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The map label for this gene is fabI [H]

Identifier: 23097678

GI number: 23097678

Start: 243242

End: 244018

Strand: Reverse

Name: fabI [H]

Synonym: OB0223

Alternate gene names: 23097678

Gene position: 244018-243242 (Counterclockwise)

Preceding gene: 23097696

Following gene: 23097677

Centisome position: 6.72

GC content: 38.48

Gene sequence:

>777_bases
ATGACGAAACTATTAGAAGGTAAAAATATTGTAGTTATGGGTGTTGCAAATGAACGTAGTATCGCTTGGGGAATAACGAA
ATCTTTACACAATGCTGGCGCTAATTTAATTTTTACAAATAGACAAGAACGTTCTTATCAAAAACTAGTGAAACTATTAG
ATAAACATGAAATACAAGCTAAATTAATTGTATCTTGCGATGTAGCTGACGATGAAAGTATCCAACAAGCTTTCAATGAA
ATCGGAGAAAAAGTAGGCGTTATTCACGGTGTTGTTCATTCTGTTGCGTTCGCAAACCGAGATGAACTACAAGGAGAATA
TGCAAATACATCAAGAGATGGGTTCTTACTTGCACAAGAAATCAGTGCGTACTCTTTAGTCGCTGTTACAAAAGCTGCTA
AAGAACTAATGACAGAAGGTGGAGGAATCGTAACACAGACTTATCTAGGGGCAGAACGAGTTATTCCTGGATATAATGTC
ATGGGAGTTGCAAAAGCATCTTTAGAAGCAAGTGTCCGCTACCTAGCTGAAGACATGGGTAAATACGGTATCCGAGTTAA
CGCAGTTTCTGCCGGACCAATTCGTACACTTTCTGCAAAAGGAGTCTCTAACTTTAATGAAAAAGCAAATGTTATAGTAG
AAAAAGCTCCACTACGTAGAAATGTAGATCAAGATGAAGTTGGCGACGCAACGCTATTTTTAGTTAGTGAAATGGCTCGT
GGGATCACTGGGGAAGTTCTTCATGTGGATTCAGGCTTTCATATCATAGGTGGATAG

Upstream 100 bases:

>100_bases
TTCCTTACCAACCTCAAATTTATCGTTTTTATGTTTTGGTTTCATTACCAGGTACTAATAAGTTATAATAAATAAATAAT
GAATCATTTGGAGGTAAATT

Downstream 100 bases:

>100_bases
TTCAGCAGAAAGTTGTAAAATCAAATTTTAAAAAAACACTTTAGACATATATAGAACGCATATCCAACGGATGGCGAGTA
GGGCCCCAGATGTTCATACA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTKLLEGKNIVVMGVANERSIAWGITKSLHNAGANLIFTNRQERSYQKLVKLLDKHEIQAKLIVSCDVADDESIQQAFNE
IGEKVGVIHGVVHSVAFANRDELQGEYANTSRDGFLLAQEISAYSLVAVTKAAKELMTEGGGIVTQTYLGAERVIPGYNV
MGVAKASLEASVRYLAEDMGKYGIRVNAVSAGPIRTLSAKGVSNFNEKANVIVEKAPLRRNVDQDEVGDATLFLVSEMAR
GITGEVLHVDSGFHIIGG

Sequences:

>Translated_258_residues
MTKLLEGKNIVVMGVANERSIAWGITKSLHNAGANLIFTNRQERSYQKLVKLLDKHEIQAKLIVSCDVADDESIQQAFNE
IGEKVGVIHGVVHSVAFANRDELQGEYANTSRDGFLLAQEISAYSLVAVTKAAKELMTEGGGIVTQTYLGAERVIPGYNV
MGVAKASLEASVRYLAEDMGKYGIRVNAVSAGPIRTLSAKGVSNFNEKANVIVEKAPLRRNVDQDEVGDATLFLVSEMAR
GITGEVLHVDSGFHIIGG
>Mature_257_residues
TKLLEGKNIVVMGVANERSIAWGITKSLHNAGANLIFTNRQERSYQKLVKLLDKHEIQAKLIVSCDVADDESIQQAFNEI
GEKVGVIHGVVHSVAFANRDELQGEYANTSRDGFLLAQEISAYSLVAVTKAAKELMTEGGGIVTQTYLGAERVIPGYNVM
GVAKASLEASVRYLAEDMGKYGIRVNAVSAGPIRTLSAKGVSNFNEKANVIVEKAPLRRNVDQDEVGDATLFLVSEMARG
ITGEVLHVDSGFHIIGG

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=24.8062015503876, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=45.8498023715415, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=28.7937743190661, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=29.5719844357977, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=268, Percent_Identity=29.4776119402985, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27817; Mature: 27686

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLLEGKNIVVMGVANERSIAWGITKSLHNAGANLIFTNRQERSYQKLVKLLDKHEIQA
CCCEECCCCEEEEEECCCCCEEHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCEE
KLIVSCDVADDESIQQAFNEIGEKVGVIHGVVHSVAFANRDELQGEYANTSRDGFLLAQE
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEHH
ISAYSLVAVTKAAKELMTEGGGIVTQTYLGAERVIPGYNVMGVAKASLEASVRYLAEDMG
HCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHH
KYGIRVNAVSAGPIRTLSAKGVSNFNEKANVIVEKAPLRRNVDQDEVGDATLFLVSEMAR
CCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHC
GITGEVLHVDSGFHIIGG
CCCCEEEEECCCEEEECC
>Mature Secondary Structure 
TKLLEGKNIVVMGVANERSIAWGITKSLHNAGANLIFTNRQERSYQKLVKLLDKHEIQA
CCEECCCCEEEEEECCCCCEEHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCEE
KLIVSCDVADDESIQQAFNEIGEKVGVIHGVVHSVAFANRDELQGEYANTSRDGFLLAQE
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEHH
ISAYSLVAVTKAAKELMTEGGGIVTQTYLGAERVIPGYNVMGVAKASLEASVRYLAEDMG
HCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHH
KYGIRVNAVSAGPIRTLSAKGVSNFNEKANVIVEKAPLRRNVDQDEVGDATLFLVSEMAR
CCCEEEEEECCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHC
GITGEVLHVDSGFHIIGG
CCCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]