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The map label for this gene is hofF [H]

Identifier: 22127078

GI number: 22127078

Start: 3521012

End: 3522058

Strand: Reverse

Name: hofF [H]

Synonym: y3202

Alternate gene names: 22127078

Gene position: 3522058-3521012 (Counterclockwise)

Preceding gene: 22127079

Following gene: 22127077

Centisome position: 76.55

GC content: 34.48

Gene sequence:

>1047_bases
GTGGTCAAAAAAAATAGTAATAAGACGGATTTGGTATTAATTACACGGCAGATAGCGACATTAGTAAATGCTTCAATGCC
ACTAGATGAGGTTTTAGATATTGTTGGGAAACAAAATAGTAAAAGTAAAATGATTGAAATAATTCAAAGGATTAGAGTTA
ATATACAGGAAGGGCATTCATTCGCTGATGCGCTATCTCCGTTTCCAGCAGTATTTAGCCCATTATACAAAACGATGGTT
ACCGCTGGAGAGGTATCAGGGCATCTGGGATTAGTGCTGGTTCGTCTTGCTGATCATATTGAGCAGACACAAAAAATACA
AAGAAAAATCATTCAGGCACTTATCTACCCTTGTGTGTTGGTTTTAATATCACTAAGTGTCATTATCATATTATTGACAG
CGGTTGTACCGAACATAGTAGAGCAGTTTTCTTTTTCAGAGACGGCGTTACCATTATCAACAAAAGTATTGATGATACTC
AGTTATAGTATAAAAGAAAATGTTATTTTTATCATGGCTATAGGTGTCTCGGCAGTGATTTTTTTAAATAGATTACTAAA
AATAAATAAGATAAATGTCTTTTTTCATAGGCACTATTTAAGTTTGCCAATGCTGGGTAATATGTTTGTTCGTATAAATA
CATCTCGTTATCTTAGAACATTAACAACACTACATTCTAATGGTGTGACAATCGTACAGGCAATGAGTATTAGTAATGCG
GTATTAACGAATGTATATATAAAAAATAAGTTAAACATTTCAGTTAAATTGGTCAGTGAGGGGTGTAGTTTATCGTCATC
TTTAGTTGATAGTGGAGTATTCCCCCCGATCATATTACATATGATCATATCGGGGGAGCGTAGTGGTAAGTTGGACCATA
TGCTAGAAACTGTGGCGGGTGTCCAGGAAGAAGAACTGATGAATCAAATAAGTATAGTAATGTCATTGTTAGAGCCAACC
ATAATTATAGTTATGGCCGCGTTTATTTCATTTGTTATTCTGTCAATTCTTCAGCCAATACTTGAAATTAATAGCCTTGT
CATGTAA

Upstream 100 bases:

>100_bases
ATATTGATGCGGAAAACATACGTATAGCCCGTTATCTCCTTTACAAAAAAAACATGCATGTCTTAAGTATAAAAAAAGAA
TTTTACTTTTTAATAAGTAT

Downstream 100 bases:

>100_bases
TTAATTTTATGAGGGAATAATGGCAAACAAAAAAACAAAAGGATTTACATTGTTGGAAATTATGGTGGTTATTGTTATTC
TGGGCCTACTTGCTAGCTTA

Product: general protein secretion protein

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MVKKNSNKTDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPFPAVFSPLYKTMV
TAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVLVLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMIL
SYSIKENVIFIMAIGVSAVIFLNRLLKINKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNA
VLTNVYIKNKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGKLDHMLETVAGVQEEELMNQISIVMSLLEPT
IIIVMAAFISFVILSILQPILEINSLVM

Sequences:

>Translated_348_residues
MVKKNSNKTDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPFPAVFSPLYKTMV
TAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVLVLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMIL
SYSIKENVIFIMAIGVSAVIFLNRLLKINKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNA
VLTNVYIKNKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGKLDHMLETVAGVQEEELMNQISIVMSLLEPT
IIIVMAAFISFVILSILQPILEINSLVM
>Mature_348_residues
MVKKNSNKTDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPFPAVFSPLYKTMV
TAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVLVLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMIL
SYSIKENVIFIMAIGVSAVIFLNRLLKINKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNA
VLTNVYIKNKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGKLDHMLETVAGVQEEELMNQISIVMSLLEPT
IIIVMAAFISFVILSILQPILEINSLVM

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=345, Percent_Identity=40.8695652173913, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1786295, Length=332, Percent_Identity=24.6987951807229, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 38519; Mature: 38519

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKNSNKTDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHS
CCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
FADALSPFPAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVL
HHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
FLNRLLKINKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNA
HHHHHHHHHHHEEEEEHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHH
VLTNVYIKNKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGKLDHMLETVAG
HHHHHEEECCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
VQEEELMNQISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKKNSNKTDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHS
CCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
FADALSPFPAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVL
HHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
FLNRLLKINKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNA
HHHHHHHHHHHEEEEEHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHH
VLTNVYIKNKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGKLDHMLETVAG
HHHHHEEECCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHC
VQEEELMNQISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]