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The map label for this gene is eco [H]

Identifier: 22126854

GI number: 22126854

Start: 3282323

End: 3282883

Strand: Direct

Name: eco [H]

Synonym: y2977

Alternate gene names: 22126854

Gene position: 3282323-3282883 (Clockwise)

Preceding gene: 22126853

Following gene: 22126856

Centisome position: 71.34

GC content: 45.45

Gene sequence:

>561_bases
TTGAAATCGAGTTTTAGAGACGTATTGCTGATAAAACTTAGAGAAAAAAAGATGAAAAAATGCTCAATCATATTAGCCAG
TGTATTACTAGCGACATCAATCAATGCCATTGCTGATACGCCTACGCCTCTCAATCAGCAGCAGCCATTAGAAAAAATCG
CACCTTATCCACAAGCTGAAAAAGGGATGAGCCGACAGGTCATTTTTCTTGAGCCTCAAAAAGATGAAAGCCGCTTTAAA
GTTGAGCTGCTGATCGGTAAAACTCTCAACGTTGACTGTAACCGCCACATGCTCGGCGGCAATCTGGAAACCAGAACATT
ATCTGGTTGGGGATTCGATTATCTGGTGATGGATAAAATCTCACAACCCGCCTCAACCATGATGGCTTGCCCTGAAGACA
GTAAACCGCAAGTGAAATTTGTTACCGCCAATTTGGGCGACGCGGCAATGCAGCGTTACAACAGCCGCCTACCCATTGTG
GTCTATGTACCGCAAGGGGTGGAAGTCAAATACCGGATCTGGGAAGCGGGCGAAGATATCAGAAGTGCGCAGGTCAAATA
G

Upstream 100 bases:

>100_bases
ATGCGTTTACTGAAAGGCGAAGTCTGTTATTTGCAGCAGCCACTCGCTTTTATACAGGCCGGAGAGATCCTACCCTGTTG
TTGTCAACCCAAGGGCAACA

Downstream 100 bases:

>100_bases
CACTCATTAACCAGGCGCTATTCCGCGCCTGGGCTAAATATTAACATTCTCTTTTAATTACAAAATCTCAATAATATCCA
ACCAACCATGCTGCCCGCAC

Product: ecotin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKSSFRDVLLIKLREKKMKKCSIILASVLLATSINAIADTPTPLNQQQPLEKIAPYPQAEKGMSRQVIFLEPQKDESRFK
VELLIGKTLNVDCNRHMLGGNLETRTLSGWGFDYLVMDKISQPASTMMACPEDSKPQVKFVTANLGDAAMQRYNSRLPIV
VYVPQGVEVKYRIWEAGEDIRSAQVK

Sequences:

>Translated_186_residues
MKSSFRDVLLIKLREKKMKKCSIILASVLLATSINAIADTPTPLNQQQPLEKIAPYPQAEKGMSRQVIFLEPQKDESRFK
VELLIGKTLNVDCNRHMLGGNLETRTLSGWGFDYLVMDKISQPASTMMACPEDSKPQVKFVTANLGDAAMQRYNSRLPIV
VYVPQGVEVKYRIWEAGEDIRSAQVK
>Mature_186_residues
MKSSFRDVLLIKLREKKMKKCSIILASVLLATSINAIADTPTPLNQQQPLEKIAPYPQAEKGMSRQVIFLEPQKDESRFK
VELLIGKTLNVDCNRHMLGGNLETRTLSGWGFDYLVMDKISQPASTMMACPEDSKPQVKFVTANLGDAAMQRYNSRLPIV
VYVPQGVEVKYRIWEAGEDIRSAQVK

Specific function: General inhibitor of pancreatic serine proteases:inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases [H]

COG id: COG4574

COG function: function code R; Serine protease inhibitor ecotin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protease inhibitor I11 (ecotin) family [H]

Homologues:

Organism=Escherichia coli, GI1788538, Length=164, Percent_Identity=64.6341463414634, Blast_Score=207, Evalue=5e-55,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005658
- InterPro:   IPR023189
- InterPro:   IPR023084 [H]

Pfam domain/function: PF03974 Ecotin [H]

EC number: NA

Molecular weight: Translated: 20945; Mature: 20945

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSFRDVLLIKLREKKMKKCSIILASVLLATSINAIADTPTPLNQQQPLEKIAPYPQAE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCC
KGMSRQVIFLEPQKDESRFKVELLIGKTLNVDCNRHMLGGNLETRTLSGWGFDYLVMDKI
CCCCCEEEEEECCCCCCEEEEEEEECCEEECCCCCEEECCCCCEEEECCCCCCEEEEHHC
SQPASTMMACPEDSKPQVKFVTANLGDAAMQRYNSRLPIVVYVPQGVEVKYRIWEAGEDI
CCCHHHEEECCCCCCCCEEEEEECCCHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCH
RSAQVK
HHCCCC
>Mature Secondary Structure
MKSSFRDVLLIKLREKKMKKCSIILASVLLATSINAIADTPTPLNQQQPLEKIAPYPQAE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCC
KGMSRQVIFLEPQKDESRFKVELLIGKTLNVDCNRHMLGGNLETRTLSGWGFDYLVMDKI
CCCCCEEEEEECCCCCCEEEEEEEECCEEECCCCCEEECCCCCEEEECCCCCCEEEEHHC
SQPASTMMACPEDSKPQVKFVTANLGDAAMQRYNSRLPIVVYVPQGVEVKYRIWEAGEDI
CCCHHHEEECCCCCCCCEEEEEECCCHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCH
RSAQVK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA