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The map label for this gene is recF

Identifier: 23097459

GI number: 23097459

Start: 3443

End: 4552

Strand: Direct

Name: recF

Synonym: OB0004

Alternate gene names: 23097459

Gene position: 3443-4552 (Clockwise)

Preceding gene: 23097458

Following gene: 23097460

Centisome position: 0.09

GC content: 34.95

Gene sequence:

>1110_bases
ATGCATATTGAAAAATTAGAACTAACGAATTATCGAAATTATGACCAATTGGAGATAGCATTTGATGATCAAATTAATGT
GATTATCGGAGAAAATGCTCAAGGTAAAACAAATCTAATGGAAGCTATCTACGTTCTATCATTTGCTCGGAGTCACCGAA
CTCCTAGAGAAAAAGAATTAATTCAATGGGACAAAGATTATGCTAAAATAGAAGGTAGAATTACAAAAAGAAATCAGTCT
ATTCCCTTGCAAATCTCCATAACTTCCAAAGGAAAAAAAGCAAAGGTCAATCACCTTGAGCAACATCGTTTAAGCGACTA
TATTGGGTCGGTGAATGTCGTGATGTTTGCTCCAGAAGATCTGACAATTGTCAAAGGTGCTCCCCAAATTCGACGACGAT
TTATGGACATGGAACTTGGTCAAATACAACCAACCTATATTTATCATTTAGCGCAGTATCAAAAAGTATTAAAGCAACGC
AACCATTTATTAAAACAATTACAACGAAAACCTAATTCCGATACGACCATGTTAGAAGTATTAACCGATCAATTGATTGA
GCATGCTTCTATTTTATTGGAACGACGTTTTATTTACTTAGAATTGTTAAGAAAATGGGCTCAACCGATTCATAGAGGAA
TAAGTCGAGAATTAGAGCAACTCGAAATTCAATATAGTCCGAGTATTGAAGTATCAGAAGACGCAAATAAGGAAAAAATA
GGTAATATATATCAAATGAAGTTCGCAGAGGTAAAGCAAAAGGAGATTGAGCGTGGAACGACTTTAGCTGGCCCGCATCG
AGATGATTTAATTTTCTTTGTAAATGGAAAAGATGTGCAAACATATGGTTCACAAGGTCAGCAGCGCACAACTGCTTTAT
CGATTAAACTAGCGGAAATTGAGCTGATTTATCAAGAGGTTGGAGAATATCCTATCCTCCTGCTAGATGATGTATTAAGT
GAATTAGATGATTATCGTCAATCTCATTTACTTAATACTATTCAAGGTAAGGTACAAACATTTGTATCAACGACAAGTGT
TGAAGGTATTCATCATGAAACATTACAACAAGCTGAGTTATTTAGAGTTACCGATGGTGTAGTGAATTAA

Upstream 100 bases:

>100_bases
ATGGTCATCTCGAGCAAAGACGTGGAAGAAAGTTATATCCAAAGGATGTCGTTGAAATCCAAGGCATTGGTTCATATATC
GTGATTAAAGAGGACTAAAC

Downstream 100 bases:

>100_bases
TTAGCAGAAAATAATTTAAAAGGAAGTGACGATTTATGTTTATCCATATTGGAAACGGAAATGTCATTCGAACAAAGGAG
ATCGTTGCCATTATTGATTG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MHIEKLELTNYRNYDQLEIAFDDQINVIIGENAQGKTNLMEAIYVLSFARSHRTPREKELIQWDKDYAKIEGRITKRNQS
IPLQISITSKGKKAKVNHLEQHRLSDYIGSVNVVMFAPEDLTIVKGAPQIRRRFMDMELGQIQPTYIYHLAQYQKVLKQR
NHLLKQLQRKPNSDTTMLEVLTDQLIEHASILLERRFIYLELLRKWAQPIHRGISRELEQLEIQYSPSIEVSEDANKEKI
GNIYQMKFAEVKQKEIERGTTLAGPHRDDLIFFVNGKDVQTYGSQGQQRTTALSIKLAEIELIYQEVGEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVSTTSVEGIHHETLQQAELFRVTDGVVN

Sequences:

>Translated_369_residues
MHIEKLELTNYRNYDQLEIAFDDQINVIIGENAQGKTNLMEAIYVLSFARSHRTPREKELIQWDKDYAKIEGRITKRNQS
IPLQISITSKGKKAKVNHLEQHRLSDYIGSVNVVMFAPEDLTIVKGAPQIRRRFMDMELGQIQPTYIYHLAQYQKVLKQR
NHLLKQLQRKPNSDTTMLEVLTDQLIEHASILLERRFIYLELLRKWAQPIHRGISRELEQLEIQYSPSIEVSEDANKEKI
GNIYQMKFAEVKQKEIERGTTLAGPHRDDLIFFVNGKDVQTYGSQGQQRTTALSIKLAEIELIYQEVGEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVSTTSVEGIHHETLQQAELFRVTDGVVN
>Mature_369_residues
MHIEKLELTNYRNYDQLEIAFDDQINVIIGENAQGKTNLMEAIYVLSFARSHRTPREKELIQWDKDYAKIEGRITKRNQS
IPLQISITSKGKKAKVNHLEQHRLSDYIGSVNVVMFAPEDLTIVKGAPQIRRRFMDMELGQIQPTYIYHLAQYQKVLKQR
NHLLKQLQRKPNSDTTMLEVLTDQLIEHASILLERRFIYLELLRKWAQPIHRGISRELEQLEIQYSPSIEVSEDANKEKI
GNIYQMKFAEVKQKEIERGTTLAGPHRDDLIFFVNGKDVQTYGSQGQQRTTALSIKLAEIELIYQEVGEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVSTTSVEGIHHETLQQAELFRVTDGVVN

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=375, Percent_Identity=24.8, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_OCEIH (Q8EU85)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_690925.1
- ProteinModelPortal:   Q8EU85
- SMR:   Q8EU85
- GeneID:   1015028
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0004
- NMPDR:   fig|221109.1.peg.4
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   OIHE221109:OB0004-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42829; Mature: 42829

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIEKLELTNYRNYDQLEIAFDDQINVIIGENAQGKTNLMEAIYVLSFARSHRTPREKEL
CCCCEEECCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
IQWDKDYAKIEGRITKRNQSIPLQISITSKGKKAKVNHLEQHRLSDYIGSVNVVMFAPED
HHHCHHHHHHCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LTIVKGAPQIRRRFMDMELGQIQPTYIYHLAQYQKVLKQRNHLLKQLQRKPNSDTTMLEV
EEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LTDQLIEHASILLERRFIYLELLRKWAQPIHRGISRELEQLEIQYSPSIEVSEDANKEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECCCCCHHHH
GNIYQMKFAEVKQKEIERGTTLAGPHRDDLIFFVNGKDVQTYGSQGQQRTTALSIKLAEI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEHHCCCCCCEEEEEEEEHHHH
ELIYQEVGEYPILLLDDVLSELDDYRQSHLLNTIQGKVQTFVSTTSVEGIHHETLQQAEL
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FRVTDGVVN
HHHHCCCCC
>Mature Secondary Structure
MHIEKLELTNYRNYDQLEIAFDDQINVIIGENAQGKTNLMEAIYVLSFARSHRTPREKEL
CCCCEEECCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
IQWDKDYAKIEGRITKRNQSIPLQISITSKGKKAKVNHLEQHRLSDYIGSVNVVMFAPED
HHHCHHHHHHCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LTIVKGAPQIRRRFMDMELGQIQPTYIYHLAQYQKVLKQRNHLLKQLQRKPNSDTTMLEV
EEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LTDQLIEHASILLERRFIYLELLRKWAQPIHRGISRELEQLEIQYSPSIEVSEDANKEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEECCCCCHHHH
GNIYQMKFAEVKQKEIERGTTLAGPHRDDLIFFVNGKDVQTYGSQGQQRTTALSIKLAEI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEHHCCCCCCEEEEEEEEHHHH
ELIYQEVGEYPILLLDDVLSELDDYRQSHLLNTIQGKVQTFVSTTSVEGIHHETLQQAEL
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FRVTDGVVN
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376