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The map label for this gene is narX [H]

Identifier: 22126235

GI number: 22126235

Start: 2590748

End: 2592553

Strand: Direct

Name: narX [H]

Synonym: y2351

Alternate gene names: 22126235

Gene position: 2590748-2592553 (Clockwise)

Preceding gene: 22126226

Following gene: 22126236

Centisome position: 56.31

GC content: 47.01

Gene sequence:

>1806_bases
TTGGTCAAAGGAATCTCCCACGCGATGAAACGTTATCTGACCCCATTGGTTAATCAGATTGCTTTGTTGATGATACTGCT
GGGCATGCTAGGGCTGGCGGGTATGGTTATTTCATCATGGATGGCACAAAGCATTCAGGGCAATGCCCACGCCATCAACC
AAGCAGGCTCATTGCGTATGCAGAGCTACAGGTTACTTTCAATGATCCCTTTGGATAGCAATGAGTTATCTTATCTGGTG
GCATTGGAGCAAGACAAAACCAGTCACGACCTGCAACAAGCGCTACTGCGGGAAGGGTTAATGGGTCAATATCAGCAAAT
TGAACGTTACTGGCAAGACACGCTGAAGCCTCAGTTACAACAGGCCAAACACCCTGATGAGGTGGCGGCCAGTGTGGCGG
ATTTTGTTCACCAACTTGATGCTTTGGTATTAGCCATTGACCATAAAACTGAACAACGGCTGTTACAGGTTACCCTGATC
CCGCTGGTATTCAGCCTTCTGACACTCAGCCTATTGTTGGCAATGATCTATTATTTGCGCCGCCGGTTACTGCGCCCTTG
GCTGAAGTTAATCTCAATGGCGAATGCCATTGGCCGAGGTGATTTTAGCCAACGCTATACGCTGGACTATCAGCGTGACG
AAATGGGGATGCTGGGTACGGCATTGAATCGAATGTCGCAAGAATTGTCATTGATCTACGGCGATCTTGAGCAGCGAGTC
GTAAATAAAACCGCTGATTTACAACAGAAAAATCAGGTTTTGGCGTTCCTTTATCACAGTAGCCGCCAACTGCATACTCA
TCAGCCATTAAGTGAGCGTTTAGCCCCTGTCATTGAACAGCTACAAGCGTTAACCACGCTAGAAAATGTACAAATTTGTT
TGTATGAAAACCATCTGTATCGTGACCATGTCAATCACAATGCGGATGCAGAATATCTGATGCCTAAAAACCGCCCGGCG
TCATTAACGATCAGCGGCCCTGTTCGCCCCATCAGCCACCCTGAGTTATGGCAATCACTGAGTTGGAGTTTAAGCGATAA
ACTCGGTCAATATGGTTTATTACTGGCAAAACAACCCCTCGATAACCCCCTCAATTCTGACCAGCAGCAACTGGTCGATA
TGTTAGTTGAGCAACTGACCAGCACACTGGCGTTAGAGAGCCAAGCCAAACATCAGCAACAGTTATTGTTAATGGAGGAG
CGTTCCGCCATTGCGCGGGAACTGCATGACTCAATCGCTCAATCCCTTTCCTGCCTGAAGATACAGATCAGCTGTTTGCA
AATGCAAACTCCGGACTTACCGGAAAACAGCAACCTATTGATAAAACAAATGCGTGATGAACTCAATATCGCCTACCGTC
AATTACGTGAGTTACTGACCACATTCCGTCTGAAACTAAATGAAGCCACTCTGGAGGCCGCGTTACAGGCTCTGGTGAAT
GAGTTCAGCGAAAGAGCTGGTCTATCGATTACCTTCAAATATCAATTACCTCCAGATCAGGTACCAAACCATCAAGCCAT
TCATATGGTAAATATTGTCCGTGAGGCGCTGAATAATATCTATAAGCACGCCCGTGCCAGCGAAGCCACGGTTAACGTCC
ACTTAGAACAAGGCGATGTGGTGATGTCCATAACCGATAATGGTTGTGGGATAGGGCAAGCCAGTGAACAACCCAATCAT
TATGGTCTGATGATCATGCAAGATCGCGCCAACAGTTTACATGGGCACTGTAGTATCAATCAGAAAGAGGGAGGCGGTAC
TGAGGTTCGAGTGCAATTTACCCCCGAGAATAATGACCTTGACTGA

Upstream 100 bases:

>100_bases
GCCGTTTCCAGGCGGTTTCGCATGATGATGACGGCCGCGGGTATGAAACCCCATTAGCGCTAACACTTCGGCGCTAAAAA
CGGTAAGGTAACGGCGCTTA

Downstream 100 bases:

>100_bases
TAATCTATCTTGAAGCACGCCAAAACCACTGATTTCTCGACGACACGCGACTTCAATAGACACGTCACTTAAATACAATA
CTTCAATAAATACAATGATC

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MVKGISHAMKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSMIPLDSNELSYLV
ALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLI
PLVFSLLTLSLLLAMIYYLRRRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRV
VNKTADLQQKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNADAEYLMPKNRPA
SLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEE
RSAIARELHDSIAQSLSCLKIQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVN
EFSERAGLSITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNGCGIGQASEQPNH
YGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDLD

Sequences:

>Translated_601_residues
MVKGISHAMKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSMIPLDSNELSYLV
ALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLI
PLVFSLLTLSLLLAMIYYLRRRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRV
VNKTADLQQKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNADAEYLMPKNRPA
SLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEE
RSAIARELHDSIAQSLSCLKIQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVN
EFSERAGLSITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNGCGIGQASEQPNH
YGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDLD
>Mature_601_residues
MVKGISHAMKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRMQSYRLLSMIPLDSNELSYLV
ALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQQAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLI
PLVFSLLTLSLLLAMIYYLRRRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRV
VNKTADLQQKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLYRDHVNHNADAEYLMPKNRPA
SLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPLDNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEE
RSAIARELHDSIAQSLSCLKIQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVN
EFSERAGLSITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDVVMSITDNGCGIGQASEQPNH
YGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDLD

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=595, Percent_Identity=47.8991596638655, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1788812, Length=598, Percent_Identity=31.7725752508361, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082311, Length=197, Percent_Identity=29.4416243654822, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68083; Mature: 68083

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGISHAMKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHH
QSYRLLSMIPLDSNELSYLVALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQ
HHHHHHHEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLTLSLLLAMIYYLR
HCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRV
HHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNKTADLQQKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLY
HHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCHH
RDHVNHNADAEYLMPKNRPASLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPL
HHHCCCCCCCCEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHEEECCCC
DNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIARELHDSIAQSLSCLK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVN
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFSERAGLSITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDV
HHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCE
VMSITDNGCGIGQASEQPNHYGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDL
EEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCCC
D
C
>Mature Secondary Structure
MVKGISHAMKRYLTPLVNQIALLMILLGMLGLAGMVISSWMAQSIQGNAHAINQAGSLRM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHH
QSYRLLSMIPLDSNELSYLVALEQDKTSHDLQQALLREGLMGQYQQIERYWQDTLKPQLQ
HHHHHHHEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKHPDEVAASVADFVHQLDALVLAIDHKTEQRLLQVTLIPLVFSLLTLSLLLAMIYYLR
HCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLLRPWLKLISMANAIGRGDFSQRYTLDYQRDEMGMLGTALNRMSQELSLIYGDLEQRV
HHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNKTADLQQKNQVLAFLYHSSRQLHTHQPLSERLAPVIEQLQALTTLENVQICLYENHLY
HHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCHH
RDHVNHNADAEYLMPKNRPASLTISGPVRPISHPELWQSLSWSLSDKLGQYGLLLAKQPL
HHHCCCCCCCCEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHEEECCCC
DNPLNSDQQQLVDMLVEQLTSTLALESQAKHQQQLLLMEERSAIARELHDSIAQSLSCLK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQISCLQMQTPDLPENSNLLIKQMRDELNIAYRQLRELLTTFRLKLNEATLEAALQALVN
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFSERAGLSITFKYQLPPDQVPNHQAIHMVNIVREALNNIYKHARASEATVNVHLEQGDV
HHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCE
VMSITDNGCGIGQASEQPNHYGLMIMQDRANSLHGHCSINQKEGGGTEVRVQFTPENNDL
EEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCEEEEEECCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]