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The map label for this gene is hisJ [H]

Identifier: 22125502

GI number: 22125502

Start: 1776638

End: 1777693

Strand: Direct

Name: hisJ [H]

Synonym: y1607

Alternate gene names: 22125502

Gene position: 1776638-1777693 (Clockwise)

Preceding gene: 22125501

Following gene: 22125503

Centisome position: 38.62

GC content: 44.41

Gene sequence:

>1056_bases
TTGCGTGCAGGAAGGTGGCAACCAAGCGAATCTTCAGGGCCTTATAGAAGGCAAGTGACTGCGGGTGAACGAAGGCGGTC
AGCACAGGCAATTTGCAGCATGACGGGTATATCGATTTTAGTCGTTATCGTGCAAAGAAACAGTAGGGTGGAATTGATTT
TCCCTCCAGATAATTACTGGCACGATTACTGCAAATTGATTTATGGACGCTCAGGCTTGGACAGAAATCTAACGCGCTTT
GTGGCATCAAAAATCAATTTGAGGGTTATGTTTATGAATAAAAAGCTGCGAGTTCTTCCTTTGGTATTCGCATTCGCTGC
GGCTAGTAGTGCTTTTGCCGCCATTCCAAAAGAGATCAAAATTGGCACAGATCCGACTTACGCCCCTTTTGAATCGAAAA
ACTCGAGTGGTGAATTAGTGGGTTTTGATATCGATATCGCGAAGGAGTTATGCAAACGTATAGATACCAAATGTACTTTT
GTTGAAAGTGACTTTGATGCGCTAATCCCGTCGCTGAAAGCGAAAAAAGTCGATGCCATTATCTCCTCTCTCTCCATTAC
TGAAAAACGCCAAAAAGAGATTGCCTTCACCGATAAACTCTTTGCCGCCAATTCCCGTTTAATTGCTGCTAAAGGTAGCA
CTATTGAGCCGACACTGGTGGCATTAAAAGGTAAGCGCGTGGGGGTGTTACAAGGGTCGACTCAGGAAGCTTTTGCCAAT
GCTGAGTGGCAGCCAAAAGGCATTGATGTTGTTGCTTATCAAAATCAGGATCTGATTTATGCAGATCTGGCCTCTGGCCG
TATTGATGCGGCTTTCCAGGATGAAGTGGCCGCCAGTGAAGGTTTCCTAAAACAACCTGTGGGTAAAGATTATATCTTTG
CCGGTCCATCAGTAAAAAATGATCAATTCTTCGGTGTAGGGACTGGCATGGGGTTGCGCAAAGCCGATGTTGAGCTGAAA
GAGGCGTTAAATAAAGCGTTTGCTGAAATGCGTAATGACGGCACCTACGATCAATTTGCCAAAAAATACTTCGATTTTGA
TGTTTACGGTGATTAA

Upstream 100 bases:

>100_bases
GCTCCACAATGATCCTGCAACAGCGATGGTGCTGTAGTAGGGTTTTTGTATTCTGTTTCTGTTCACGACCAGATGTCATG
ACATTTATACCCTAAATAAT

Downstream 100 bases:

>100_bases
TCCTTTTTGTCGTCGTGGCCTCTGTCGATTGTGGCAGGGGCTAACATACCGTAATTAACCAGACTCCTCACTCAGTAAAA
CAGGATAACCCACAGATCAC

Product: histidine-binding periplasmic protein of high-affinity histidine transport system

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRAGRWQPSESSGPYRRQVTAGERRRSAQAICSMTGISILVVIVQRNSRVELIFPPDNYWHDYCKLIYGRSGLDRNLTRF
VASKINLRVMFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTF
VESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFAN
AEWQPKGIDVVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELK
EALNKAFAEMRNDGTYDQFAKKYFDFDVYGD

Sequences:

>Translated_351_residues
MRAGRWQPSESSGPYRRQVTAGERRRSAQAICSMTGISILVVIVQRNSRVELIFPPDNYWHDYCKLIYGRSGLDRNLTRF
VASKINLRVMFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTF
VESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFAN
AEWQPKGIDVVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELK
EALNKAFAEMRNDGTYDQFAKKYFDFDVYGD
>Mature_351_residues
MRAGRWQPSESSGPYRRQVTAGERRRSAQAICSMTGISILVVIVQRNSRVELIFPPDNYWHDYCKLIYGRSGLDRNLTRF
VASKINLRVMFMNKKLRVLPLVFAFAAASSAFAAIPKEIKIGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTF
VESDFDALIPSLKAKKVDAIISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFAN
AEWQPKGIDVVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKNDQFFGVGTGMGLRKADVELK
EALNKAFAEMRNDGTYDQFAKKYFDFDVYGD

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=78.8135593220339, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=68.8461538461538, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=39.1836734693878, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=33.7301587301587, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=32.1739130434783, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=28.4, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 38886; Mature: 38886

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGRWQPSESSGPYRRQVTAGERRRSAQAICSMTGISILVVIVQRNSRVELIFPPDNYW
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCHHH
HDYCKLIYGRSGLDRNLTRFVASKINLRVMFMNKKLRVLPLVFAFAAASSAFAAIPKEIK
HHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEE
IGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALIPSLKAKKVDAI
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHH
ISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFAN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCEEEEEECCCHHHHCC
AEWQPKGIDVVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKN
CCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHCCCHHHCCCCCCEEEECCCCCC
DQFFGVGTGMGLRKADVELKEALNKAFAEMRNDGTYDQFAKKYFDFDVYGD
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEECCC
>Mature Secondary Structure
MRAGRWQPSESSGPYRRQVTAGERRRSAQAICSMTGISILVVIVQRNSRVELIFPPDNYW
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEECCCHHH
HDYCKLIYGRSGLDRNLTRFVASKINLRVMFMNKKLRVLPLVFAFAAASSAFAAIPKEIK
HHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEE
IGTDPTYAPFESKNSSGELVGFDIDIAKELCKRIDTKCTFVESDFDALIPSLKAKKVDAI
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEECCHHHHHCCHHHHHHHHH
ISSLSITEKRQKEIAFTDKLFAANSRLIAAKGSTIEPTLVALKGKRVGVLQGSTQEAFAN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCEEEEEECCCHHHHCC
AEWQPKGIDVVAYQNQDLIYADLASGRIDAAFQDEVAASEGFLKQPVGKDYIFAGPSVKN
CCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHCCCHHHCCCCCCEEEECCCCCC
DQFFGVGTGMGLRKADVELKEALNKAFAEMRNDGTYDQFAKKYFDFDVYGD
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]