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The map label for this gene is insF1 [H]

Identifier: 22126320

GI number: 22126320

Start: 2697266

End: 2698225

Strand: Direct

Name: insF1 [H]

Synonym: y2436

Alternate gene names: 22126320

Gene position: 2697266-2698225 (Clockwise)

Preceding gene: 22126319

Following gene: 22126321

Centisome position: 58.63

GC content: 57.08

Gene sequence:

>960_bases
GTGGCCTGTCGCGAAGTTGAGCTCAGCGTACGGACCTGGCAACGGTGGCAAACCTCCCCGGAAGACCAAAGAACTACGGC
GATACGCCCGGAGCCCACCAATCGCCTGAGAGTGGAAGAAGAGCAGCAGATACGGGCGGTCTGCCATCAACCCGAGTATG
CCAACCTGCCGCCGTCGCAAATCGTGCCACGGTTGGCGGATAAAGGGGTTTATCTGGCGAGTGAATCGACCTTTTATCGG
GTGCTGCGCCGTCATGGAGAAGTACATCACCGTGGCCGTTGCCTCAAGCCGGGCCGGGTAAAACCGCCAACAACGTTCAC
CGCCTCCGGGCCTTGTCAGGTGTGGACGTGGGACATCACGTGGCTGCCTTCATGGGTACGGGGTCGTTGGTATTATCTGT
ACCTGGTGGAGGATGTGTTCAGCCGTAAAATCACCGGAGAAGAAGTCCATGAAACCGAGAGTGGTGAGTTGGCGGCGGCG
CTGATGCAACGTACGGTACTGCGGGAGCGCTGTTATCGCCAGCCACTGGTGCTGCATGCAGATAATGGTGCGGCGATGAA
GTCGCAGACCCTGCAGGTGAAGCTGGCAGAACTGAATATCTCGCCTTCACACAGCCGACCGCGTGTGAGCAATGATAATG
CGTATGTGGAGTCGTTGTTCCGCACGCTGAAATATGTGCCTCAGTGGCCATCCTCAGGCTTTAACCATCTTGAGGAAGCG
CGGGTCTGGGTGGATAAATTTACCCGTTGGTATAACGAAGAGCACCGGCACAGTGGCATAGGTTATGTGACGCCGTTGCA
GCGACATACGGGTGAGGACAAGGCGCTGTTGGCCCAACGAGATAAGGTCTATCAAGCGGCGAGAGCCGCGAACCCCAAAC
GTTGGTCACGACAGACGCGAAACTGGGAATGGCAGGAAAGCGTGACATTAAATCCAGAGCGGGGAAAACAGGCCGCATAA

Upstream 100 bases:

>100_bases
ACTACGGGGAAACGGACGAGGACGACTGACACCCCGAGAGGAACGCCAGCAGTTTATCGCGTGGATAAATGAGGCCGTCG
CTGCTGGTGCACGCCGGGCG

Downstream 100 bases:

>100_bases
ATCAACCTGGGGTGACAACTACCTTGACACTTACCGCCAACGCACATGTAGTATGACAGCCATACAAGCAAGAAATATCT
TAAAATTTCATTTTTGAGTT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MACREVELSVRTWQRWQTSPEDQRTTAIRPEPTNRLRVEEEQQIRAVCHQPEYANLPPSQIVPRLADKGVYLASESTFYR
VLRRHGEVHHRGRCLKPGRVKPPTTFTASGPCQVWTWDITWLPSWVRGRWYYLYLVEDVFSRKITGEEVHETESGELAAA
LMQRTVLRERCYRQPLVLHADNGAAMKSQTLQVKLAELNISPSHSRPRVSNDNAYVESLFRTLKYVPQWPSSGFNHLEEA
RVWVDKFTRWYNEEHRHSGIGYVTPLQRHTGEDKALLAQRDKVYQAARAANPKRWSRQTRNWEWQESVTLNPERGKQAA

Sequences:

>Translated_319_residues
MACREVELSVRTWQRWQTSPEDQRTTAIRPEPTNRLRVEEEQQIRAVCHQPEYANLPPSQIVPRLADKGVYLASESTFYR
VLRRHGEVHHRGRCLKPGRVKPPTTFTASGPCQVWTWDITWLPSWVRGRWYYLYLVEDVFSRKITGEEVHETESGELAAA
LMQRTVLRERCYRQPLVLHADNGAAMKSQTLQVKLAELNISPSHSRPRVSNDNAYVESLFRTLKYVPQWPSSGFNHLEEA
RVWVDKFTRWYNEEHRHSGIGYVTPLQRHTGEDKALLAQRDKVYQAARAANPKRWSRQTRNWEWQESVTLNPERGKQAA
>Mature_318_residues
ACREVELSVRTWQRWQTSPEDQRTTAIRPEPTNRLRVEEEQQIRAVCHQPEYANLPPSQIVPRLADKGVYLASESTFYRV
LRRHGEVHHRGRCLKPGRVKPPTTFTASGPCQVWTWDITWLPSWVRGRWYYLYLVEDVFSRKITGEEVHETESGELAAAL
MQRTVLRERCYRQPLVLHADNGAAMKSQTLQVKLAELNISPSHSRPRVSNDNAYVESLFRTLKYVPQWPSSGFNHLEEAR
VWVDKFTRWYNEEHRHSGIGYVTPLQRHTGEDKALLAQRDKVYQAARAANPKRWSRQTRNWEWQESVTLNPERGKQAA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=250, Percent_Identity=30.4, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787263, Length=250, Percent_Identity=30.4, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786753, Length=250, Percent_Identity=30.4, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786570, Length=250, Percent_Identity=30.4, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786490, Length=250, Percent_Identity=30.4, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 37325; Mature: 37194

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACREVELSVRTWQRWQTSPEDQRTTAIRPEPTNRLRVEEEQQIRAVCHQPEYANLPPSQ
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
IVPRLADKGVYLASESTFYRVLRRHGEVHHRGRCLKPGRVKPPTTFTASGPCQVWTWDIT
HHHHHHCCCEEEECCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEECCCCCEEEEEEEE
WLPSWVRGRWYYLYLVEDVFSRKITGEEVHETESGELAAALMQRTVLRERCYRQPLVLHA
ECCHHHCCCEEEEEEEHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
DNGAAMKSQTLQVKLAELNISPSHSRPRVSNDNAYVESLFRTLKYVPQWPSSGFNHLEEA
CCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RVWVDKFTRWYNEEHRHSGIGYVTPLQRHTGEDKALLAQRDKVYQAARAANPKRWSRQTR
HHHHHHHHHHHCHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCC
NWEWQESVTLNPERGKQAA
CCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
ACREVELSVRTWQRWQTSPEDQRTTAIRPEPTNRLRVEEEQQIRAVCHQPEYANLPPSQ
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHH
IVPRLADKGVYLASESTFYRVLRRHGEVHHRGRCLKPGRVKPPTTFTASGPCQVWTWDIT
HHHHHHCCCEEEECCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEECCCCCEEEEEEEE
WLPSWVRGRWYYLYLVEDVFSRKITGEEVHETESGELAAALMQRTVLRERCYRQPLVLHA
ECCHHHCCCEEEEEEEHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
DNGAAMKSQTLQVKLAELNISPSHSRPRVSNDNAYVESLFRTLKYVPQWPSSGFNHLEEA
CCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RVWVDKFTRWYNEEHRHSGIGYVTPLQRHTGEDKALLAQRDKVYQAARAANPKRWSRQTR
HHHHHHHHHHHCHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCC
NWEWQESVTLNPERGKQAA
CCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]