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The map label for this gene is folC [H]

Identifier: 22125497

GI number: 22125497

Start: 1771007

End: 1772389

Strand: Direct

Name: folC [H]

Synonym: y1602

Alternate gene names: 22125497

Gene position: 1771007-1772389 (Clockwise)

Preceding gene: 161484834

Following gene: 161484833

Centisome position: 38.49

GC content: 53.22

Gene sequence:

>1383_bases
TTGGGAGGAACAACGGGAGCTAGCGTACAGGCAACTTGGAGTATCGCGGGGATAAAACCAAGGGAAGTGACGGGACTCAT
GAACAATCATCAAACTCCCCAAGCCACGTCGCCTTTGGCCGCGTGGCTTTGCTATCTTGAGCATTTGCATTCTCAGCCCA
TTGAGTTGGGTCTGGAACGTGTTAAACAGGTTGCTGAGCGTCTGGATCTGCTTAAACCTGCCCCCAAAATTTTTACCGTT
GCCGGTACCAACGGCAAAGGTACCACTTGTTGTACATTGGAAGCCATTTTATTGGCGGCCGGTCTGCGAGTCGGGGTCTA
CAGCTCCCCCCACCTTTTACGCTATACCGAGCGGGTTCGAATTCAGGGGCAAGAGCTATCTGAGGCAGAACACAGCCACT
CTTTTGCCCAGATCGAAGCCGGGCGAGGTGATATCTCACTGACCTACTTTGAATTTGGCACCTTATCAGCGTTGCAACTA
TTTAAGCAGGCTAAACTTGATGTGGTGATCCTGGAAGTCGGGTTGGGTGGGCGGTTAGATGCGACCAATATTGTTGACTC
TGATGTCGCCGCCATCACCAGTATCGCGTTAGATCATACTGATTGGCTGGGTTATGACCGTGAAAGCATTGGCCGCGAGA
AGGCGGGTGTCTTCCGTGGCGGTAAACCTGCGGTCGTGGGTGAACCAGATATGCCCCAGTCTATTGCCGATGTGGCGGCT
GAGTTAGGCGCACAGCTCTATCGTCGGGATGTTGCCTGGAAATTCAGCCAGCAAGAACCGTTTGATCAACAAGAACCCGT
TGATCAACAAATAAACGGCTGGCACTGGCAGTGCGGTGAGCGCCAATTAACCGGCTTACCGGTGCCGAACGTGCCGCTGG
CGAATGCGGCTACGGCGCTGGCTGTACTGCATTATTCTGAGTTGCCATTGAGTGACGAGGCGATTCGGCAGGGGTTACAG
GCCGCCAGTTTACCAGGGCGTTTTCAGGTCGTCAGTGAGCAACCGTTGCTGATTCTGGATGTCGCCCATAATCCGCATGC
GGCCCGTTATCTGGTCAATCGGTTGGCCCAAGTTATTAACCCGGTTAATGCGTCGAAACAAGGCAAAGTGCGGGCAGTCG
TCGGGATGCTATCGGATAAAGATATTGCGGGTACGCTGGCATGTTTATCTGAGCGAGTTGATGAGTGGTATTGCGCTCCG
CTTGAAGGGCCACGAGGAGCCAGTGCTGGGCAATTGGCCGAACATCTGGTGAGCGCACGTCAGTTTAGTGATGTAGAAAC
TGCCTGGCGCCAGGCTATGCAAGATGCAGATACACAGGATGTGGTCATTGTCTGTGGCTCTTTCCATACTGTTGCCCATG
TTATGGCAGCATTGCATTTGTGA

Upstream 100 bases:

>100_bases
TTAATAACGACAATTTGGCAATAACGAGTGTGTAATATGCCCTAAATAATTCGAGTTGCAGGAGAGCGGTAACCGAGTGA
GTCCCTAAGCATTTTCGATA

Downstream 100 bases:

>100_bases
CAGTGTCCAGTTTATAACAGATTGAATTTAAACCCAAAGAGCATGGAGTTGCAGGCGGGCAGTAAACGATCGCTTCCTGC
ATCTTCAAGTCCGGCGGGAG

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MGGTTGASVQATWSIAGIKPREVTGLMNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTV
AGTNGKGTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQL
FKQAKLDVVILEVGLGGRLDATNIVDSDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAA
ELGAQLYRRDVAWKFSQQEPFDQQEPVDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQ
AASLPGRFQVVSEQPLLILDVAHNPHAARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAP
LEGPRGASAGQLAEHLVSARQFSDVETAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL

Sequences:

>Translated_460_residues
MGGTTGASVQATWSIAGIKPREVTGLMNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTV
AGTNGKGTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQL
FKQAKLDVVILEVGLGGRLDATNIVDSDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAA
ELGAQLYRRDVAWKFSQQEPFDQQEPVDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQ
AASLPGRFQVVSEQPLLILDVAHNPHAARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAP
LEGPRGASAGQLAEHLVSARQFSDVETAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL
>Mature_459_residues
GGTTGASVQATWSIAGIKPREVTGLMNNHQTPQATSPLAAWLCYLEHLHSQPIELGLERVKQVAERLDLLKPAPKIFTVA
GTNGKGTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLF
KQAKLDVVILEVGLGGRLDATNIVDSDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAAE
LGAQLYRRDVAWKFSQQEPFDQQEPVDQQINGWHWQCGERQLTGLPVPNVPLANAATALAVLHYSELPLSDEAIRQGLQA
ASLPGRFQVVSEQPLLILDVAHNPHAARYLVNRLAQVINPVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAPL
EGPRGASAGQLAEHLVSARQFSDVETAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=375, Percent_Identity=30.9333333333333, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI66932984, Length=375, Percent_Identity=30.9333333333333, Blast_Score=132, Evalue=6e-31,
Organism=Escherichia coli, GI1788654, Length=428, Percent_Identity=65.4205607476635, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17553150, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=310, Percent_Identity=31.9354838709677, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=331, Percent_Identity=29.607250755287, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324815, Length=276, Percent_Identity=29.7101449275362, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641571, Length=177, Percent_Identity=38.4180790960452, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24581568, Length=203, Percent_Identity=34.4827586206897, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 49800; Mature: 49669

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGTTGASVQATWSIAGIKPREVTGLMNNHQTPQATSPLAAWLCYLEHLHSQPIELGLER
CCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
VKQVAERLDLLKPAPKIFTVAGTNGKGTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVR
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
IQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLD
CCCHHHHHHHHCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCC
ATNIVDSDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAA
CCCCCHHHHHHHHHHHHCCHHHCCCCHHHHCCHHHCCCCCCCCCEECCCCCCHHHHHHHH
ELGAQLYRRDVAWKFSQQEPFDQQEPVDQQINGWHWQCGERQLTGLPVPNVPLANAATAL
HHHHHHHHHHHHEEECCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHH
AVLHYSELPLSDEAIRQGLQAASLPGRFQVVSEQPLLILDVAHNPHAARYLVNRLAQVIN
HHHHHCCCCCCHHHHHCCHHHCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHC
PVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAPLEGPRGASAGQLAEHLVSAR
CCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHH
QFSDVETAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
GGTTGASVQATWSIAGIKPREVTGLMNNHQTPQATSPLAAWLCYLEHLHSQPIELGLER
CCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
VKQVAERLDLLKPAPKIFTVAGTNGKGTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVR
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
IQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLD
CCCHHHHHHHHCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCCC
ATNIVDSDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGKPAVVGEPDMPQSIADVAA
CCCCCHHHHHHHHHHHHCCHHHCCCCHHHHCCHHHCCCCCCCCCEECCCCCCHHHHHHHH
ELGAQLYRRDVAWKFSQQEPFDQQEPVDQQINGWHWQCGERQLTGLPVPNVPLANAATAL
HHHHHHHHHHHHEEECCCCCCCCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHHH
AVLHYSELPLSDEAIRQGLQAASLPGRFQVVSEQPLLILDVAHNPHAARYLVNRLAQVIN
HHHHHCCCCCCHHHHHCCHHHCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHC
PVNASKQGKVRAVVGMLSDKDIAGTLACLSERVDEWYCAPLEGPRGASAGQLAEHLVSAR
CCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHH
QFSDVETAWRQAMQDADTQDVVIVCGSFHTVAHVMAALHL
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]