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The map label for this gene is eptA [H]

Identifier: 22125852

GI number: 22125852

Start: 2159708

End: 2161237

Strand: Reverse

Name: eptA [H]

Synonym: y1960

Alternate gene names: 22125852

Gene position: 2161237-2159708 (Counterclockwise)

Preceding gene: 22125854

Following gene: 22125851

Centisome position: 46.98

GC content: 43.07

Gene sequence:

>1530_bases
TTGATCCGATTCGACAATAGCGACAGCTATTTTTTTGCAGCAACCATTCCAGTCGTGATTTTCTGTGCACTGAATATTAT
TTTTAGCTTATTAACTCTCCCTCTGTTACGTAAGCCTATTATTATTTTATTCCTGTTGGGAAGTGCGGTGGCTAACTACT
TTATGTTCAGCTATGGTGCTGTTATCGATGCCAATATGATGCAGAATGCCTTTGAAACTAACTCACAGGAAGCCACCGCC
CTTTTCACTCCTCGTATGGCATTGTGGCTAATAACATTAGGTATTTTGCCTGCCATGATTGTCTGCTTTGTGCGAATTCG
AGCGACTCGCCCTTGGTGGTATATGCTGGGGTTACGGGTAGCAAATATTTTAATGTCAATGGTAATAATTTTTCTTATCG
CAGTCTTGTTCTATAAAGATTATGCGTCGCTGATCCGCAATAATAAAAATATCGTCAAAATGCTGACACCATCAAATTTT
GTTAGCGGCAGTTTTCAATTTGCCAAACACAAGTATTTTATCAGTAACATGCCATTGGTAAAAATAGGTGAAGACGCCCA
TAAGGGCCCAGTGATTAATGCACAACAAAATAAAACGCTGGTGATTTTGGTGGTGGGTGAAACCGCCCGGTCAGAAAACT
TTTCATTGGGCGGATATGCTCGGGAAACGAACCCTCGCCTGCAACAACAAGATATCATTTATTTCAAACATGCCTCATCC
TGTGGCACTGAAACGGCGGTCTCTGTCCCCTGTATGTTCTCTAATATGCCACGCAAAAGTTATGACGCCTCCCTGGCGAG
TCATCAGGAAGGGTTAATGGATATTATTGCGCGCGCTGGATTGAGTGTATTGTGGCGTGAAAATGATGGTGGCTGTAAAG
GCGCTTGTGACCGTATTCCACACCAGGATGTGACGCAATTGCAACTTAACGCAGATTGTCAGGATGGGGTATGTTTAGAT
AATGCGCTTTTATATAAACTGGATAACTATATTAATGGATTACAGAATGACGGTGTAATCGTCTTACACCAAATGGGCAG
CCACGGGCCCGCATACTATCGCCGTACCAGCCCGGAGTTGACTAAATTCACCCCGACGTGTAATAGCAACCAGATTCAGG
ACTGCACCGCCCAAGAACTGGTCAATACATACGATAACTCTATTCTCTATACTGATGCCATGCTGGATGACACCATTGCC
TTGTTGAAACAGCACAGTGACCGATTTAATACGGCGTTGGTCTATCTTTCAGACCATGGTGAATCACTCGGTGAAAACGG
CCTCTATTTACATGGTACACCTTATGTATTTGCCCCAAGCCAGCAAACTCATATCCCTTTTCTGATGTGGTTATCACCAG
AATATACAAAAAGCTATGGTATTGACCGTCAATGCTTATCACATAGCGCACAACAGGATGAAGTGTCTCAGGATAATCTC
TTCCATACCCTATTAGGGATGGTAAACATTCAGACCAACGAATACTTATCAGGCATGGATTTACTGCAAAAATGCCGGAG
TCTGCAATAG

Upstream 100 bases:

>100_bases
ATAATATAAATTACAGTGTAACGGATTAACATTTATTCTGGCCTGTGCACTGTTTTTTACCTTCTTCCAAAATGCACTGT
TTATTTATAAAGCCTGGTCG

Downstream 100 bases:

>100_bases
CGATGGCTGTATCAACGAGAAGCCCATCGTGGCCTTGGACAGGATATTTTCCTCAATCAAAAAATTAATCAGATAAAACA
GTCAATTAACAAGATAAAAC

Product: putative cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATA
LFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANILMSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNF
VSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILVVGETARSENFSLGGYARETNPRLQQQDIIYFKHASS
CGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLD
NALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFTPTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIA
LLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTHIPFLMWLSPEYTKSYGIDRQCLSHSAQQDEVSQDNL
FHTLLGMVNIQTNEYLSGMDLLQKCRSLQ

Sequences:

>Translated_509_residues
MIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATA
LFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANILMSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNF
VSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILVVGETARSENFSLGGYARETNPRLQQQDIIYFKHASS
CGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLD
NALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFTPTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIA
LLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTHIPFLMWLSPEYTKSYGIDRQCLSHSAQQDEVSQDNL
FHTLLGMVNIQTNEYLSGMDLLQKCRSLQ
>Mature_509_residues
MIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGAVIDANMMQNAFETNSQEATA
LFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRVANILMSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNF
VSGSFQFAKHKYFISNMPLVKIGEDAHKGPVINAQQNKTLVILVVGETARSENFSLGGYARETNPRLQQQDIIYFKHASS
CGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIPHQDVTQLQLNADCQDGVCLD
NALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPELTKFTPTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIA
LLKQHSDRFNTALVYLSDHGESLGENGLYLHGTPYVFAPSQQTHIPFLMWLSPEYTKSYGIDRQCLSHSAQQDEVSQDNL
FHTLLGMVNIQTNEYLSGMDLLQKCRSLQ

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=494, Percent_Identity=53.6437246963563, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI87082286, Length=440, Percent_Identity=25.2272727272727, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790392, Length=485, Percent_Identity=24.3298969072165, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 57196; Mature: 57196

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGA
CEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
VIDANMMQNAFETNSQEATALFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRV
HEEHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ANILMSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHEEEECCCCEE
KIGEDAHKGPVINAQQNKTLVILVVGETARSENFSLGGYARETNPRLQQQDIIYFKHASS
EECCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
CGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIP
CCCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCC
HQDVTQLQLNADCQDGVCLDNALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPEL
CCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEECCCCCC
TKFTPTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHG
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCC
ESLGENGLYLHGTPYVFAPSQQTHIPFLMWLSPEYTKSYGIDRQCLSHSAQQDEVSQDNL
CCCCCCCEEEECCCEEECCCCCCCCCEEEEECCHHHHHCCCCHHHHHCCCCCCCCCHHHH
FHTLLGMVNIQTNEYLSGMDLLQKCRSLQ
HHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRFDNSDSYFFAATIPVVIFCALNIIFSLLTLPLLRKPIIILFLLGSAVANYFMFSYGA
CEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
VIDANMMQNAFETNSQEATALFTPRMALWLITLGILPAMIVCFVRIRATRPWWYMLGLRV
HEEHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ANILMSMVIIFLIAVLFYKDYASLIRNNKNIVKMLTPSNFVSGSFQFAKHKYFISNMPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHEEEECCCCEE
KIGEDAHKGPVINAQQNKTLVILVVGETARSENFSLGGYARETNPRLQQQDIIYFKHASS
EECCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCC
CGTETAVSVPCMFSNMPRKSYDASLASHQEGLMDIIARAGLSVLWRENDGGCKGACDRIP
CCCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCC
HQDVTQLQLNADCQDGVCLDNALLYKLDNYINGLQNDGVIVLHQMGSHGPAYYRRTSPEL
CCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEECCCCCC
TKFTPTCNSNQIQDCTAQELVNTYDNSILYTDAMLDDTIALLKQHSDRFNTALVYLSDHG
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCC
ESLGENGLYLHGTPYVFAPSQQTHIPFLMWLSPEYTKSYGIDRQCLSHSAQQDEVSQDNL
CCCCCCCEEEECCCEEECCCCCCCCCEEEEECCHHHHHCCCCHHHHHCCCCCCCCCHHHH
FHTLLGMVNIQTNEYLSGMDLLQKCRSLQ
HHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]