Click here to switch to the map view.

The map label for this gene is cls [H]

Identifier: 22125925

GI number: 22125925

Start: 2240946

End: 2242427

Strand: Reverse

Name: cls [H]

Synonym: y2033

Alternate gene names: 22125925

Gene position: 2242427-2240946 (Counterclockwise)

Preceding gene: 22125928

Following gene: 161484808

Centisome position: 48.74

GC content: 46.56

Gene sequence:

>1482_bases
GTGAACAGGATTTACGCCCTTATGACGACATTTTATACCGTAATCAGTTGGCTTTCCGTTTTTGGCTACTGGTTACTCAT
TGCCGGGGTAACATTGCGTATTCTGATGAAACGCAGGGCCGTTCCTTCTGCTATGGCTTGGTTACTGATTATCTACATTC
TGCCATTGGTGGGGATAATTGCTTACCTTTCATTCGGTGAATTGCATTTGGGTAAGCGGCGTGCTGAACGAGCTAAAGCC
ATGTGGCCATCGACCGCTCGCTGGCTGAGTGAACTCAAGGAATGCCAACATATCTTTGCTAACTCTAATAGCGAAGTTGC
CAGCCCACTGTTTCAATTATGTGAACGGCGTCAAGGAATAAACGGTGTTAAAGGAAATCAACTACAACTATTAACAACAA
CCGATGACACACTAAAAGCGCTAGTACGAGATATTGAACTCGCCCGCCACAATATAGAAATGGTGTTTTATATTTGGCAA
CCTGGTGGTCTGGTCGATCAGGTGGCTGAATCGTTGATGGCTGCGGCTCGACGCGGCGTTCATTGCCGGCTGTTACTCGA
TTCAGCGGGTAGCAAGCAATTTTTCCGCAGCCCTTATCCCGCAATGATGCGTAATGCAGGTATTGAGGTGGTTGAGGCAC
TTAAAGTTAATGTATTTCGTATGTTCTTGCGCCGAATGGATCTGCGCCAACATCGGAAAATTGTGTTAATCGACAACTAT
GTTGCTTATACCGGCAGTATGAACATGGTAGATCCCCGGTTCTTCAAACAAGATGCCGGGGTTGGTCAGTGGATTGATAT
GATGGCGCGCATGGAAGGCCCGGTAGCCACAACCCTAGGTATTGTTTATGCCTGTGACTGGGAAATTGAAACAGGCAAAC
GTATTTTACCCCCGCCCCCCGACGCGAATATTATGCCATTCGAAGAAGAGACTGGTCATACGATTCAAGTCATCGCTTCT
GGCCCTGGTTTCCCAGAAGAAATGATCCACCAGGCATTATTAACAGCTGTGTATGCCGCACGTGAACAGTTAATTATGAC
TACGCCATATTTTGTCCCCAGTGATGACTTATTACATGCCATCTGTACCGCGGCACAACGCGGGGTTGATGTCAGTATTA
TTGTCCCGCGTGAGAATGATTCGATGATGGTTCGCTGGGCCAGCCGAGCATTCTTCACCGAATTGCTGAATGCAGGCGTT
AAAATTTATCAGTTTGAAGGCGGCCTATTACACAGTAAAAGTGTTCTGGTCGATGGACAGCTAAGTTTAGTCGGCACTGT
TAATCTGGATATGCGCAGTTTGTGGCTGAATTTTGAAATCACACTGGTGATTGATGATGATGGCTTTGGGGCTGATTTAG
CACAAGTTCAGGATGATTATATCGCCCGCTCTGCCTTATTGGATGGTGAACGGTGGAACAAGCGCCCTCTTTGGCACCGA
GTAACTGAGCGTTTATTCTATTTTTTCAGCCCATTATTGTAA

Upstream 100 bases:

>100_bases
AGTTGCAAGTCCTTTTAGATGAAAAGATTTTATACATCAATATAACCGATTAAAGGTTTCACCTTCTCGATACTGTGCGT
ACACTCTTTAGTGACCGTTT

Downstream 100 bases:

>100_bases
AAAACCGAAGAATTCATGACCACGAAATTGCAGATTATCTGAATGAGCGGTATTGGGCAGGATAAAAATTGTAAAAAACC
TAGCGAGAATGTTTTAATTA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNRIYALMTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERAKA
MWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQ
PGGLVDQVAESLMAAARRGVHCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNY
VAYTGSMNMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPFEEETGHTIQVIAS
GPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGV
KIYQFEGGLLHSKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGERWNKRPLWHR
VTERLFYFFSPLL

Sequences:

>Translated_493_residues
MNRIYALMTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERAKA
MWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQ
PGGLVDQVAESLMAAARRGVHCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNY
VAYTGSMNMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPFEEETGHTIQVIAS
GPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGV
KIYQFEGGLLHSKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGERWNKRPLWHR
VTERLFYFFSPLL
>Mature_493_residues
MNRIYALMTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERAKA
MWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGINGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQ
PGGLVDQVAESLMAAARRGVHCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNY
VAYTGSMNMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPPDANIMPFEEETGHTIQVIAS
GPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHAICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGV
KIYQFEGGLLHSKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGERWNKRPLWHR
VTERLFYFFSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=83.5390946502058, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=392, Percent_Identity=27.0408163265306, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55990; Mature: 55990

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIYALMTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AYLSFGELHLGKRRAERAKAMWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGI
HHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
NGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQVAESLMAAARRGV
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCC
HCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNY
EEEEEEECCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECE
VAYTGSMNMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPP
EEEECCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCC
DANIMPFEEETGHTIQVIASGPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHA
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHH
ICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEGGLLHSKSVLVDGQ
HHHHHHCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCEEECCEEEECCC
LSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGERWNKRPLWHR
EEEEEEECCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VTERLFYFFSPLL
HHHHHHHHHHCCC
>Mature Secondary Structure
MNRIYALMTTFYTVISWLSVFGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AYLSFGELHLGKRRAERAKAMWPSTARWLSELKECQHIFANSNSEVASPLFQLCERRQGI
HHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
NGVKGNQLQLLTTTDDTLKALVRDIELARHNIEMVFYIWQPGGLVDQVAESLMAAARRGV
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHCCC
HCRLLLDSAGSKQFFRSPYPAMMRNAGIEVVEALKVNVFRMFLRRMDLRQHRKIVLIDNY
EEEEEEECCCCHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECE
VAYTGSMNMVDPRFFKQDAGVGQWIDMMARMEGPVATTLGIVYACDWEIETGKRILPPPP
EEEECCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCC
DANIMPFEEETGHTIQVIASGPGFPEEMIHQALLTAVYAAREQLIMTTPYFVPSDDLLHA
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHH
ICTAAQRGVDVSIIVPRENDSMMVRWASRAFFTELLNAGVKIYQFEGGLLHSKSVLVDGQ
HHHHHHCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCEEECCEEEECCC
LSLVGTVNLDMRSLWLNFEITLVIDDDGFGADLAQVQDDYIARSALLDGERWNKRPLWHR
EEEEEEECCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
VTERLFYFFSPLL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA