Click here to switch to the map view.

The map label for this gene is aroA [H]

Identifier: 22126661

GI number: 22126661

Start: 3076707

End: 3078038

Strand: Reverse

Name: aroA [H]

Synonym: y2783

Alternate gene names: 22126661

Gene position: 3078038-3076707 (Counterclockwise)

Preceding gene: 22126662

Following gene: 22126660

Centisome position: 66.9

GC content: 51.13

Gene sequence:

>1332_bases
TTGTTCGAGGCTGTTGCCTATACAAGACTGGAGAATGTTTTTCCCATGCTGGAATCCCTGACCTTACAACCCATTGCCCT
AGTTAATGGCACCGTTAATTTACCTGGTTCGAAGAGTGTCTCTAACCGCGCACTGCTTCTGGCCGCGTTGGCCGAAGGGA
CCACTCAGTTGAATAACGTGTTAGACAGCGATGACATCCGCCACATGCTCAATGCATTACAGGCATTAGGGGTGGACTTC
CGCCTTTCTGCTGATCGCACATGCTGTGAGGTTGATGGTCTGGGGGGGAAATTAGTGGCTGAACAGCCATTGTCGCTTTT
CTTGGGCAATGCCGGCACAGCCATGCGTCCTTTGGCCGCGGTGTTATGTTTGGGTAATAGCGATATCGTACTGACGGGTG
AGCCTCGGATGAAGGAGCGGCCAATTGGCCATTTGGTGGATGCGCTACGTCAGGGCGGTGCACAGATCGATTATCTGGAA
CAAGAAAATTACCCGCCATTACGTTTACGTGGTGGTTTCCGAGGGGGGGAGTTAACTGTTGATGGGCGTGTCTCTAGCCA
GTTCCTGACTGCTTTATTGATGACCGCCCCGCTGGCGGAGCAAGATACGACTATTCGGATTATGGGTGATCTGGTTTCCA
AACCTTATATCGATATTACTCTGCACTTGATGAAAGCATTTGGTATTGACGTGGGGCATGAGAACTACCAAATTTTCCAC
ATCAAAGGGGGCCAGACCTACCGCTCACCAGGGACTTATTTGGTTGAGGGCGATGCCTCGTCGGCTTCCTACTTCTTAGC
GGCTGCGGCTATTAAGGGGGGAACAGTGCGTGTCACTGGTATTGGCAAGAAAAGTGTACAGGGCGACACTAAATTTGCCG
ATGTGTTGGAAAAAATGGGCGCGAAAGTGACGTGGGGGGATGATTATATCGAGTGCAGTCGTGGTGAATTACAGGGCATT
GACATGGATATGAACCACATTCCTGATGCTGCAATGACCATTGCGACTACGGCATTATTTGCCACGGGCCCAACGACGAT
CCGCAATATCTACAACTGGCGGGTAAAAGAAACTGACCGGCTGACGGCGATGGCAACCGAGTTGAGAAAAGTAGGTGCTG
AAGTGGAAGAGGGGGAAGATTACATCCGCGTGGTTCCACCCTTGCAGCTAACTGCTGCAGATATTGGTACCTACGATGAC
CACCGTATGGCGATGTGTTTCTCGCTGGTCGCGTTATCAGATACCCCCGTGACGATCCTTGACCCGAAATGTACCGCAAA
AACCTTCCCTGATTATTTTGAACAGTTTGCGCGTCTGAGCCAACTGGCCTGA

Upstream 100 bases:

>100_bases
CTCATCCGCTCCGGATCGATGGGCCTCTTGCAACAATGAGTTCGTGTGTAATAACGCGTAAATTCGTAATAACACGTTAA
TTTGTAATAACAGCTTCGTT

Downstream 100 bases:

>100_bases
TAGCACCTGAATTGAATGGGTTCTATTACACTAAAACGGGCGCGAATCAAGCGCCTATTTCTATAGGATATCTCTCTTCA
TTGTTCATTTTTTTTATAAA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MFEAVAYTRLENVFPMLESLTLQPIALVNGTVNLPGSKSVSNRALLLAALAEGTTQLNNVLDSDDIRHMLNALQALGVDF
RLSADRTCCEVDGLGGKLVAEQPLSLFLGNAGTAMRPLAAVLCLGNSDIVLTGEPRMKERPIGHLVDALRQGGAQIDYLE
QENYPPLRLRGGFRGGELTVDGRVSSQFLTALLMTAPLAEQDTTIRIMGDLVSKPYIDITLHLMKAFGIDVGHENYQIFH
IKGGQTYRSPGTYLVEGDASSASYFLAAAAIKGGTVRVTGIGKKSVQGDTKFADVLEKMGAKVTWGDDYIECSRGELQGI
DMDMNHIPDAAMTIATTALFATGPTTIRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDYIRVVPPLQLTAADIGTYDD
HRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQFARLSQLA

Sequences:

>Translated_443_residues
MFEAVAYTRLENVFPMLESLTLQPIALVNGTVNLPGSKSVSNRALLLAALAEGTTQLNNVLDSDDIRHMLNALQALGVDF
RLSADRTCCEVDGLGGKLVAEQPLSLFLGNAGTAMRPLAAVLCLGNSDIVLTGEPRMKERPIGHLVDALRQGGAQIDYLE
QENYPPLRLRGGFRGGELTVDGRVSSQFLTALLMTAPLAEQDTTIRIMGDLVSKPYIDITLHLMKAFGIDVGHENYQIFH
IKGGQTYRSPGTYLVEGDASSASYFLAAAAIKGGTVRVTGIGKKSVQGDTKFADVLEKMGAKVTWGDDYIECSRGELQGI
DMDMNHIPDAAMTIATTALFATGPTTIRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDYIRVVPPLQLTAADIGTYDD
HRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQFARLSQLA
>Mature_443_residues
MFEAVAYTRLENVFPMLESLTLQPIALVNGTVNLPGSKSVSNRALLLAALAEGTTQLNNVLDSDDIRHMLNALQALGVDF
RLSADRTCCEVDGLGGKLVAEQPLSLFLGNAGTAMRPLAAVLCLGNSDIVLTGEPRMKERPIGHLVDALRQGGAQIDYLE
QENYPPLRLRGGFRGGELTVDGRVSSQFLTALLMTAPLAEQDTTIRIMGDLVSKPYIDITLHLMKAFGIDVGHENYQIFH
IKGGQTYRSPGTYLVEGDASSASYFLAAAAIKGGTVRVTGIGKKSVQGDTKFADVLEKMGAKVTWGDDYIECSRGELQGI
DMDMNHIPDAAMTIATTALFATGPTTIRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDYIRVVPPLQLTAADIGTYDD
HRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQFARLSQLA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=427, Percent_Identity=80.327868852459, Blast_Score=695, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320332, Length=444, Percent_Identity=40.7657657657658, Blast_Score=271, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 48105; Mature: 48105

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEAVAYTRLENVFPMLESLTLQPIALVNGTVNLPGSKSVSNRALLLAALAEGTTQLNNV
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCEEEEEHHHHCHHHHHHH
LDSDDIRHMLNALQALGVDFRLSADRTCCEVDGLGGKLVAEQPLSLFLGNAGTAMRPLAA
CCCHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCEEECCCCHHEEECCCCCHHHHHHH
VLCLGNSDIVLTGEPRMKERPIGHLVDALRQGGAQIDYLEQENYPPLRLRGGFRGGELTV
HEEECCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCEEEE
DGRVSSQFLTALLMTAPLAEQDTTIRIMGDLVSKPYIDITLHLMKAFGIDVGHENYQIFH
CCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEE
IKGGQTYRSPGTYLVEGDASSASYFLAAAAIKGGTVRVTGIGKKSVQGDTKFADVLEKMG
EECCCEECCCCCEEEECCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHC
AKVTWGDDYIECSRGELQGIDMDMNHIPDAAMTIATTALFATGPTTIRNIYNWRVKETDR
CEEEECCCCEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCEECCCHH
LTAMATELRKVGAEVEEGEDYIRVVPPLQLTAADIGTYDDHRMAMCFSLVALSDTPVTIL
HHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEHHCCCCCCHHHHHHHHHHHCCCCCEEEE
DPKCTAKTFPDYFEQFARLSQLA
CCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFEAVAYTRLENVFPMLESLTLQPIALVNGTVNLPGSKSVSNRALLLAALAEGTTQLNNV
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCEEEEEHHHHCHHHHHHH
LDSDDIRHMLNALQALGVDFRLSADRTCCEVDGLGGKLVAEQPLSLFLGNAGTAMRPLAA
CCCHHHHHHHHHHHHHCCCEEECCCCCEEEECCCCCEEECCCCHHEEECCCCCHHHHHHH
VLCLGNSDIVLTGEPRMKERPIGHLVDALRQGGAQIDYLEQENYPPLRLRGGFRGGELTV
HEEECCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCEEEE
DGRVSSQFLTALLMTAPLAEQDTTIRIMGDLVSKPYIDITLHLMKAFGIDVGHENYQIFH
CCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEE
IKGGQTYRSPGTYLVEGDASSASYFLAAAAIKGGTVRVTGIGKKSVQGDTKFADVLEKMG
EECCCEECCCCCEEEECCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHC
AKVTWGDDYIECSRGELQGIDMDMNHIPDAAMTIATTALFATGPTTIRNIYNWRVKETDR
CEEEECCCCEECCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCEECCCHH
LTAMATELRKVGAEVEEGEDYIRVVPPLQLTAADIGTYDDHRMAMCFSLVALSDTPVTIL
HHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEHHCCCCCCHHHHHHHHHHHCCCCCEEEE
DPKCTAKTFPDYFEQFARLSQLA
CCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2691337 [H]