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The map label for this gene is bioH [H]

Identifier: 22127778

GI number: 22127778

Start: 4363541

End: 4364188

Strand: Direct

Name: bioH [H]

Synonym: y3908

Alternate gene names: 22127778

Gene position: 4363541-4364188 (Clockwise)

Preceding gene: 22127777

Following gene: 22127779

Centisome position: 94.84

GC content: 52.78

Gene sequence:

>648_bases
TTGGTGGACCTGCCGGGTTATGGGCGCAGCCAAGATTATGGTGCGATGTCACTGGCTGACATGGCCGAGAGAGTGGCACA
GCAAGCGCCGAAACAGGCATTGTGGCTTGGGTGGTCAATGGGGGGATTGGTCGCTAGCCAAATAGCCCTGAGCCAGCCAG
AGTGTGTCAGGGGGCTGATCACCGTCTCCTCTTCTCCCTGCTTTACCGCGCGTGATGAATGGCCGGGTATCAAGCCAGAA
GTGCTGGCAGGTTTTCAACATCAGCTAAGTGATGATTTTCATCGGACAGTAGAACGCTTTTTGGCCTTGCAAACCTTAGG
GACTGAAAGTTCGCGTCAGGATGCCCGTTTGCTAAAATCTGTCGTGCTACAGCATCAAATGCCCGATGTTGAGGTATTGA
CCGGGGGGTTGGCGATCTTGCGCACGGCGGATCTACGTACAGCACTGGCTGGTTTTACGCTGCCTTTTATGCGCGTTTAC
GGTCATCTGGACAGTTTGGTCCCACGTAAAGTGGCATCGCTATTAGACAGCGCCTGGCCGCAAACCCAGTCGGTGGTCAT
GCAAGGGGCTGCTCATGCCCCCTTTATTTCTCACCCCAATGATTTCGCCAAATTAATCCTGAATTTTGCTGAAGAAAACA
AAAAATAA

Upstream 100 bases:

>100_bases
GAAGGCGATTGCGATCTTGTGTTGTTGCACGGATGGGGGCTGAATAGCGGGGGTATGGCATTGCATTATTGATCGACTTG
CGCCGCATTTTCGTCTGCAT

Downstream 100 bases:

>100_bases
TGGCGTTAGCTTCTTATTTTCTCTCTCTATTTTTACTGCCCAGCCTATATTGGTTGGGTAAAATGGTTGTGTAAAAGAAA
ATTATTTAAGTTATTAAATG

Product: biotin biosynthesis protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGIKPE
VLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKSVVLQHQMPDVEVLTGGLAILRTADLRTALAGFTLPFMRVY
GHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFISHPNDFAKLILNFAEENKK

Sequences:

>Translated_215_residues
MVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGIKPE
VLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKSVVLQHQMPDVEVLTGGLAILRTADLRTALAGFTLPFMRVY
GHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFISHPNDFAKLILNFAEENKK
>Mature_215_residues
MVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLITVSSSPCFTARDEWPGIKPE
VLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKSVVLQHQMPDVEVLTGGLAILRTADLRTALAGFTLPFMRVY
GHLDSLVPRKVASLLDSAWPQTQSVVMQGAAHAPFISHPNDFAKLILNFAEENKK

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=211, Percent_Identity=65.8767772511848, Blast_Score=299, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 23571; Mature: 23571

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
TVSSSPCFTARDEWPGIKPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKS
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VVLQHQMPDVEVLTGGLAILRTADLRTALAGFTLPFMRVYGHLDSLVPRKVASLLDSAWP
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTQSVVMQGAAHAPFISHPNDFAKLILNFAEENKK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDLPGYGRSQDYGAMSLADMAERVAQQAPKQALWLGWSMGGLVASQIALSQPECVRGLI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
TVSSSPCFTARDEWPGIKPEVLAGFQHQLSDDFHRTVERFLALQTLGTESSRQDARLLKS
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VVLQHQMPDVEVLTGGLAILRTADLRTALAGFTLPFMRVYGHLDSLVPRKVASLLDSAWP
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTQSVVMQGAAHAPFISHPNDFAKLILNFAEENKK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA