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The map label for this gene is radA [H]

Identifier: 23097549

GI number: 23097549

Start: 114315

End: 115688

Strand: Direct

Name: radA [H]

Synonym: OB0094

Alternate gene names: 23097549

Gene position: 114315-115688 (Clockwise)

Preceding gene: 23097548

Following gene: 23097550

Centisome position: 3.15

GC content: 39.45

Gene sequence:

>1374_bases
TTGGCAAAAAGAAAAACAAAATTTGTATGTCAGGAATGTGGGTATGAATCAGCTAAATGGATGGGGAAATGTCCGGCTTG
TCATCAATGGAATACCATGGTGGAGGAAATAGATCCAGCCTCAGTAAAAGGTCGGACAGGCTATGCTACTAGTACGACAT
CTGCAGGTAAGCCTGAAAAAATCACAGCCATTGAATCCAAAAAAGAACCTAGAATCACTACAAAAATGAGAGAGTTCAAC
AGAGTTCTGGGAGGCGGCATTGTACTAGGCTCACTGGTTTTAATTGGAGGAGATCCGGGTATTGGTAAATCGACACTTTT
ACTCCAAATATCCGCTCAGTTAGCAGAAAAAGATATGTCAGTACTTTATGTTTCTGGAGAGGAATCTACTCGCCAAACAA
AATTACGAGCAGATCGACTCGATATTACAACAGATCAGTTATATGTTTTATCAGAAACAAATTTATATGATATTAGTAAA
CAGGTGGAAGAAGTAAAACCTTCCTTTGTGGTTATCGATTCGATACAAACCATATTTAAAGAAGAGGTAACGAGCGCTCC
AGGAAGTGTTTCTCAAGTACGTGAATGTACAATGGAGCTTATGAAAATAGCAAAATCAAATGGGATACCTATTTTTATTG
TTGGACATGTAACGAAAGAAGGTTCCATAGCTGGTCCTCGGTTATTAGAGCATATGGTAGATGCTGTTCTGTATTTTGAA
GGCGAGCGACATCACACATATCGTATATTACGAGGAGTAAAGAACCGTTTTGGTAGTACGCATGAAATGGGAATATTTGA
GATGAAAGAAGAAGGATTACGAGAAGTTATGAATCCGTCTGAGATATTCCTAGAAGAGCGGTCAGAAGGGGCAGCAGGAT
CAACAGTTGTGGCATCGATGGAAGGAACAAGAACGGTATTGGTAGAAATACAAGCATTAATTTCTCCTACTAGTTTCGGA
AATCCACGTCGTACCGCAACAGGTATAGACCACAATCGTGTACCACTATTAATGGCTGTTTTAGAAAAGCGTGTGGGCTT
GATGCTTCAAAATCAAGACGCATATATAAAAGTTGCTGGCGGGGTAAAGTTAGATGAGCCAGCTATTGACCTTGCATTAG
CTGTAAGTATCGCATCTAGCTTTCGAGATCAACCAACAAATCCGAATGATATTTTTGTAGGTGAAGTTGGTTTAACTGGT
GAAATTAGAAGGGTTTCTCGAATTGAACAGCGTGTACAAGAAGCTGCAAAATTAGGGTTTAAACGCGTAATATGTCCAAA
GAACAATTTGGATGGCTGGAATTATCCTAGTGGAATTGAAGTCATCGGTGTATCAAGTGTACAAGAGGCTTTAGATATTG
GATTACGTCATTAA

Upstream 100 bases:

>100_bases
GGTTGTGGCATAAATATGGAAGATAAATTTGTAAATTGATAGCTTTCTGTAACCTACTACATAAAAAAATAGGCTACAAT
AAAGAGAAGGGGTAATAAAT

Downstream 100 bases:

>100_bases
TACTACTTTTTATTCTATGAAAAAACCTTATGGGATACTTTTCCAAATAATAGGCAATACTGGAGTTAGGAGGTGAGCGA
TGGTGCTTAAAAAGATAGTT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAKRKTKFVCQECGYESAKWMGKCPACHQWNTMVEEIDPASVKGRTGYATSTTSAGKPEKITAIESKKEPRITTKMREFN
RVLGGGIVLGSLVLIGGDPGIGKSTLLLQISAQLAEKDMSVLYVSGEESTRQTKLRADRLDITTDQLYVLSETNLYDISK
QVEEVKPSFVVIDSIQTIFKEEVTSAPGSVSQVRECTMELMKIAKSNGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFE
GERHHTYRILRGVKNRFGSTHEMGIFEMKEEGLREVMNPSEIFLEERSEGAAGSTVVASMEGTRTVLVEIQALISPTSFG
NPRRTATGIDHNRVPLLMAVLEKRVGLMLQNQDAYIKVAGGVKLDEPAIDLALAVSIASSFRDQPTNPNDIFVGEVGLTG
EIRRVSRIEQRVQEAAKLGFKRVICPKNNLDGWNYPSGIEVIGVSSVQEALDIGLRH

Sequences:

>Translated_457_residues
MAKRKTKFVCQECGYESAKWMGKCPACHQWNTMVEEIDPASVKGRTGYATSTTSAGKPEKITAIESKKEPRITTKMREFN
RVLGGGIVLGSLVLIGGDPGIGKSTLLLQISAQLAEKDMSVLYVSGEESTRQTKLRADRLDITTDQLYVLSETNLYDISK
QVEEVKPSFVVIDSIQTIFKEEVTSAPGSVSQVRECTMELMKIAKSNGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFE
GERHHTYRILRGVKNRFGSTHEMGIFEMKEEGLREVMNPSEIFLEERSEGAAGSTVVASMEGTRTVLVEIQALISPTSFG
NPRRTATGIDHNRVPLLMAVLEKRVGLMLQNQDAYIKVAGGVKLDEPAIDLALAVSIASSFRDQPTNPNDIFVGEVGLTG
EIRRVSRIEQRVQEAAKLGFKRVICPKNNLDGWNYPSGIEVIGVSSVQEALDIGLRH
>Mature_456_residues
AKRKTKFVCQECGYESAKWMGKCPACHQWNTMVEEIDPASVKGRTGYATSTTSAGKPEKITAIESKKEPRITTKMREFNR
VLGGGIVLGSLVLIGGDPGIGKSTLLLQISAQLAEKDMSVLYVSGEESTRQTKLRADRLDITTDQLYVLSETNLYDISKQ
VEEVKPSFVVIDSIQTIFKEEVTSAPGSVSQVRECTMELMKIAKSNGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFEG
ERHHTYRILRGVKNRFGSTHEMGIFEMKEEGLREVMNPSEIFLEERSEGAAGSTVVASMEGTRTVLVEIQALISPTSFGN
PRRTATGIDHNRVPLLMAVLEKRVGLMLQNQDAYIKVAGGVKLDEPAIDLALAVSIASSFRDQPTNPNDIFVGEVGLTGE
IRRVSRIEQRVQEAAKLGFKRVICPKNNLDGWNYPSGIEVIGVSSVQEALDIGLRH

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=459, Percent_Identity=47.276688453159, Blast_Score=423, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50242; Mature: 50111

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRKTKFVCQECGYESAKWMGKCPACHQWNTMVEEIDPASVKGRTGYATSTTSAGKPEK
CCCCHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
ITAIESKKEPRITTKMREFNRVLGGGIVLGSLVLIGGDPGIGKSTLLLQISAQLAEKDMS
EEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEEEEEEHHHHCCCCE
VLYVSGEESTRQTKLRADRLDITTDQLYVLSETNLYDISKQVEEVKPSFVVIDSIQTIFK
EEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHH
EEVTSAPGSVSQVRECTMELMKIAKSNGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFE
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
GERHHTYRILRGVKNRFGSTHEMGIFEMKEEGLREVMNPSEIFLEERSEGAAGSTVVASM
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEC
EGTRTVLVEIQALISPTSFGNPRRTATGIDHNRVPLLMAVLEKRVGLMLQNQDAYIKVAG
CCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECCCCEEEEEC
GVKLDEPAIDLALAVSIASSFRDQPTNPNDIFVGEVGLTGEIRRVSRIEQRVQEAAKLGF
CCEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
KRVICPKNNLDGWNYPSGIEVIGVSSVQEALDIGLRH
EEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKRKTKFVCQECGYESAKWMGKCPACHQWNTMVEEIDPASVKGRTGYATSTTSAGKPEK
CCCHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
ITAIESKKEPRITTKMREFNRVLGGGIVLGSLVLIGGDPGIGKSTLLLQISAQLAEKDMS
EEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCEEEEEEEHHHHCCCCE
VLYVSGEESTRQTKLRADRLDITTDQLYVLSETNLYDISKQVEEVKPSFVVIDSIQTIFK
EEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHH
EEVTSAPGSVSQVRECTMELMKIAKSNGIPIFIVGHVTKEGSIAGPRLLEHMVDAVLYFE
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
GERHHTYRILRGVKNRFGSTHEMGIFEMKEEGLREVMNPSEIFLEERSEGAAGSTVVASM
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEC
EGTRTVLVEIQALISPTSFGNPRRTATGIDHNRVPLLMAVLEKRVGLMLQNQDAYIKVAG
CCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEECCCCEEEEEC
GVKLDEPAIDLALAVSIASSFRDQPTNPNDIFVGEVGLTGEIRRVSRIEQRVQEAAKLGF
CCEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
KRVICPKNNLDGWNYPSGIEVIGVSSVQEALDIGLRH
EEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]