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The map label for this gene is ybtD [H]

Identifier: 22126465

GI number: 22126465

Start: 2846453

End: 2847109

Strand: Reverse

Name: ybtD [H]

Synonym: y2584

Alternate gene names: 22126465

Gene position: 2847109-2846453 (Counterclockwise)

Preceding gene: 22126470

Following gene: 22126464

Centisome position: 61.88

GC content: 46.12

Gene sequence:

>657_bases
TTGGCCAAGTGTGATTTTGAGGTGAATGAATATCGGGACGAGTTGTTTGCAGCCTATGGCATTCCGTTTCCTGGGAGTCT
GAACAAGGCGGTGATCAAACGTCGTGCCGAATACCTGGCTGGGCGTTTTGTGGCTCGGCAGGTGTTGAACTTATTGGATA
TCCGCGATTATCCGCTAGCGACGGGAATGGATCGTGCGCCGCAGTGGCCAACCAACCTGATTGGCAGTATTAGCCATAAT
AACCAACGTGCGTTATGTGCCGCCCAAATGATAGAGCCAAGGGGCGTTGAGTCTTCTACTTTGCATGGCATAGGTTTGGA
TATTGAAAGCCATATCGCTGAGGAAAAAGCGCAAGAGATTTGGTCGGGTATTATCAGCGATGAGGAGTATTCGCTATTAC
AACAAGGCCCATTGCCCTTTAACCAAGCACTGACATTGGTTTTTTCGGCTAAAGAAAGTTTATTTAAAGCGGTCTATCCC
CAATCTGGCCGCTATTTCGATTTTATCGAAGCACGTCTGCTGAGTTACTCATTGGTGTCAGGCAATTTTGAATTGCAGCT
ATTACGACATTTAAATGATGAATTTCCCGCTGGCCGTTGTTTTAGTGGCTGTTTTAGTCTCAGTGAGACGGATCTTCAGA
CCTTTATTGCTTATTAA

Upstream 100 bases:

>100_bases
ATAATTAGGGTAACGCGTGACGTCGGTATTCATCCGTAATTTTACTTTTGCTGCACTGCCAGCCAACGGCCTCAATGGTC
AACCCCCGTTTCACGGGCTT

Downstream 100 bases:

>100_bases
AACCAAGAATTATTTATCTGAGAATAAGGGGCTGAATATGAAAGAAGTCTTAACCATCAGCGTGTTGGAAAAATCGTTAA
AGGACATCACTCGTTATGCA

Product: yersiniabactin biosynthesis component

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MAKCDFEVNEYRDELFAAYGIPFPGSLNKAVIKRRAEYLAGRFVARQVLNLLDIRDYPLATGMDRAPQWPTNLIGSISHN
NQRALCAAQMIEPRGVESSTLHGIGLDIESHIAEEKAQEIWSGIISDEEYSLLQQGPLPFNQALTLVFSAKESLFKAVYP
QSGRYFDFIEARLLSYSLVSGNFELQLLRHLNDEFPAGRCFSGCFSLSETDLQTFIAY

Sequences:

>Translated_218_residues
MAKCDFEVNEYRDELFAAYGIPFPGSLNKAVIKRRAEYLAGRFVARQVLNLLDIRDYPLATGMDRAPQWPTNLIGSISHN
NQRALCAAQMIEPRGVESSTLHGIGLDIESHIAEEKAQEIWSGIISDEEYSLLQQGPLPFNQALTLVFSAKESLFKAVYP
QSGRYFDFIEARLLSYSLVSGNFELQLLRHLNDEFPAGRCFSGCFSLSETDLQTFIAY
>Mature_217_residues
AKCDFEVNEYRDELFAAYGIPFPGSLNKAVIKRRAEYLAGRFVARQVLNLLDIRDYPLATGMDRAPQWPTNLIGSISHNN
QRALCAAQMIEPRGVESSTLHGIGLDIESHIAEEKAQEIWSGIISDEEYSLLQQGPLPFNQALTLVFSAKESLFKAVYPQ
SGRYFDFIEARLLSYSLVSGNFELQLLRHLNDEFPAGRCFSGCFSLSETDLQTFIAY

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=175, Percent_Identity=34.8571428571429, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 24541; Mature: 24410

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKCDFEVNEYRDELFAAYGIPFPGSLNKAVIKRRAEYLAGRFVARQVLNLLDIRDYPLA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TGMDRAPQWPTNLIGSISHNNQRALCAAQMIEPRGVESSTLHGIGLDIESHIAEEKAQEI
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
WSGIISDEEYSLLQQGPLPFNQALTLVFSAKESLFKAVYPQSGRYFDFIEARLLSYSLVS
HHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GNFELQLLRHLNDEFPAGRCFSGCFSLSETDLQTFIAY
CCHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
AKCDFEVNEYRDELFAAYGIPFPGSLNKAVIKRRAEYLAGRFVARQVLNLLDIRDYPLA
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TGMDRAPQWPTNLIGSISHNNQRALCAAQMIEPRGVESSTLHGIGLDIESHIAEEKAQEI
CCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
WSGIISDEEYSLLQQGPLPFNQALTLVFSAKESLFKAVYPQSGRYFDFIEARLLSYSLVS
HHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GNFELQLLRHLNDEFPAGRCFSGCFSLSETDLQTFIAY
CCHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]