Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 23097542

GI number: 23097542

Start: 88663

End: 89175

Strand: Direct

Name: folK [H]

Synonym: OB0087

Alternate gene names: 23097542

Gene position: 88663-89175 (Clockwise)

Preceding gene: 23097541

Following gene: 28563899

Centisome position: 2.44

GC content: 36.84

Gene sequence:

>513_bases
ATGAATGAAGCTTATGTAGCCTTAGGCACAAATATTGAACCGCGAGAAAACTTTATTAATCAAGCACTGCAATTTTTGGA
TGACCACCCAAACATAACCATTAAGGAAAAGTCTTCTATATATGAAACGGCGCCTGTAGGTTACACAGAACAATCCAACT
TTTTAAATATGGTAGTGCAAGTAATGACGAGTTTATCCGCAAATGAACTATTAGATGCATGTCAATGGATTGAACAGGAG
TTAGGAAGAGTAAGAACGATTCGATTTGGTCCTCGTACAATCGACCTTGACATTTTGACGTATAATCAAGAAAATAGTAC
AGTAGAAAGATTGATGTTGCCGCATCCTAGAATGCATGAACGCGCATTTGTTTTGATACCACTTCAAGAAATCGCACCAG
GCTATATAATCCCTGTGAAAAATAAAACAGTGGATTCTTATGTCAATGAACTTCCTGAGACAGAGAAAAAGGATGTAAAA
AGATGGATAAGAAGCGAATCGGAAGAAGAATAA

Upstream 100 bases:

>100_bases
CATTATTGAATGCTTGTTTAGTGAGAGTAGTAAAACCAGACCCGCCAATTCCGGGGCATTATCAATCTGTTGCTGTAGAA
ATTTATAGGGAGAGGGAAAA

Downstream 100 bases:

>100_bases
AAGCTTTTCGTAAATTAAAAGGCTACACACAAATACAATTTGCTCAAGAGATGAAGCTATCCACTTCAAAACTAGGAAAT
ATTGAAAGAGGTACAAAGCA

Product: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridin e pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MNEAYVALGTNIEPRENFINQALQFLDDHPNITIKEKSSIYETAPVGYTEQSNFLNMVVQVMTSLSANELLDACQWIEQE
LGRVRTIRFGPRTIDLDILTYNQENSTVERLMLPHPRMHERAFVLIPLQEIAPGYIIPVKNKTVDSYVNELPETEKKDVK
RWIRSESEEE

Sequences:

>Translated_170_residues
MNEAYVALGTNIEPRENFINQALQFLDDHPNITIKEKSSIYETAPVGYTEQSNFLNMVVQVMTSLSANELLDACQWIEQE
LGRVRTIRFGPRTIDLDILTYNQENSTVERLMLPHPRMHERAFVLIPLQEIAPGYIIPVKNKTVDSYVNELPETEKKDVK
RWIRSESEEE
>Mature_170_residues
MNEAYVALGTNIEPRENFINQALQFLDDHPNITIKEKSSIYETAPVGYTEQSNFLNMVVQVMTSLSANELLDACQWIEQE
LGRVRTIRFGPRTIDLDILTYNQENSTVERLMLPHPRMHERAFVLIPLQEIAPGYIIPVKNKTVDSYVNELPETEKKDVK
RWIRSESEEE

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=44.6043165467626, Blast_Score=111, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324073, Length=168, Percent_Identity=38.0952380952381, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 19710; Mature: 19710

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAYVALGTNIEPRENFINQALQFLDDHPNITIKEKSSIYETAPVGYTEQSNFLNMVVQ
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
VMTSLSANELLDACQWIEQELGRVRTIRFGPRTIDLDILTYNQENSTVERLMLPHPRMHE
HHHCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEEECCCCHHHHHHHCCCCCCCC
RAFVLIPLQEIAPGYIIPVKNKTVDSYVNELPETEKKDVKRWIRSESEEE
CEEEEEEHHHHCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNEAYVALGTNIEPRENFINQALQFLDDHPNITIKEKSSIYETAPVGYTEQSNFLNMVVQ
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHH
VMTSLSANELLDACQWIEQELGRVRTIRFGPRTIDLDILTYNQENSTVERLMLPHPRMHE
HHHCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEEEEECCCCHHHHHHHCCCCCCCC
RAFVLIPLQEIAPGYIIPVKNKTVDSYVNELPETEKKDVKRWIRSESEEE
CEEEEEEHHHHCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]