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The map label for this gene is rpoA

Identifier: 23097600

GI number: 23097600

Start: 154474

End: 155418

Strand: Direct

Name: rpoA

Synonym: OB0145

Alternate gene names: 23097600

Gene position: 154474-155418 (Clockwise)

Preceding gene: 23097599

Following gene: 23097601

Centisome position: 4.25

GC content: 38.52

Gene sequence:

>945_bases
ATGATCGAAATCGAAAAGCCAAAGATTGAAACGGTTGAAATTAGCGAGAATGCTACATTTGGAAAATTCGTGGTTGAACC
GCTTGAACGTGGATATGGTGCCACACTTGGAAACTCCTTGCGTCGTATCTTATTATCCTCACTGCCAGGTGCTGCTGTAA
CCACAGTACAAATTGATGGAGCTTTACATGAGTTTTCAACAGTTGATGGTGTTGTAGAAGATGTTACAACTATTATCCTC
AACCTTAAGAAACTAGCTCTTAAAATTTATTCTGAAGAACCAAAAACATTAGAAATTGATGTTCAAGGTGAAGGTGCTGT
AACTGCTGCAGATTTAACGTATGACAGTGATGTGGAAGTAATGAACCCGGATTTGCATATTGCAACGCTTAATAGCAAAG
GCAATCTTCATATGAAGCTAATTGCAGAGCGAGGCCGTGGTTATCGTCCAGCAGAAGAGAATAAAAATGATGATCAACCA
ATCGGTGTCATTCCGATCGACTCCATCTTCACACCTGTTTCGCGCGTGACTTATCAAGTGGAAAACACTCGTGTAGGTCA
AGTGGCAAACTATGATAAATTAACAATGGATGTATCTACGGATGGAAGCATTCGTCCTGAAGAAGCAGTTTCTTTAGGTG
CTAAAATCATCACAGAACATCTTAATATTTTTGTAGGTTTAACAGATGAAGCACAAAATGCCGAAATCATGGTGGAAAAA
GAAGAGGATCAAAAAGAGAAGGTTATGGAAATGACCATCGAAGAACTTGATCTATCTGTTAGATCTTACAACTGCTTGAA
ACGTGCTGGAATTAACACGGTTCAAGAGCTTGCGAATAAATCAGAAGAAGATATGATGAAAGTTCGTAATTTAGGACGTA
AATCTTTAGAAGAAGTAAAAGTAAAATTAGAAGACCTAGGATTAGGTCTACGAGATGATGACTGA

Upstream 100 bases:

>100_bases
AAATACAGGTAGCGCAGAAACGCCAACTGCCTTATTTGAGGAATTTCGGTTAGACCATATGTCTAGCCGGGGTTTCGACG
TTTTAAAGGAGGGTTATGGA

Downstream 100 bases:

>100_bases
TCGAACCAGTCACCTTTAGAGTTTCGAAAAAGGGAGGGATAGTCCGTGGCTAGAAAGTTAGGACGTAAAACAGACAGTCG
TATGGCTCTTCTTCGCAACC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIEIEKPKIETVEISENATFGKFVVEPLERGYGATLGNSLRRILLSSLPGAAVTTVQIDGALHEFSTVDGVVEDVTTIIL
NLKKLALKIYSEEPKTLEIDVQGEGAVTAADLTYDSDVEVMNPDLHIATLNSKGNLHMKLIAERGRGYRPAEENKNDDQP
IGVIPIDSIFTPVSRVTYQVENTRVGQVANYDKLTMDVSTDGSIRPEEAVSLGAKIITEHLNIFVGLTDEAQNAEIMVEK
EEDQKEKVMEMTIEELDLSVRSYNCLKRAGINTVQELANKSEEDMMKVRNLGRKSLEEVKVKLEDLGLGLRDDD

Sequences:

>Translated_314_residues
MIEIEKPKIETVEISENATFGKFVVEPLERGYGATLGNSLRRILLSSLPGAAVTTVQIDGALHEFSTVDGVVEDVTTIIL
NLKKLALKIYSEEPKTLEIDVQGEGAVTAADLTYDSDVEVMNPDLHIATLNSKGNLHMKLIAERGRGYRPAEENKNDDQP
IGVIPIDSIFTPVSRVTYQVENTRVGQVANYDKLTMDVSTDGSIRPEEAVSLGAKIITEHLNIFVGLTDEAQNAEIMVEK
EEDQKEKVMEMTIEELDLSVRSYNCLKRAGINTVQELANKSEEDMMKVRNLGRKSLEEVKVKLEDLGLGLRDDD
>Mature_314_residues
MIEIEKPKIETVEISENATFGKFVVEPLERGYGATLGNSLRRILLSSLPGAAVTTVQIDGALHEFSTVDGVVEDVTTIIL
NLKKLALKIYSEEPKTLEIDVQGEGAVTAADLTYDSDVEVMNPDLHIATLNSKGNLHMKLIAERGRGYRPAEENKNDDQP
IGVIPIDSIFTPVSRVTYQVENTRVGQVANYDKLTMDVSTDGSIRPEEAVSLGAKIITEHLNIFVGLTDEAQNAEIMVEK
EEDQKEKVMEMTIEELDLSVRSYNCLKRAGINTVQELANKSEEDMMKVRNLGRKSLEEVKVKLEDLGLGLRDDD

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=312, Percent_Identity=42.9487179487179, Blast_Score=249, Evalue=1e-67,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_OCEIH (Q8ETV9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691066.1
- ProteinModelPortal:   Q8ETV9
- GeneID:   1015241
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0145
- NMPDR:   fig|221109.1.peg.146
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   OIHE221109:OB0145-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34747; Mature: 34747

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIEKPKIETVEISENATFGKFVVEPLERGYGATLGNSLRRILLSSLPGAAVTTVQIDG
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
ALHEFSTVDGVVEDVTTIILNLKKLALKIYSEEPKTLEIDVQGEGAVTAADLTYDSDVEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCEE
MNPDLHIATLNSKGNLHMKLIAERGRGYRPAEENKNDDQPIGVIPIDSIFTPVSRVTYQV
ECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHEEEEE
ENTRVGQVANYDKLTMDVSTDGSIRPEEAVSLGAKIITEHLNIFVGLTDEAQNAEIMVEK
CCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEC
EEDQKEKVMEMTIEELDLSVRSYNCLKRAGINTVQELANKSEEDMMKVRNLGRKSLEEVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
VKLEDLGLGLRDDD
HHHHHCCCCCCCCC
>Mature Secondary Structure
MIEIEKPKIETVEISENATFGKFVVEPLERGYGATLGNSLRRILLSSLPGAAVTTVQIDG
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
ALHEFSTVDGVVEDVTTIILNLKKLALKIYSEEPKTLEIDVQGEGAVTAADLTYDSDVEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCEE
MNPDLHIATLNSKGNLHMKLIAERGRGYRPAEENKNDDQPIGVIPIDSIFTPVSRVTYQV
ECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHHEEEEE
ENTRVGQVANYDKLTMDVSTDGSIRPEEAVSLGAKIITEHLNIFVGLTDEAQNAEIMVEK
CCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEC
EEDQKEKVMEMTIEELDLSVRSYNCLKRAGINTVQELANKSEEDMMKVRNLGRKSLEEVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
VKLEDLGLGLRDDD
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376