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The map label for this gene is gidB [H]

Identifier: 22128011

GI number: 22128011

Start: 4597740

End: 4598411

Strand: Reverse

Name: gidB [H]

Synonym: y4143

Alternate gene names: 22128011

Gene position: 4598411-4597740 (Counterclockwise)

Preceding gene: 161484718

Following gene: 161484719

Centisome position: 99.95

GC content: 45.68

Gene sequence:

>672_bases
TTGACCCATTTATATGCTGACTGGGCGTTTTCTGGTCAGAGGATACCCTTTGTGTTAAAAAAATTAGACTCTCTGCTTAC
CGTTGCTGGTATTACGTTACCCGATCAGCAAAAACATCAGTTGATTGGCTATGTAGAGCTGCTAGACAAGTGGAATAAAG
CTTATAACCTGACATCTGTTCGTGATCCACAGCAAATGTTAGTGCGTCATATCCTTGATAGCATTGTGGTTAATCCCCAT
CTGCAAGGCAGCCGATTTATTGATGTCGGAACCGGGCCAGGTCTTCCTGGTATCCCTTTGGCTATTGTGCGCCCTGATGC
ACATTTTACTTTGTTGGATAGCCTGGGTAAGCGGGTTCGCTTTCTACGTCAGGTGCAACACGAACTGGGGTTAAATAACA
TTGAGCCGGTTCAGAGCAGAGTTGAGGCATTTACCTCAGAACCGCCATTTGATGGTGTGATAAGCCGTGCATTTGCATCC
TTACAAGATATGCTCTCGTGGTGTCACCACTTGCCAGCTAAGCCTGAAGGGCGTTTTTATGCGCTTAAAGGGGTTCGGCC
TGATGATGAGTTAGCGGTTCTGCCCGAAGATATTGTTCTTGAGTCGGTGATAAAACTGGATGTTCCCGAATTGGATGGAG
AGCGGCATTTGATTATTCTTAAGTCAAACTAA

Upstream 100 bases:

>100_bases
GCGTGACGATTAAACGTTTGCCTGCACCCTGTATTTTACGGTCAGGCAAATACAGTAAACATATTCAATCCATTACCATG
CTGGATTATCATTTTGCCTA

Downstream 100 bases:

>100_bases
CTTTGCTTTTTATCAAAAAAAGGCAACAAATTATTAGTAGTCGTTAGTAAACTTATTGAGTTCATTGTTTCCAGCATGGG
GAGATCTTTATTTGCTCACT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTHLYADWAFSGQRIPFVLKKLDSLLTVAGITLPDQQKHQLIGYVELLDKWNKAYNLTSVRDPQQMLVRHILDSIVVNPH
LQGSRFIDVGTGPGLPGIPLAIVRPDAHFTLLDSLGKRVRFLRQVQHELGLNNIEPVQSRVEAFTSEPPFDGVISRAFAS
LQDMLSWCHHLPAKPEGRFYALKGVRPDDELAVLPEDIVLESVIKLDVPELDGERHLIILKSN

Sequences:

>Translated_223_residues
MTHLYADWAFSGQRIPFVLKKLDSLLTVAGITLPDQQKHQLIGYVELLDKWNKAYNLTSVRDPQQMLVRHILDSIVVNPH
LQGSRFIDVGTGPGLPGIPLAIVRPDAHFTLLDSLGKRVRFLRQVQHELGLNNIEPVQSRVEAFTSEPPFDGVISRAFAS
LQDMLSWCHHLPAKPEGRFYALKGVRPDDELAVLPEDIVLESVIKLDVPELDGERHLIILKSN
>Mature_222_residues
THLYADWAFSGQRIPFVLKKLDSLLTVAGITLPDQQKHQLIGYVELLDKWNKAYNLTSVRDPQQMLVRHILDSIVVNPHL
QGSRFIDVGTGPGLPGIPLAIVRPDAHFTLLDSLGKRVRFLRQVQHELGLNNIEPVQSRVEAFTSEPPFDGVISRAFASL
QDMLSWCHHLPAKPEGRFYALKGVRPDDELAVLPEDIVLESVIKLDVPELDGERHLIILKSN

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=206, Percent_Identity=77.1844660194175, Blast_Score=327, Evalue=3e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 25087; Mature: 24956

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLYADWAFSGQRIPFVLKKLDSLLTVAGITLPDQQKHQLIGYVELLDKWNKAYNLTSV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
RDPQQMLVRHILDSIVVNPHLQGSRFIDVGTGPGLPGIPLAIVRPDAHFTLLDSLGKRVR
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
FLRQVQHELGLNNIEPVQSRVEAFTSEPPFDGVISRAFASLQDMLSWCHHLPAKPEGRFY
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ALKGVRPDDELAVLPEDIVLESVIKLDVPELDGERHLIILKSN
EEECCCCCCCEEECCHHHHHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
THLYADWAFSGQRIPFVLKKLDSLLTVAGITLPDQQKHQLIGYVELLDKWNKAYNLTSV
CCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
RDPQQMLVRHILDSIVVNPHLQGSRFIDVGTGPGLPGIPLAIVRPDAHFTLLDSLGKRVR
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
FLRQVQHELGLNNIEPVQSRVEAFTSEPPFDGVISRAFASLQDMLSWCHHLPAKPEGRFY
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ALKGVRPDDELAVLPEDIVLESVIKLDVPELDGERHLIILKSN
EEECCCCCCCEEECCHHHHHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA