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The map label for this gene is ksgA

Identifier: 23097505

GI number: 23097505

Start: 50456

End: 51340

Strand: Direct

Name: ksgA

Synonym: OB0050

Alternate gene names: 23097505

Gene position: 50456-51340 (Clockwise)

Preceding gene: 23097504

Following gene: 23097506

Centisome position: 1.39

GC content: 36.38

Gene sequence:

>885_bases
ATGACGACGAACAAATACATCGCAACTCCTTCTAGAACGAAGGATATTTTAGGTAAATATCATTTCACATTTAAAAAAAG
TTTAGGACAAAACTTTTTAGTGGATGTTTCGGTATTACAAAATATCATTCGCCATGCAGGAATTACAAAAGATACTGCTG
CAATTGAAATTGGTCCGGGTATTGGAGCACTGACAGAGCAATTAGCAATACATGCGGATCAAGTGGTTGCATTTGAAATT
GATCAACGATTATTACCAATTTTACAAGACACATTGGGTGAATATTCGAATGTATCTGTGATTCATCAAGATATTTTAAA
GGCGGATGTGACAAAGGTTATTGACGAACATTTTAAGGAAGGTCAAGAAGTTCATGTTGTTGCAAATTTACCATATTACA
TTACGACCCCTATTTTAATGAAATTGATTCGTGATCGGTTACCAGTAACAAGTTTAACGGTTATGATTCAAAAAGAAGTA
GCAGATCGTATGTCTGGCGAACCGAATTCAAAAAGCTATGGTTCCTTAAGTCTTGCGGTCCAATATTACAGTGAAGCAAA
AGTAGTAATGAACGTACCGAAGCAAGTGTTTATGCCGCAACCGAATGTTGATTCAAGTATATTGCAACTAACAATGCGAA
AACAGCCTCCTGTGGAAGTTACGGATGAAGATTTCTTCTTTGAAATTATTCAAGCAAGCTTTGCGCAACGCCGTAAAACA
CTAAAGAATAATCTCACTCGCTTTTTTAAAGGGGTTCATGACAAGGAAAAGATTGACCATATATTACAAGAAGCAGGTGT
GGAAGGGATTCGAAGAGGAGAGTCTCTAACTATGGAGGAATTCGCGCAAGTAGCAAATACTTTTTACCAATACCAATCTA
AATAA

Upstream 100 bases:

>100_bases
CATACAAATGGCAAGCAATTATTAAAACGACTTACACAGTTCCAAATTAGCAAAAAACAGTTAGATGAAGCTATGGAATT
AATGGATCAGGAGGTTCAAC

Downstream 100 bases:

>100_bases
TACTACAATGACTCGAGTGTTAAGGCACAATAGCCTGCTTTTTCTCATATGGTATAGGAGACATATCGTGTGAGGTGTAC
AGATGGTTATTGCAAACGGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTTNKYIATPSRTKDILGKYHFTFKKSLGQNFLVDVSVLQNIIRHAGITKDTAAIEIGPGIGALTEQLAIHADQVVAFEI
DQRLLPILQDTLGEYSNVSVIHQDILKADVTKVIDEHFKEGQEVHVVANLPYYITTPILMKLIRDRLPVTSLTVMIQKEV
ADRMSGEPNSKSYGSLSLAVQYYSEAKVVMNVPKQVFMPQPNVDSSILQLTMRKQPPVEVTDEDFFFEIIQASFAQRRKT
LKNNLTRFFKGVHDKEKIDHILQEAGVEGIRRGESLTMEEFAQVANTFYQYQSK

Sequences:

>Translated_294_residues
MTTNKYIATPSRTKDILGKYHFTFKKSLGQNFLVDVSVLQNIIRHAGITKDTAAIEIGPGIGALTEQLAIHADQVVAFEI
DQRLLPILQDTLGEYSNVSVIHQDILKADVTKVIDEHFKEGQEVHVVANLPYYITTPILMKLIRDRLPVTSLTVMIQKEV
ADRMSGEPNSKSYGSLSLAVQYYSEAKVVMNVPKQVFMPQPNVDSSILQLTMRKQPPVEVTDEDFFFEIIQASFAQRRKT
LKNNLTRFFKGVHDKEKIDHILQEAGVEGIRRGESLTMEEFAQVANTFYQYQSK
>Mature_293_residues
TTNKYIATPSRTKDILGKYHFTFKKSLGQNFLVDVSVLQNIIRHAGITKDTAAIEIGPGIGALTEQLAIHADQVVAFEID
QRLLPILQDTLGEYSNVSVIHQDILKADVTKVIDEHFKEGQEVHVVANLPYYITTPILMKLIRDRLPVTSLTVMIQKEVA
DRMSGEPNSKSYGSLSLAVQYYSEAKVVMNVPKQVFMPQPNVDSSILQLTMRKQPPVEVTDEDFFFEIIQASFAQRRKTL
KNNLTRFFKGVHDKEKIDHILQEAGVEGIRRGESLTMEEFAQVANTFYQYQSK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=305, Percent_Identity=28.1967213114754, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI7657198, Length=290, Percent_Identity=27.5862068965517, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=33.587786259542, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=299, Percent_Identity=28.4280936454849, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25146882, Length=222, Percent_Identity=29.7297297297297, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=309, Percent_Identity=27.831715210356, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21358017, Length=235, Percent_Identity=31.063829787234, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357273, Length=290, Percent_Identity=25.8620689655172, Blast_Score=104, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_OCEIH (P59155)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690971.1
- ProteinModelPortal:   P59155
- SMR:   P59155
- GeneID:   1015111
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0050
- NMPDR:   fig|221109.1.peg.50
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   OIHE221109:OB0050-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33291; Mature: 33160

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 31-31 BINDING 33-33 BINDING 58-58 BINDING 79-79 BINDING 104-104 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNKYIATPSRTKDILGKYHFTFKKSLGQNFLVDVSVLQNIIRHAGITKDTAAIEIGPG
CCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCEEEECCC
IGALTEQLAIHADQVVAFEIDQRLLPILQDTLGEYSNVSVIHQDILKADVTKVIDEHFKE
HHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GQEVHVVANLPYYITTPILMKLIRDRLPVTSLTVMIQKEVADRMSGEPNSKSYGSLSLAV
CCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEE
QYYSEAKVVMNVPKQVFMPQPNVDSSILQLTMRKQPPVEVTDEDFFFEIIQASFAQRRKT
HHHHCCHHHHHCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LKNNLTRFFKGVHDKEKIDHILQEAGVEGIRRGESLTMEEFAQVANTFYQYQSK
HHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTNKYIATPSRTKDILGKYHFTFKKSLGQNFLVDVSVLQNIIRHAGITKDTAAIEIGPG
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCEEEECCC
IGALTEQLAIHADQVVAFEIDQRLLPILQDTLGEYSNVSVIHQDILKADVTKVIDEHFKE
HHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GQEVHVVANLPYYITTPILMKLIRDRLPVTSLTVMIQKEVADRMSGEPNSKSYGSLSLAV
CCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEE
QYYSEAKVVMNVPKQVFMPQPNVDSSILQLTMRKQPPVEVTDEDFFFEIIQASFAQRRKT
HHHHCCHHHHHCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LKNNLTRFFKGVHDKEKIDHILQEAGVEGIRRGESLTMEEFAQVANTFYQYQSK
HHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376