organism=Caenorhabditis elegans, GI17550248, Length=309, Percent_Identity=29.126213592233, Blast_Score=125, Evalue=3e-29, Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=29.1970802919708, Blast_Score=106, Evalue=1e-23, Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=32.5342465753425, Blast_Score=163, Evalue=2e-41, Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=31.6666666666667, Blast_Score=156, Evalue=4e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=314, Percent_Identity=31.2101910828025, Blast_Score=137, Evalue=3e-33, Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=30, Blast_Score=135, Evalue=1e-32, Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=32.312925170068, Blast_Score=126, Evalue=4e-30, Organism=Saccharomyces cerevisiae, GI6320079, Length=280, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=5e-24, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=32.9824561403509, Blast_Score=163, Evalue=1e-40, Organism=Drosophila melanogaster, GI24657054, Length=281, Percent_Identity=34.8754448398576, Blast_Score=162, Evalue=2e-40, Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=34.1379310344828, Blast_Score=159, Evalue=1e-39, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=32.5174825174825, Blast_Score=157, Evalue=6e-39, Organism=Drosophila melanogaster, GI24644950, Length=309, Percent_Identity=31.3915857605178, Blast_Score=151, Evalue=5e-37, Organism=Drosophila melanogaster, GI24662789, Length=304, Percent_Identity=31.25, Blast_Score=145, Evalue=2e-35, Organism=Drosophila melanogaster, GI221378297, Length=329, Percent_Identity=29.7872340425532, Blast_Score=144, Evalue=6e-35, Organism=Drosophila melanogaster, GI45553081, Length=280, Percent_Identity=32.1428571428571, Blast_Score=139, Evalue=2e-33, Organism=Drosophila melanogaster, GI24662781, Length=281, Percent_Identity=33.0960854092527, Blast_Score=134, Evalue=7e-32, Organism=Drosophila melanogaster, GI24662785, Length=280, Percent_Identity=33.5714285714286, Blast_Score=133, Evalue=1e-31, Organism=Drosophila melanogaster, GI281366140, Length=244, Percent_Identity=31.5573770491803, Blast_Score=115, Evalue=3e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 32479; Mature: 32479
Theoretical pI: Translated: 5.73; Mature: 5.73
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.4 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGE CCCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEHHHHHCCCCCCCHHH AVRRSSTPREQLLITSKLPGRYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYV HHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHH EAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPTINQIELHPFFNQEDQRKWHQ HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH EHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCCCHH LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCC >Mature Secondary Structure MVNQIPNVTLHDGLTIPKVGFGTYRLNGNDGVQAINNAIDHGYRLIDTAYNYENEGTVGE CCCCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCEEHHHHHCCCCCCCHHH AVRRSSTPREQLLITSKLPGRYHHYDKAVTTIQESLYRANLDYYDLYLIHWPNPIQDIYV HHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHH EAWQALIEAKRRGYIRSIGVCNFLPEHLERLKQETGELPTINQIELHPFFNQEDQRKWHQ HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH EHQIATESWSPLARTNDVLDHPVLQKLASNHNRTVSQIILRWHYQLDAIAIPKSATPSRQ HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCCCHH LENISIFDFELSDEEMSIINQLSKVDGRMKDQDPAVYEEF HCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA