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The map label for this gene is hpcH [H]

Identifier: 22126423

GI number: 22126423

Start: 2804046

End: 2804870

Strand: Reverse

Name: hpcH [H]

Synonym: y2541

Alternate gene names: 22126423

Gene position: 2804870-2804046 (Counterclockwise)

Preceding gene: 22126424

Following gene: 22126422

Centisome position: 60.97

GC content: 51.15

Gene sequence:

>825_bases
TTGTTTAGGGGCTTAGTGATGTTTCCATTAACAAATAAATTTAAACAGGCACTGAAAGCAAAAAAACCACAAATTGGCTT
ATGGCTAGGTTTGTGTAGCAATTACAGTGCTGAAATCCTGGCCGGTGCGGGGTTTGACTGGTTGTTGATCGACGATGAAC
ATGCCCCGAATGATGTACAACGGATCTTAGGGCAATTACAGACGATCGCGTCTTATCCGAGCCAGCCCGTGGTTCGTCCG
GCGTGGAATGACCCGGTAATGATAAAAAAATTGCTGGATATTGGCGCACAAACCTTATTGCTCCCAATGATTCAAAACGC
CGCACAAGCGCGCGCTGCGATCAGTGCGACCCGCTATCCTCCGCAGGGGATTCGCGGCGTCGGAAGCGCGCTGGCCCGAG
CTTCCCGTTGGAATAGAATCGCAGATTATGTGCAGCAAGCTGACGAACAGATATGCGTATTGCTCCAAATAGAAACACGC
GAAGCACTGCGCAATCTACCCGAGATTTTGGCGGTTGATGGTGTCGATGGTGTGTTTATCGGCCCGGCCGATCTCGCTTC
TGATATGGGTTTTGCGGGTAATCCACACCATCCCGAGGTCCGTCAAGCCATCGATAGCGCAGTGATGAAAATTAAGACTG
CCGATAAAGCGGCCGGGATCTTGATGACCACGCCAGAGTTGGCTGAGTATTATCTGAAACTGGGGGCACTATTTGTGGCT
GTAGGTGTAGATACGACGCTATTAGCACGCACAGCCGAGGCACTGGCAGCCCGCTTCGGTGTGGGTAATCATCATCCACC
GCTTGCACCATCGGGTGGATATTAA

Upstream 100 bases:

>100_bases
CAGATAATTCTTGGTGGATCCTTCACCCGCCCGGTTCCAGCGCGTCGTGGTGATACTTTCCATGTGGATTATGGCCCGTT
AGGTGCCATAAGTTGCCACT

Downstream 100 bases:

>100_bases
CCCTGTCCGTATCGAGGACATTATCATGAGCGATTCACTGTCTACCGCACCGGGCGTTACCCCGCCGGTCAATAAAAACA
CACCATTAAGTGCGCAGCAG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MFRGLVMFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQLQTIASYPSQPVVRP
AWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETR
EALRNLPEILAVDGVDGVFIGPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVA
VGVDTTLLARTAEALAARFGVGNHHPPLAPSGGY

Sequences:

>Translated_274_residues
MFRGLVMFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQLQTIASYPSQPVVRP
AWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETR
EALRNLPEILAVDGVDGVFIGPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVA
VGVDTTLLARTAEALAARFGVGNHHPPLAPSGGY
>Mature_274_residues
MFRGLVMFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQRILGQLQTIASYPSQPVVRP
AWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYPPQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETR
EALRNLPEILAVDGVDGVFIGPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVA
VGVDTTLLARTAEALAARFGVGNHHPPLAPSGGY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=262, Percent_Identity=51.1450381679389, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1789514, Length=253, Percent_Identity=44.6640316205534, Blast_Score=221, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 29558; Mature: 29558

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGLVMFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQ
CCCCEEECCCCHHHHHHHHHCCCCCEEEEEHHCCCCCCEEECCCCEEEEECCCCCCHHHH
RILGQLQTIASYPSQPVVRPAWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYP
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
PQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETREALRNLPEILAVDGVDGVFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHCCHHHEEECCCCCEEE
GPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVA
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHH
VGVDTTLLARTAEALAARFGVGNHHPPLAPSGGY
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFRGLVMFPLTNKFKQALKAKKPQIGLWLGLCSNYSAEILAGAGFDWLLIDDEHAPNDVQ
CCCCEEECCCCHHHHHHHHHCCCCCEEEEEHHCCCCCCEEECCCCEEEEECCCCCCHHHH
RILGQLQTIASYPSQPVVRPAWNDPVMIKKLLDIGAQTLLLPMIQNAAQARAAISATRYP
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
PQGIRGVGSALARASRWNRIADYVQQADEQICVLLQIETREALRNLPEILAVDGVDGVFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHCCHHHEEECCCCCEEE
GPADLASDMGFAGNPHHPEVRQAIDSAVMKIKTADKAAGILMTTPELAEYYLKLGALFVA
CHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHH
VGVDTTLLARTAEALAARFGVGNHHPPLAPSGGY
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA