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The map label for this gene is pth

Identifier: 23097516

GI number: 23097516

Start: 59594

End: 60157

Strand: Direct

Name: pth

Synonym: OB0061

Alternate gene names: 23097516

Gene position: 59594-60157 (Clockwise)

Preceding gene: 23097515

Following gene: 23097517

Centisome position: 1.64

GC content: 35.82

Gene sequence:

>564_bases
ATGAAACTAATTGTTGGTCTGGGGAACCCAGGAAGAAAGTTTAAAAAAACAAGACATAATATTGGTTTTTTTGTCATTGA
TGAATTATTACAACGTCATAAGTGGAAGCTAAATAATTCGAAGTTTGAAGGGGATTATTCCATAGAACATTTCGATGGTG
AGAAAGTCATCCTATTACAACCTCAAACATATATGAATCTCTCTGGTAAATCGATCAGTCCGTTAATGGACTATTTTGAT
ATAGATGTCGATGATGTGATAGTTGTTTATGATGATCTGGATTTACCAACGGGAAAAATTAGACTTCGCCAAAAAGGTGG
TCATGGTGGACACAATGGTGTACGATCTACGATTGATCATTTAGGTACAAAGGATTTCAAAAGAGTTCGTTTAGGAATAG
GAAGACCGACGAACGCAACTCCAGTAATTGATTATGTATTAGGTAAATTTCCAAAGCAAGAGACAACTGCAGTAACAGAT
AGTGTGGAAAAGGCGGCTGATGCTATCGAAGCCTGGATAAAAGGTAAACCATTCTTAGAAGTTATGAATGATTTTAACCA
GTAA

Upstream 100 bases:

>100_bases
AAAAGTACGGGTTTAAAAAGAAGAAAATGATTATGTTTGGAAAAATTAACAAATTATTTTTTAGATTTTTTTGAACATCG
TCTGTAAAGGAAGAATAAAA

Downstream 100 bases:

>100_bases
AGCATAGCTTATCATATATTAAGTATATTCTAATACAACCCGGTTAAACTGTTAATAACAGATCGTTTAACTGGGGTGGT
AAAATGGCAATTTATTATGA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKLIVGLGNPGRKFKKTRHNIGFFVIDELLQRHKWKLNNSKFEGDYSIEHFDGEKVILLQPQTYMNLSGKSISPLMDYFD
IDVDDVIVVYDDLDLPTGKIRLRQKGGHGGHNGVRSTIDHLGTKDFKRVRLGIGRPTNATPVIDYVLGKFPKQETTAVTD
SVEKAADAIEAWIKGKPFLEVMNDFNQ

Sequences:

>Translated_187_residues
MKLIVGLGNPGRKFKKTRHNIGFFVIDELLQRHKWKLNNSKFEGDYSIEHFDGEKVILLQPQTYMNLSGKSISPLMDYFD
IDVDDVIVVYDDLDLPTGKIRLRQKGGHGGHNGVRSTIDHLGTKDFKRVRLGIGRPTNATPVIDYVLGKFPKQETTAVTD
SVEKAADAIEAWIKGKPFLEVMNDFNQ
>Mature_187_residues
MKLIVGLGNPGRKFKKTRHNIGFFVIDELLQRHKWKLNNSKFEGDYSIEHFDGEKVILLQPQTYMNLSGKSISPLMDYFD
IDVDDVIVVYDDLDLPTGKIRLRQKGGHGGHNGVRSTIDHLGTKDFKRVRLGIGRPTNATPVIDYVLGKFPKQETTAVTD
SVEKAADAIEAWIKGKPFLEVMNDFNQ

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=171, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=3e-25,
Organism=Escherichia coli, GI1787455, Length=174, Percent_Identity=42.5287356321839, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321983, Length=184, Percent_Identity=27.7173913043478, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_OCEIH (Q8CXP8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690982.1
- ProteinModelPortal:   Q8CXP8
- SMR:   Q8CXP8
- GeneID:   1015122
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0061
- NMPDR:   fig|221109.1.peg.61
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   OIHE221109:OB0061-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21158; Mature: 21158

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPGRKFKKTRHNIGFFVIDELLQRHKWKLNNSKFEGDYSIEHFDGEKVILLQ
CEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEEEC
PQTYMNLSGKSISPLMDYFDIDVDDVIVVYDDLDLPTGKIRLRQKGGHGGHNGVRSTIDH
CCHHCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHH
LGTKDFKRVRLGIGRPTNATPVIDYVLGKFPKQETTAVTDSVEKAADAIEAWIKGKPFLE
CCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VMNDFNQ
HHHHCCC
>Mature Secondary Structure
MKLIVGLGNPGRKFKKTRHNIGFFVIDELLQRHKWKLNNSKFEGDYSIEHFDGEKVILLQ
CEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEEEC
PQTYMNLSGKSISPLMDYFDIDVDDVIVVYDDLDLPTGKIRLRQKGGHGGHNGVRSTIDH
CCHHCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHH
LGTKDFKRVRLGIGRPTNATPVIDYVLGKFPKQETTAVTDSVEKAADAIEAWIKGKPFLE
CCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VMNDFNQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376