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The map label for this gene is rbsB [H]

Identifier: 22125052

GI number: 22125052

Start: 1297819

End: 1298748

Strand: Direct

Name: rbsB [H]

Synonym: y1149

Alternate gene names: 22125052

Gene position: 1297819-1298748 (Clockwise)

Preceding gene: 22125051

Following gene: 22125053

Centisome position: 28.21

GC content: 53.12

Gene sequence:

>930_bases
ATGAAAAAGCTGATCCTCGCCGCCCTCATCGTATTCACTTCTGGGGTGGCCTTTGCCGAGAATGAACAAATTGTTTTTAG
TACACCAAATCTGGCGATGCCGTTTGAAGTGCATATGCAGCGCACTGCCGTGAAAACGGCCAAAGCGTTGGGTGTGAAGT
TACAAGTACTGGATGGGCAGGGCAGTTCGCCTAAGCAGGTGGCTGACCTGGAAAATGCCATCACCCGTGGCGCGCAGGGT
TTTATTGTCTCGCCGAATGACATTAACGCCATTTCCAGTGCCGTAGAGGAAATTCAGCAGGCCAAACTGCCGGTGGTCAC
GCTGGATCGGTCAGTCGAGAGCGAGAAAAAAGTGCCGCATTTTGGCGCGAATAATTACAAAGGTGGCGAGGCCATTGCCA
ACTATGTGAAATCGATGTTCCCGAATGGCGCAGAGATCGTGCTACTCACCGGTCAGCTCGGTTCCTCCTCCAATATTGAA
CGAACCAAAGGGATCCGCGACAGCCTGAAAACGGGGGGTGACAAGTATCGTATCGTCGTCGATCAGACAGGAAACTGGAT
GCGGTCAGAAGGGATGCGCATTATAGAAAGCGTACTCCCATCGCTGCCAAAACCCCCGCAAGTGATCCTCTCAGCCAATG
ATGATATGGCGCTGGGTGCCATTGAAGCATTACAGAGCCATGGCATGAAACCGGGTGAAATTATGGTGACAGGTTTCGAT
GCCGTGCCAGAGGCGCTGGCACGTGTACGTGAAGGGTGGATGGCGGTGACGGCCGATCAACGTCCGGGTTACGCGGTGAC
AACCGCCATGAACCAACTGGTGGCGAATATCCGTGACCAAAAAGCGATCACCGGTGCAGATTTCTTGCCAACGATGATCA
CCAAAGAAAATCTAGACCAGGCCGAGCGCGTTAGCGAAGCGGGTAAGTAA

Upstream 100 bases:

>100_bases
CCGACCTTACAGCATCTATCGATCTTACAACATCTATCGATTCTACAACCCTCTAGACCTTACAACCGTACCGACTCTAC
AACGATAAAGAGAGATAACA

Downstream 100 bases:

>100_bases
CCCTGTCAGCGTCGCCGCGACGGGGGCGGCGTCAGGAGGAGAACACCGATGTCGCAACCTTTATTGAAAATCACCGATAT
GGCGAAAAGCTTCTCTGGTG

Product: periplasmic solute-binding protein of ABC transporter

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKLILAALIVFTSGVAFAENEQIVFSTPNLAMPFEVHMQRTAVKTAKALGVKLQVLDGQGSSPKQVADLENAITRGAQG
FIVSPNDINAISSAVEEIQQAKLPVVTLDRSVESEKKVPHFGANNYKGGEAIANYVKSMFPNGAEIVLLTGQLGSSSNIE
RTKGIRDSLKTGGDKYRIVVDQTGNWMRSEGMRIIESVLPSLPKPPQVILSANDDMALGAIEALQSHGMKPGEIMVTGFD
AVPEALARVREGWMAVTADQRPGYAVTTAMNQLVANIRDQKAITGADFLPTMITKENLDQAERVSEAGK

Sequences:

>Translated_309_residues
MKKLILAALIVFTSGVAFAENEQIVFSTPNLAMPFEVHMQRTAVKTAKALGVKLQVLDGQGSSPKQVADLENAITRGAQG
FIVSPNDINAISSAVEEIQQAKLPVVTLDRSVESEKKVPHFGANNYKGGEAIANYVKSMFPNGAEIVLLTGQLGSSSNIE
RTKGIRDSLKTGGDKYRIVVDQTGNWMRSEGMRIIESVLPSLPKPPQVILSANDDMALGAIEALQSHGMKPGEIMVTGFD
AVPEALARVREGWMAVTADQRPGYAVTTAMNQLVANIRDQKAITGADFLPTMITKENLDQAERVSEAGK
>Mature_309_residues
MKKLILAALIVFTSGVAFAENEQIVFSTPNLAMPFEVHMQRTAVKTAKALGVKLQVLDGQGSSPKQVADLENAITRGAQG
FIVSPNDINAISSAVEEIQQAKLPVVTLDRSVESEKKVPHFGANNYKGGEAIANYVKSMFPNGAEIVLLTGQLGSSSNIE
RTKGIRDSLKTGGDKYRIVVDQTGNWMRSEGMRIIESVLPSLPKPPQVILSANDDMALGAIEALQSHGMKPGEIMVTGFD
AVPEALARVREGWMAVTADQRPGYAVTTAMNQLVANIRDQKAITGADFLPTMITKENLDQAERVSEAGK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=266, Percent_Identity=32.3308270676692, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790674, Length=226, Percent_Identity=27.4336283185841, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790526, Length=288, Percent_Identity=26.0416666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789990, Length=218, Percent_Identity=25.6880733944954, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789068, Length=203, Percent_Identity=24.6305418719212, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788473, Length=223, Percent_Identity=26.9058295964126, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33191; Mature: 33191

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLILAALIVFTSGVAFAENEQIVFSTPNLAMPFEVHMQRTAVKTAKALGVKLQVLDGQ
CHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCC
GSSPKQVADLENAITRGAQGFIVSPNDINAISSAVEEIQQAKLPVVTLDRSVESEKKVPH
CCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCC
FGANNYKGGEAIANYVKSMFPNGAEIVLLTGQLGSSSNIERTKGIRDSLKTGGDKYRIVV
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
DQTGNWMRSEGMRIIESVLPSLPKPPQVILSANDDMALGAIEALQSHGMKPGEIMVTGFD
ECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCH
AVPEALARVREGWMAVTADQRPGYAVTTAMNQLVANIRDQKAITGADFLPTMITKENLDQ
HHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHH
AERVSEAGK
HHHHHHCCC
>Mature Secondary Structure
MKKLILAALIVFTSGVAFAENEQIVFSTPNLAMPFEVHMQRTAVKTAKALGVKLQVLDGQ
CHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCC
GSSPKQVADLENAITRGAQGFIVSPNDINAISSAVEEIQQAKLPVVTLDRSVESEKKVPH
CCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCC
FGANNYKGGEAIANYVKSMFPNGAEIVLLTGQLGSSSNIERTKGIRDSLKTGGDKYRIVV
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
DQTGNWMRSEGMRIIESVLPSLPKPPQVILSANDDMALGAIEALQSHGMKPGEIMVTGFD
ECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCH
AVPEALARVREGWMAVTADQRPGYAVTTAMNQLVANIRDQKAITGADFLPTMITKENLDQ
HHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHH
AERVSEAGK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]