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The map label for this gene is ynfL [H]

Identifier: 22125998

GI number: 22125998

Start: 2325696

End: 2326619

Strand: Direct

Name: ynfL [H]

Synonym: y2109

Alternate gene names: 22125998

Gene position: 2325696-2326619 (Clockwise)

Preceding gene: 22125995

Following gene: 22125999

Centisome position: 50.55

GC content: 45.35

Gene sequence:

>924_bases
TTGTCTAATATATTAATCATTGCAAATAATACGTTTAAAATATCAATGAGTATTGAACTAAGACATTTGCGGTATTTTAT
TGCTGTCGCGGAAGAGCTGCACTTTGGCCGGGCAGCTGAACGCTTGCATATTTCTCAACCCCCCCTGAGCCAGCAAATCC
AAATCCTTGAAGAACAGATTGGGGCCCGCTTATTGGCACGAAATAAGCGTAATATCAGTCTCACCCAAGCGGGAAAGCTC
TTTTTAAAAGAGTCCTACCAAATTTTGGCACAGGTCAACTCTGCAACTGAGAAAGCAGCCTGTCTACATCGTGGGGAGTC
GGGAGTGCTGACGATCGGTTTCACTTCATCGGCACCATTGATCAATGTCGTTTCTAAGAATTTGCGTACTTTTCGGCAAA
TATATCCACAAGTACATATAAAAATGCAGGAGGTAAGTACCAAACAGCAAATTGAAGCATTACTCGACGGGCGGTTAGAT
CTAGGTATTATGCGTAATACTCGGCTGCCTGAGGCATTACAATATCGATTGCTATTACGTGAACCTCTGGTCGCTGTCGT
CCATGAAGATCATCCATTAGCCTCCCTACCAAATGGCAGTGTCAAATTTAGCTCACTGGACAAACAACCTTTTGTCTTCT
TTTCACGTGAAGTGGGGACTGCACTTTATGACGAATTCTTTACCTTATTAACCCGCGCGGGGATCACACCTTATATTACG
CAGGAGGTCGGCGAGGCGATGACCATCATAGGCTTGGTATCATCTGGTCTGGGGGTTTCAATCCTGCCCGCCTCGTTTAG
GCGAGTAAACGTTGATGGTGTCAAATACTTATCACTGGACGAAGCTGATGCGACGACAGAAATGTGGTTGGTTAATCATA
AGCATCGTCCGATGCCCCCCCTGCAGAAGCATTGCTCCGTTTGA

Upstream 100 bases:

>100_bases
ACGGTTAAATTAGTACGCAAGCAAGTTAACTCACGGAAAAATTAATACACAATAAATCATTATCGACAGAACATTTTAAT
CATAGAAATAGTTTGTTTTT

Downstream 100 bases:

>100_bases
TGATGGCTGATATTCAAGCTGCTCCGTATAAATAGTCACCATATTAAGGAAAAAAGAAAACACATGATTATGTGCAGCAA
ATCACATAACTAATCAAATA

Product: transcriptional regulator LYSR-type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSNILIIANNTFKISMSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLTQAGKL
FLKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLD
LGIMRNTRLPEALQYRLLLREPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYIT
QEVGEAMTIIGLVSSGLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPLQKHCSV

Sequences:

>Translated_307_residues
MSNILIIANNTFKISMSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLTQAGKL
FLKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLD
LGIMRNTRLPEALQYRLLLREPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYIT
QEVGEAMTIIGLVSSGLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPLQKHCSV
>Mature_306_residues
SNILIIANNTFKISMSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQIGARLLARNKRNISLTQAGKLF
LKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPLINVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLDL
GIMRNTRLPEALQYRLLLREPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYITQ
EVGEAMTIIGLVSSGLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPPLQKHCSV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=58.2191780821918, Blast_Score=347, Evalue=4e-97,
Organism=Escherichia coli, GI1788887, Length=259, Percent_Identity=38.2239382239382, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=34.0909090909091, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790399, Length=291, Percent_Identity=30.2405498281787, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI157672245, Length=263, Percent_Identity=32.6996197718631, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=30.1960784313725, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=26.984126984127, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87081904, Length=184, Percent_Identity=35.3260869565217, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=28.90625, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790208, Length=254, Percent_Identity=27.1653543307087, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790262, Length=247, Percent_Identity=27.1255060728745, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789506, Length=249, Percent_Identity=25.3012048192771, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=33.0769230769231, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=297, Percent_Identity=25.2525252525253, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787589, Length=145, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=27.0718232044199, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788296, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=285, Percent_Identity=26.3157894736842, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1787806, Length=252, Percent_Identity=23.015873015873, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34423; Mature: 34291

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNILIIANNTFKISMSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQI
CCCEEEEECCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHH
GARLLARNKRNISLTQAGKLFLKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPL
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
INVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQYRLLLR
HHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
EPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYIT
CCCEEEEECCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHH
QEVGEAMTIIGLVSSGLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPP
HHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCH
LQKHCSV
HHHHCCC
>Mature Secondary Structure 
SNILIIANNTFKISMSIELRHLRYFIAVAEELHFGRAAERLHISQPPLSQQIQILEEQI
CCEEEEECCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHH
GARLLARNKRNISLTQAGKLFLKESYQILAQVNSATEKAACLHRGESGVLTIGFTSSAPL
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
INVVSKNLRTFRQIYPQVHIKMQEVSTKQQIEALLDGRLDLGIMRNTRLPEALQYRLLLR
HHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
EPLVAVVHEDHPLASLPNGSVKFSSLDKQPFVFFSREVGTALYDEFFTLLTRAGITPYIT
CCCEEEEECCCCCCCCCCCCEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHH
QEVGEAMTIIGLVSSGLGVSILPASFRRVNVDGVKYLSLDEADATTEMWLVNHKHRPMPP
HHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCH
LQKHCSV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]