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The map label for this gene is nanA [H]

Identifier: 22125355

GI number: 22125355

Start: 1616586

End: 1617485

Strand: Reverse

Name: nanA [H]

Synonym: y1458

Alternate gene names: 22125355

Gene position: 1617485-1616586 (Counterclockwise)

Preceding gene: 22125356

Following gene: 22125352

Centisome position: 35.16

GC content: 48.56

Gene sequence:

>900_bases
ATGCGTATGAAAAAGTTAACCGGCCTGATCGCAGCTCCGCACACTCCCTTTGATGAACAGGGCGAGGTCAATTATCCGGT
CATTGACCAAATTGCCGAGCATCTGATCAATGATGGTGTGAAAGGCGTTTATGTCTGTGGTACCACTGGCGAAGGCATTC
ATTGCTCAGTTGATGAGCGCAAGAAAATTGCTGAACGCTGGGTAAATGCCGCTCAAGGCAAACTGAGCATTACCTTACAC
ACCGGTGCTCTCAGTATTAAAGATGCGGTTGATCTCTCTCCTCATGCGGAAACACTGGATATCTTCGCAACCTCAGCCAT
CGGCCCCTGTTTCTTTAAACCGGGTAATCTGGATGACCTAATCGCTTATTGTCAGGCAATAGCCGCGGCAGCACCGTCGA
AGGGCTTCTATTACTACCACTCCGGTATGTCCGGTGTAAATTTGGACATGGAACAGTTTCTGATCAAAGCGGAATCCAAG
ATCCCTAACTTGTCAGGAATTAAATTCAATAACGCTGATTTGTATGAGTTTCAACGCTGTTTACGTGTGAGTGGCGGTAA
ATTTGATATTCCGTTTGGCGTTGATGAACATCTGCCAGGTGGATTAGCTGTAGGGGCTATTGGCGCTGTTGGCAGCACTT
ATAACTATGCCGCTCCGCTGTTCCATAAAATTATCGCTGACTTTAATGCGGGTGATCAGGTTGCGGTACAGCGTGGAATG
GATCACGTTATCGCCCTGATCCGTGTGCTGGTCGAATTCGGTGGTGTTGCCGCCGGTAAGGCCGCGATGCAATTACATGG
TATTGATGCCGGTAACCCACGTCTGCCACTGCGTGCCTTGACCAAAGAACAGAAGCAAACCGTGGTTAACAGAATGCGTG
ACGCTATTACGTTGCAGTAA

Upstream 100 bases:

>100_bases
ATGCAATTAAGCAGTGTGAAACAGAAAAACTGACCGAATACTGATTATCGTCATTATTGATTATGGCCCACAGGGCCCTC
CACTCTCCCAGTTTATAAGG

Downstream 100 bases:

>100_bases
TTATTTCTCTCTCTTCCCTAAGTGATAAATAATGAAAGGCCGGATTTCATCAATCCGGCAACGGTGATATATTTTGCTCG
ATACCCCATCCCCGATTTAC

Product: acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRMKKLTGLIAAPHTPFDEQGEVNYPVIDQIAEHLINDGVKGVYVCGTTGEGIHCSVDERKKIAERWVNAAQGKLSITLH
TGALSIKDAVDLSPHAETLDIFATSAIGPCFFKPGNLDDLIAYCQAIAAAAPSKGFYYYHSGMSGVNLDMEQFLIKAESK
IPNLSGIKFNNADLYEFQRCLRVSGGKFDIPFGVDEHLPGGLAVGAIGAVGSTYNYAAPLFHKIIADFNAGDQVAVQRGM
DHVIALIRVLVEFGGVAAGKAAMQLHGIDAGNPRLPLRALTKEQKQTVVNRMRDAITLQ

Sequences:

>Translated_299_residues
MRMKKLTGLIAAPHTPFDEQGEVNYPVIDQIAEHLINDGVKGVYVCGTTGEGIHCSVDERKKIAERWVNAAQGKLSITLH
TGALSIKDAVDLSPHAETLDIFATSAIGPCFFKPGNLDDLIAYCQAIAAAAPSKGFYYYHSGMSGVNLDMEQFLIKAESK
IPNLSGIKFNNADLYEFQRCLRVSGGKFDIPFGVDEHLPGGLAVGAIGAVGSTYNYAAPLFHKIIADFNAGDQVAVQRGM
DHVIALIRVLVEFGGVAAGKAAMQLHGIDAGNPRLPLRALTKEQKQTVVNRMRDAITLQ
>Mature_299_residues
MRMKKLTGLIAAPHTPFDEQGEVNYPVIDQIAEHLINDGVKGVYVCGTTGEGIHCSVDERKKIAERWVNAAQGKLSITLH
TGALSIKDAVDLSPHAETLDIFATSAIGPCFFKPGNLDDLIAYCQAIAAAAPSKGFYYYHSGMSGVNLDMEQFLIKAESK
IPNLSGIKFNNADLYEFQRCLRVSGGKFDIPFGVDEHLPGGLAVGAIGAVGSTYNYAAPLFHKIIADFNAGDQVAVQRGM
DHVIALIRVLVEFGGVAAGKAAMQLHGIDAGNPRLPLRALTKEQKQTVVNRMRDAITLQ

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=292, Percent_Identity=40.7534246575342, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1789620, Length=250, Percent_Identity=28.8, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082415, Length=236, Percent_Identity=28.3898305084746, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786463, Length=230, Percent_Identity=24.3478260869565, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788823, Length=292, Percent_Identity=22.2602739726027, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 32179; Mature: 32179

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00665 DHDPS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKKLTGLIAAPHTPFDEQGEVNYPVIDQIAEHLINDGVKGVYVCGTTGEGIHCSVDER
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEECHHHH
KKIAERWVNAAQGKLSITLHTGALSIKDAVDLSPHAETLDIFATSAIGPCFFKPGNLDDL
HHHHHHHHHHCCCEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHCCCCCEECCCCHHHH
IAYCQAIAAAAPSKGFYYYHSGMSGVNLDMEQFLIKAESKIPNLSGIKFNNADLYEFQRC
HHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHH
LRVSGGKFDIPFGVDEHLPGGLAVGAIGAVGSTYNYAAPLFHKIIADFNAGDQVAVQRGM
HHHCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCH
DHVIALIRVLVEFGGVAAGKAAMQLHGIDAGNPRLPLRALTKEQKQTVVNRMRDAITLQ
HHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRMKKLTGLIAAPHTPFDEQGEVNYPVIDQIAEHLINDGVKGVYVCGTTGEGIHCSVDER
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEECHHHH
KKIAERWVNAAQGKLSITLHTGALSIKDAVDLSPHAETLDIFATSAIGPCFFKPGNLDDL
HHHHHHHHHHCCCEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHCCCCCEECCCCHHHH
IAYCQAIAAAAPSKGFYYYHSGMSGVNLDMEQFLIKAESKIPNLSGIKFNNADLYEFQRC
HHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHH
LRVSGGKFDIPFGVDEHLPGGLAVGAIGAVGSTYNYAAPLFHKIIADFNAGDQVAVQRGM
HHHCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCH
DHVIALIRVLVEFGGVAAGKAAMQLHGIDAGNPRLPLRALTKEQKQTVVNRMRDAITLQ
HHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]