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The map label for this gene is mscL [H]

Identifier: 22127889

GI number: 22127889

Start: 4457708

End: 4458130

Strand: Reverse

Name: mscL [H]

Synonym: y4019

Alternate gene names: 22127889

Gene position: 4458130-4457708 (Counterclockwise)

Preceding gene: 22127890

Following gene: 22127881

Centisome position: 96.9

GC content: 42.32

Gene sequence:

>423_bases
GTGTTGGCTATGAGCTTTATGAAAGAATTTCGTGAATTTGCCATGCGTGGCAATGTGGTTGACTTAGCTGTTGGTGTGAT
TATTGGTGCTGCATTCGGCAGAATTGTCTCTTCGCTCGTTGCCGATATTATCATGCCACCACTGGGGTTATTGCTAGGCG
GGGTAGATTTTAAACAATTCCATTTTGTATTACGAGCCGCTGAAGGGACTATACCCGCGGTAGTAATGAATTATGGTACA
TTTATTCAAAGCATTTTTGATTTTGTTATTGTAGCCCTCGCTATATTCTCCGCTGTTAAGTTAATGAATAAGCTACGCCG
TGAAAAAGCGGAAGAAGAACCTGCTACACCGCCAGCACCAACGACTGAAGAGATACTATTAGCTGAGATTCGCGATTTAT
TAAAAGCGCAGCACACAAAATAG

Upstream 100 bases:

>100_bases
TAAAAAGCCATGCTCATGAGGAAATAATTAAAATTTCCCTATAATTTTAGTGATAATAGGCATTATTTAGCTAGAATTAA
GTCATTCATTTATTGATGGG

Downstream 100 bases:

>100_bases
TTTCAGTTTAGCTAAATTTAAGAGCTACCGGAATGAGTTGCCCTAAACAGCAACACAAAAGCGGGTATTTGGATTAAGTC
CAAGAAGATAACTTTTTATT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLAMSFMKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRAAEGTIPAVVMNYGT
FIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEILLAEIRDLLKAQHTK

Sequences:

>Translated_140_residues
MLAMSFMKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRAAEGTIPAVVMNYGT
FIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEILLAEIRDLLKAQHTK
>Mature_140_residues
MLAMSFMKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQFHFVLRAAEGTIPAVVMNYGT
FIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAPTTEEILLAEIRDLLKAQHTK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=137, Percent_Identity=75.9124087591241, Blast_Score=210, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15365; Mature: 15365

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAMSFMKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQF
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HFVLRAAEGTIPAVVMNYGTFIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTEEILLAEIRDLLKAQHTK
CHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAMSFMKEFREFAMRGNVVDLAVGVIIGAAFGRIVSSLVADIIMPPLGLLLGGVDFKQF
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HFVLRAAEGTIPAVVMNYGTFIQSIFDFVIVALAIFSAVKLMNKLRREKAEEEPATPPAP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TTEEILLAEIRDLLKAQHTK
CHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA