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The map label for this gene is pulD [H]

Identifier: 22127080

GI number: 22127080

Start: 3523708

End: 3525630

Strand: Reverse

Name: pulD [H]

Synonym: y3204

Alternate gene names: 22127080

Gene position: 3525630-3523708 (Counterclockwise)

Preceding gene: 22127081

Following gene: 22127079

Centisome position: 76.63

GC content: 33.33

Gene sequence:

>1923_bases
ATGTATATTAGTGGCAAAGGAATTAAATCTATCCATGGGATGATTTTTTTATTCACTCTAATTATGCCATTAGACATAAT
AAGTGCTAATTTTTCAGTCTCTTTCAAAGATGTGGATATAAAAGAGTTTATTAATTCAGTAAGTAAGAATATTAATAAAA
CTATTATCATAGATCCTACGGTCCAAGGGCTTATCAGCATTCGCAGTTATGAGAATTTGGATAAAGATACGTATTATCAA
CTATTCTTGAATGTGCTTGATGTCTATGGTTATGCGGCCATTGAGATGCCACATAATGTCCTCAAAGTGATTTCATCAAA
AAGAGCCAAAGGAGTAGTCGCACCGCTCCCAAAAGAGGGGGTAACATTCGATGGTGATGAATTGATAAATAGAGTTATTC
CTCTTAGGTACATATCAGCAAAGAAAATAACGCCATTATTGAGGCAACTCAATGATAATACGGAGTCTGGCAGTATTATT
AATTATGACCCATCTAATATTTTGTTAATTACAGGGCGTGCTGCGGTAGTAAACCGTCTGCATTCAATAGTTACTGATCT
TGACCAAGCCGGAGATAATGAAATTGAGCTTTATAAACTCAATTATGCGATTGCTGCTGATGTTGTTAAAATAGTAAATG
AAGCTATAAATCCAATAAATAATTTAAAACAAGAAGTATCAATAGTAGGAAAGGTTATTGCTGATGAAAGAACAAACTCT
ATATTAATCAGCGGTGATACATATATCAGAAAAAAATCCATTCTAATGATAAAAAAACTCGACAAGAGACAAAGTAGCGA
TGGGAATACAAAAGTGGTTTATATGAAATATGCTCAAGCCTCAAAGCTATTAGATGTCTTAAATGGAATAAGCGAAGGTT
TTCATAATGAGAAAAAAACAAAACAATCTAATCAATGGAATCAGAGACCTGTAGCAATAAAGGCATATGATCAGACGAAT
GCATTAGTTATTACGGCAGATCCTGACATGATGCTAGCCTTAGGAGAAGTAATAGAAAAATTAGATATCAGACGTGCACA
GGTTTTGGTTGAGGCTATTATTGTCGAAACTCAAAACGGAGAAGGTATTAACCTTGGTGTTAAATGGGAAAATAAGCGTT
CTGATGATATTAATTTTATTAAAAATTCCGATGGTTTATTAAATAATAATGGATGGGGCATAGCTACGACAATTACTGGA
TTAACTGCTGGTTTTTATAAAGGAAACTGGGATGTATTGCTTAGCGCTTTATCAACAAATACAAATAATAATATACTCGC
AACTCCCAGTATTGTTACTCTCGATAATATGGAGGCTGAATTCAATGTGGGTCAGGAGGTTCCAGTGCTCATATCAACGC
AAACGACAACCACAGATAAGGTATATAATTCAATTTCAAGGCAATCTATAGGTGTCATGCTAAAAGTAAAGCCACAAATA
AATAAAGGTGATTCGGTTCTACTTGAAATCAGGCAAGAGGTGTCGAGTATCGCTGATAGTTCTACTGTTAATACACATAA
TTTAGGGTCTGTTTTTAATAAACGAGTAGTGAATAATGCAGTATTAGTTAAAAGTGGAGAAACAGTTGTTGTTGGTGGAT
TACTTGATAAGAAGTCTAGTACGATTGTTAATAAAGTCCCCTTCTTGGGTGATCTCCCATTAATAGGATGGCTATTTAGA
CAAACCAAAGAGAAAGTGGAAAAAAGTAATCTTATTCTATTTATAAAACCGACAATACTTAGGGAATCCGATGACTATAG
CGTTGTGACGTCAAAAGAATATAATAAATATAAAAAAGATAATATGAATGGATATACTTTAGCCGATGATATAGTCCTAT
CAGATAAAAAATATTTAAGTGTTATTCCTAGTATTAGGAAAGATATAAATAATTTTTATACACTGTTGGACTCAGAGCTA
TGA

Upstream 100 bases:

>100_bases
ATTCATTAATAATTAACTAATGGGTTATTTAAAATGTTTTTTACTTTATCTGGTCAGAGCGTAGCCTTATCAATCCATCT
AAAATAAATGGTGTAACATC

Downstream 100 bases:

>100_bases
CAGATATTGAGTCTGAGTTCCATACTTTGCCATTAACGTTTAGTTGGTCAAAGAGTCATGGTGTGCTTATTCTGCCTATG
GCACAGGGCAGTCAGTTGAT

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 640; Mature: 640

Protein sequence:

>640_residues
MYISGKGIKSIHGMIFLFTLIMPLDIISANFSVSFKDVDIKEFINSVSKNINKTIIIDPTVQGLISIRSYENLDKDTYYQ
LFLNVLDVYGYAAIEMPHNVLKVISSKRAKGVVAPLPKEGVTFDGDELINRVIPLRYISAKKITPLLRQLNDNTESGSII
NYDPSNILLITGRAAVVNRLHSIVTDLDQAGDNEIELYKLNYAIAADVVKIVNEAINPINNLKQEVSIVGKVIADERTNS
ILISGDTYIRKKSILMIKKLDKRQSSDGNTKVVYMKYAQASKLLDVLNGISEGFHNEKKTKQSNQWNQRPVAIKAYDQTN
ALVITADPDMMLALGEVIEKLDIRRAQVLVEAIIVETQNGEGINLGVKWENKRSDDINFIKNSDGLLNNNGWGIATTITG
LTAGFYKGNWDVLLSALSTNTNNNILATPSIVTLDNMEAEFNVGQEVPVLISTQTTTTDKVYNSISRQSIGVMLKVKPQI
NKGDSVLLEIRQEVSSIADSSTVNTHNLGSVFNKRVVNNAVLVKSGETVVVGGLLDKKSSTIVNKVPFLGDLPLIGWLFR
QTKEKVEKSNLILFIKPTILRESDDYSVVTSKEYNKYKKDNMNGYTLADDIVLSDKKYLSVIPSIRKDINNFYTLLDSEL

Sequences:

>Translated_640_residues
MYISGKGIKSIHGMIFLFTLIMPLDIISANFSVSFKDVDIKEFINSVSKNINKTIIIDPTVQGLISIRSYENLDKDTYYQ
LFLNVLDVYGYAAIEMPHNVLKVISSKRAKGVVAPLPKEGVTFDGDELINRVIPLRYISAKKITPLLRQLNDNTESGSII
NYDPSNILLITGRAAVVNRLHSIVTDLDQAGDNEIELYKLNYAIAADVVKIVNEAINPINNLKQEVSIVGKVIADERTNS
ILISGDTYIRKKSILMIKKLDKRQSSDGNTKVVYMKYAQASKLLDVLNGISEGFHNEKKTKQSNQWNQRPVAIKAYDQTN
ALVITADPDMMLALGEVIEKLDIRRAQVLVEAIIVETQNGEGINLGVKWENKRSDDINFIKNSDGLLNNNGWGIATTITG
LTAGFYKGNWDVLLSALSTNTNNNILATPSIVTLDNMEAEFNVGQEVPVLISTQTTTTDKVYNSISRQSIGVMLKVKPQI
NKGDSVLLEIRQEVSSIADSSTVNTHNLGSVFNKRVVNNAVLVKSGETVVVGGLLDKKSSTIVNKVPFLGDLPLIGWLFR
QTKEKVEKSNLILFIKPTILRESDDYSVVTSKEYNKYKKDNMNGYTLADDIVLSDKKYLSVIPSIRKDINNFYTLLDSEL
>Mature_640_residues
MYISGKGIKSIHGMIFLFTLIMPLDIISANFSVSFKDVDIKEFINSVSKNINKTIIIDPTVQGLISIRSYENLDKDTYYQ
LFLNVLDVYGYAAIEMPHNVLKVISSKRAKGVVAPLPKEGVTFDGDELINRVIPLRYISAKKITPLLRQLNDNTESGSII
NYDPSNILLITGRAAVVNRLHSIVTDLDQAGDNEIELYKLNYAIAADVVKIVNEAINPINNLKQEVSIVGKVIADERTNS
ILISGDTYIRKKSILMIKKLDKRQSSDGNTKVVYMKYAQASKLLDVLNGISEGFHNEKKTKQSNQWNQRPVAIKAYDQTN
ALVITADPDMMLALGEVIEKLDIRRAQVLVEAIIVETQNGEGINLGVKWENKRSDDINFIKNSDGLLNNNGWGIATTITG
LTAGFYKGNWDVLLSALSTNTNNNILATPSIVTLDNMEAEFNVGQEVPVLISTQTTTTDKVYNSISRQSIGVMLKVKPQI
NKGDSVLLEIRQEVSSIADSSTVNTHNLGSVFNKRVVNNAVLVKSGETVVVGGLLDKKSSTIVNKVPFLGDLPLIGWLFR
QTKEKVEKSNLILFIKPTILRESDDYSVVTSKEYNKYKKDNMNGYTLADDIVLSDKKYLSVIPSIRKDINNFYTLLDSEL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=620, Percent_Identity=44.0322580645161, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789793, Length=270, Percent_Identity=27.4074074074074, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 71136; Mature: 71136

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00589 PTS_HPR_SER ; PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYISGKGIKSIHGMIFLFTLIMPLDIISANFSVSFKDVDIKEFINSVSKNINKTIIIDPT
CEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCC
VQGLISIRSYENLDKDTYYQLFLNVLDVYGYAAIEMPHNVLKVISSKRAKGVVAPLPKEG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCCCCCCCC
VTFDGDELINRVIPLRYISAKKITPLLRQLNDNTESGSIINYDPSNILLITGRAAVVNRL
CCCCHHHHHHHHCCHHEECHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHH
HSIVTDLDQAGDNEIELYKLNYAIAADVVKIVNEAINPINNLKQEVSIVGKVIADERTNS
HHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ILISGDTYIRKKSILMIKKLDKRQSSDGNTKVVYMKYAQASKLLDVLNGISEGFHNEKKT
EEEECCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCHHHH
KQSNQWNQRPVAIKAYDQTNALVITADPDMMLALGEVIEKLDIRRAQVLVEAIIVETQNG
HCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
EGINLGVKWENKRSDDINFIKNSDGLLNNNGWGIATTITGLTAGFYKGNWDVLLSALSTN
CEEEEEEEECCCCCCCCCEEECCCCEECCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHCC
TNNNILATPSIVTLDNMEAEFNVGQEVPVLISTQTTTTDKVYNSISRQSIGVMLKVKPQI
CCCCEEECCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCC
NKGDSVLLEIRQEVSSIADSSTVNTHNLGSVFNKRVVNNAVLVKSGETVVVGGLLDKKSS
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCC
TIVNKVPFLGDLPLIGWLFRQTKEKVEKSNLILFIKPTILRESDDYSVVTSKEYNKYKKD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCEEECCCCCEEEECCHHHHHHHC
NMNGYTLADDIVLSDKKYLSVIPSIRKDINNFYTLLDSEL
CCCCEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYISGKGIKSIHGMIFLFTLIMPLDIISANFSVSFKDVDIKEFINSVSKNINKTIIIDPT
CEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEECCC
VQGLISIRSYENLDKDTYYQLFLNVLDVYGYAAIEMPHNVLKVISSKRAKGVVAPLPKEG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHCCCCCCCCCCCCC
VTFDGDELINRVIPLRYISAKKITPLLRQLNDNTESGSIINYDPSNILLITGRAAVVNRL
CCCCHHHHHHHHCCHHEECHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHHHHH
HSIVTDLDQAGDNEIELYKLNYAIAADVVKIVNEAINPINNLKQEVSIVGKVIADERTNS
HHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ILISGDTYIRKKSILMIKKLDKRQSSDGNTKVVYMKYAQASKLLDVLNGISEGFHNEKKT
EEEECCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCHHHH
KQSNQWNQRPVAIKAYDQTNALVITADPDMMLALGEVIEKLDIRRAQVLVEAIIVETQNG
HCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
EGINLGVKWENKRSDDINFIKNSDGLLNNNGWGIATTITGLTAGFYKGNWDVLLSALSTN
CEEEEEEEECCCCCCCCCEEECCCCEECCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHCC
TNNNILATPSIVTLDNMEAEFNVGQEVPVLISTQTTTTDKVYNSISRQSIGVMLKVKPQI
CCCCEEECCCEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCC
NKGDSVLLEIRQEVSSIADSSTVNTHNLGSVFNKRVVNNAVLVKSGETVVVGGLLDKKSS
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCC
TIVNKVPFLGDLPLIGWLFRQTKEKVEKSNLILFIKPTILRESDDYSVVTSKEYNKYKKD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCEEECCCCCEEEECCHHHHHHHC
NMNGYTLADDIVLSDKKYLSVIPSIRKDINNFYTLLDSEL
CCCCEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]