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The map label for this gene is ynfL [H]

Identifier: 22126266

GI number: 22126266

Start: 2629576

End: 2630472

Strand: Reverse

Name: ynfL [H]

Synonym: y2382

Alternate gene names: 22126266

Gene position: 2630472-2629576 (Counterclockwise)

Preceding gene: 22126278

Following gene: 22126261

Centisome position: 57.17

GC content: 50.84

Gene sequence:

>897_bases
TTGCTTTTGGATATGAAGATGGAGTTACGTCATATACGCTATTTTCTGGCTGTTGCTGAAGAACGACATTTCACACGCGC
TGCCGCCCGGCTAGGAATAGGACAGCCTCCTCTCAGTCAGCAAATTAAGGATCTGGAGAGGGAACTCGGTGCGTTGTTGT
TCCGGCGGGTTTCTTATGGCGCAGAATTAACTGAGGCAGGTATTGCATTTCTGGATGTTGTCAAAGAGATACCTGTGATG
GCAGAAAGAGCGGCCCAAGCTGCGCAGCGGGCTGTCAGGGGAGAACTCGGCGTACTCAGAGTTGGCTTTACCGCGTCATC
AGCATTTAACAGCGTAGTGCCTACGGCGATCCGTGCCTTTAGACGTGCATATCCTGATGTACGATTACAGCTAGAGGAAG
ATAACACGACTCGACTGGCTGATGGATTGAATGAAGGCTCGCTCGATGTCGCTTTCCTGAGGCCAGGCTTTGCTGGCAGC
GAACGTTTCCACCTCAGAATGCTATCTGAAGAGCCGATGATGATTGTCATGGCGGAAAATCACCCGGCGGCATCGTATGA
AGAAATTTCGCTTAGCGCATTCAGGGATGAAACTTTCCTGCTATTTCCACGAGAGATTGGTCTGACGCTTTACGATTCAG
TGATTGAGTCATGCCGTACTGCGGGTTTTGAGCCGACTATTGGCCAACTGGCACCACAAATCGCTTCGGTCATTAATCTC
GTTGCGGCAGAAATGGGCGTTTCAATCGTTCCGGCTTCCATGAGCCAAGTGAAAGTTATTGGGGTTGTATATCGACACAT
TGCTGACCAGACGCCGACAGCAAAGCTCGCGTTGGCGTACCGGCGAGGGGATACCTCGCCGGTATTAAGAAACTTTGTCC
TGACCGTGTTTCCTTGA

Upstream 100 bases:

>100_bases
TTGGTAAGTATCAAGATTAGACATGATGTTCCTCCTCCAACTTCGCTTAGTCTATGGTCGCCGTTATCATTTATCCAATA
TATGATACCGGTGTGTTTAA

Downstream 100 bases:

>100_bases
ATTCTCTCCGTAGGTTAAGGCGACTCTATAGGTTAAAACGATCCAATCGGTTAAAAAACGCCCCAAGAGCAAAATTATTG
GCTATCAATATCCGGCCTGC

Product: transcriptional regulator LYSR-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLLDMKMELRHIRYFLAVAEERHFTRAAARLGIGQPPLSQQIKDLERELGALLFRRVSYGAELTEAGIAFLDVVKEIPVM
AERAAQAAQRAVRGELGVLRVGFTASSAFNSVVPTAIRAFRRAYPDVRLQLEEDNTTRLADGLNEGSLDVAFLRPGFAGS
ERFHLRMLSEEPMMIVMAENHPAASYEEISLSAFRDETFLLFPREIGLTLYDSVIESCRTAGFEPTIGQLAPQIASVINL
VAAEMGVSIVPASMSQVKVIGVVYRHIADQTPTAKLALAYRRGDTSPVLRNFVLTVFP

Sequences:

>Translated_298_residues
MLLDMKMELRHIRYFLAVAEERHFTRAAARLGIGQPPLSQQIKDLERELGALLFRRVSYGAELTEAGIAFLDVVKEIPVM
AERAAQAAQRAVRGELGVLRVGFTASSAFNSVVPTAIRAFRRAYPDVRLQLEEDNTTRLADGLNEGSLDVAFLRPGFAGS
ERFHLRMLSEEPMMIVMAENHPAASYEEISLSAFRDETFLLFPREIGLTLYDSVIESCRTAGFEPTIGQLAPQIASVINL
VAAEMGVSIVPASMSQVKVIGVVYRHIADQTPTAKLALAYRRGDTSPVLRNFVLTVFP
>Mature_298_residues
MLLDMKMELRHIRYFLAVAEERHFTRAAARLGIGQPPLSQQIKDLERELGALLFRRVSYGAELTEAGIAFLDVVKEIPVM
AERAAQAAQRAVRGELGVLRVGFTASSAFNSVVPTAIRAFRRAYPDVRLQLEEDNTTRLADGLNEGSLDVAFLRPGFAGS
ERFHLRMLSEEPMMIVMAENHPAASYEEISLSAFRDETFLLFPREIGLTLYDSVIESCRTAGFEPTIGQLAPQIASVINL
VAAEMGVSIVPASMSQVKVIGVVYRHIADQTPTAKLALAYRRGDTSPVLRNFVLTVFP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=40.8304498269896, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=34.1379310344828, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI2367136, Length=284, Percent_Identity=30.9859154929577, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790399, Length=283, Percent_Identity=31.8021201413428, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI157672245, Length=250, Percent_Identity=32.4, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787601, Length=301, Percent_Identity=27.2425249169435, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081904, Length=194, Percent_Identity=29.8969072164948, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=25.7142857142857, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=23.6, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=26.5873015873016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=24.3027888446215, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789173, Length=138, Percent_Identity=37.6811594202899, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=25.1677852348993, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693105, Length=191, Percent_Identity=25.6544502617801, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=174, Percent_Identity=29.8850574712644, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32925; Mature: 32925

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDMKMELRHIRYFLAVAEERHFTRAAARLGIGQPPLSQQIKDLERELGALLFRRVSYG
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AELTEAGIAFLDVVKEIPVMAERAAQAAQRAVRGELGVLRVGFTASSAFNSVVPTAIRAF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHH
RRAYPDVRLQLEEDNTTRLADGLNEGSLDVAFLRPGFAGSERFHLRMLSEEPMMIVMAEN
HHCCCCEEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEECC
HPAASYEEISLSAFRDETFLLFPREIGLTLYDSVIESCRTAGFEPTIGQLAPQIASVINL
CCCCCHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VAAEMGVSIVPASMSQVKVIGVVYRHIADQTPTAKLALAYRRGDTSPVLRNFVLTVFP
HHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCCHHHHHHHHEECC
>Mature Secondary Structure
MLLDMKMELRHIRYFLAVAEERHFTRAAARLGIGQPPLSQQIKDLERELGALLFRRVSYG
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AELTEAGIAFLDVVKEIPVMAERAAQAAQRAVRGELGVLRVGFTASSAFNSVVPTAIRAF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHH
RRAYPDVRLQLEEDNTTRLADGLNEGSLDVAFLRPGFAGSERFHLRMLSEEPMMIVMAEN
HHCCCCEEEEECCCCCHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEECC
HPAASYEEISLSAFRDETFLLFPREIGLTLYDSVIESCRTAGFEPTIGQLAPQIASVINL
CCCCCHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
VAAEMGVSIVPASMSQVKVIGVVYRHIADQTPTAKLALAYRRGDTSPVLRNFVLTVFP
HHHHHCCEEECCCHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]