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The map label for this gene is prsA [H]

Identifier: 22126180

GI number: 22126180

Start: 2531064

End: 2532029

Strand: Direct

Name: prsA [H]

Synonym: y2295

Alternate gene names: 22126180

Gene position: 2531064-2532029 (Clockwise)

Preceding gene: 22126178

Following gene: 22126182

Centisome position: 55.01

GC content: 50.0

Gene sequence:

>966_bases
ATGCCTGAGGTTCTTCTCGTGCCTGATATGAAGCTTTTTGCTGGTAACGCCACCCCGGAACTAGCACAACGTATTGCCAA
CCGTTTGTACACCAGCCTTGGTGACGCCGCAGTAGGTCGTTTTAGCGACGGCGAAGTGAGCGTGCAAATCAACGAAAATG
TACGCGGTGGTGATATTTTCATCATCCAGTCCACCTGCGCACCCACGAACGATAACCTGATGGAACTGGTTGTCATGGTT
GATGCCCTGCGTCGCGCCTCCGCAGGACGTATTACAGCTGTAATTCCTTACTTCGGCTACGCTCGTCAGGATCGCCGTGT
GCGTTCTGCCCGTGTGCCTATCACTGCCAAAGTTGTTGCCGATTTTCTCTCAAGTGTTGGGGTTGACCGTGTATTGACAG
TGGATCTTCACGCTGAACAGATCCAAGGCTTCTTTGACGTTCCGGTAGATAACGTTTTTGGTAGCCCAATCCTGCTGGAA
GATATGTTGCAGCAGAATCTGGAAAACCCAATTGTTGTTTCACCAGATATTGGTGGCGTCGTCCGTGCCAGAGCCATTGC
CAAGCTACTGAACGATACCGATATGGCGATTATCGACAAACGCCGCCCACGCGCTAACGTTTCTCAGGTGATGCATATCA
TCGGGGACGTTGCTGGCCGTGACTGCGTGTTAGTTGACGATATGATCGATACCGGTGGTACCTTGTGTAAAGCAGCTGAA
GCCTTGAAAGAGCGTGGTGCCAAGCGTGTCTTTGCGTACGCAACTCACCCAATCTTCTCGGGAAATGCTGTTGATAACAT
TAGAAATTCTGTTATCGATGAAGTGATTGTTTGCGACACCATTCCGTTGTCCGCTGAAATCAAAGCATTGAAAAACGTGC
GTACTCTGACGCTATCCGGCATGTTGGCTGAAGCCATCCGCCGTATCAGCAATGAAGAGTCGATCTCTGCAATGTTTGAA
CATTAA

Upstream 100 bases:

>100_bases
GGTTATCACTGTGTTTTGGCTTTAAATACCCGTATGTATATTGCAATCAGTATGATTAATCGCCCCTGATTACTGATATG
ACAATATCTTCTCTGGACGC

Downstream 100 bases:

>100_bases
TCAGTATCGGGTTATGGATACACGGCTGCGACGCAATTAGGTTGCAGCTAAACACGGCGGTGCTTTTTGCTGGTATCCCT
GCGGTATCCTTAGCCAAATG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MPEVLLVPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMV
DALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLE
DMLQQNLENPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAE
ALKERGAKRVFAYATHPIFSGNAVDNIRNSVIDEVIVCDTIPLSAEIKALKNVRTLTLSGMLAEAIRRISNEESISAMFE
H

Sequences:

>Translated_321_residues
MPEVLLVPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMV
DALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLE
DMLQQNLENPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAE
ALKERGAKRVFAYATHPIFSGNAVDNIRNSVIDEVIVCDTIPLSAEIKALKNVRTLTLSGMLAEAIRRISNEESISAMFE
H
>Mature_320_residues
PEVLLVPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPTNDNLMELVVMVD
ALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLED
MLQQNLENPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEA
LKERGAKRVFAYATHPIFSGNAVDNIRNSVIDEVIVCDTIPLSAEIKALKNVRTLTLSGMLAEAIRRISNEESISAMFEH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=318, Percent_Identity=47.7987421383648, Blast_Score=301, Evalue=4e-82,
Organism=Homo sapiens, GI4506129, Length=318, Percent_Identity=47.7987421383648, Blast_Score=300, Evalue=8e-82,
Organism=Homo sapiens, GI84875539, Length=320, Percent_Identity=47.5, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI28557709, Length=318, Percent_Identity=47.1698113207547, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=35.5685131195335, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=36.4431486880466, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=32.4137931034483, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=96.8253968253968, Blast_Score=617, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI17554702, Length=321, Percent_Identity=45.7943925233645, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI71989924, Length=321, Percent_Identity=45.7943925233645, Blast_Score=294, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=45.7413249211356, Blast_Score=294, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=45.8333333333333, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=34.0236686390533, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=45.3968253968254, Blast_Score=268, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6321776, Length=314, Percent_Identity=47.1337579617834, Blast_Score=268, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6319403, Length=323, Percent_Identity=44.2724458204334, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=202, Percent_Identity=41.0891089108911, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=41.7391304347826, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355239, Length=318, Percent_Identity=46.5408805031447, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45551540, Length=340, Percent_Identity=43.5294117647059, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=33.8028169014084, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=33.8028169014084, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=33.8028169014084, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=33.8028169014084, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=32.0855614973262, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=32.0855614973262, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=32.0855614973262, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34913; Mature: 34781

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVLLVPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIF
CCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEECCCCCCCEEE
IIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVA
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
DFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQQNLENPIVVSPDIGGV
HHHHHCCCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHHCCCCEEECCCCCHH
VRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAE
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHH
ALKERGAKRVFAYATHPIFSGNAVDNIRNSVIDEVIVCDTIPLSAEIKALKNVRTLTLSG
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
MLAEAIRRISNEESISAMFEH
HHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
PEVLLVPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIF
CCEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEECCCCCCCEEE
IIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVA
EEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
DFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQQNLENPIVVSPDIGGV
HHHHHCCCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHHHCCCCEEECCCCCHH
VRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAE
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHHHHH
ALKERGAKRVFAYATHPIFSGNAVDNIRNSVIDEVIVCDTIPLSAEIKALKNVRTLTLSG
HHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
MLAEAIRRISNEESISAMFEH
HHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]