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The map label for this gene is iolD [H]

Identifier: 22125051

GI number: 22125051

Start: 1294522

End: 1296432

Strand: Direct

Name: iolD [H]

Synonym: y1147

Alternate gene names: 22125051

Gene position: 1294522-1296432 (Clockwise)

Preceding gene: 22125050

Following gene: 22125052

Centisome position: 28.14

GC content: 56.93

Gene sequence:

>1911_bases
ATGACCATGGCACAGGCGCTGGTCAGGTTTCTTAATCAGCAATATGTGGCCATTGATGGTCAGGAGGTCGCATTTGTCCA
CGGCGTGATGACGATTTTCGGGCACGGCAATGTACTCGGTATCGGCCAGGCTCTGGAAGAAAATCCGGGCCATTTACAGG
TGCATCAGGGCTGTAACGAGCAGGGCATGGCGCATATGGCGATTGGTTTTGCGAAACAGTATCGCCGTCAGCGTATCTAT
GCCGTCAGTTCGTCTATCGGGCCGGGAGCCGCCAATATGGTCACCGCAGCGGCCACCGCCACGGCAAACCGCATTCCGCT
GCTGCTATTGCCGGGGGATATCTACGCCAGCCGTCAGCCTGACCCGGTGTTACAGCAGATCGAGCAATATCACGACCTTA
GTATTAGCACTAACGATTGCTTCCGCCCGGTATCACGTTACTGGGATCGCATTAATCGCCCTGAGCAATTGATGAGCGCC
ATGCTGAGTGCTATGCGAGTACTGACTGACCCGGCGGATACCGGTGCGGTGACCCTTTGTCTGCCTCAGGATGTGCAGGG
CGAAGCGTGGGATTACCCGGCGAGCTTCTTTGAGCGCCGGGTGCATCGTATCGAGCGCCGTCCACCGGATACGCGCAGGC
TTTCTGATGCGCTCAGCCTGATCGCCCGTAAACGCCGCCCGCTGGTGGTGTGTGGCGGCGGCGTGCGCTATTCGGGGGCG
CATGAGGCGTTTCGTGCCTTTGTGGAAAACGCTCAATTACCCTTTACTGAAACCCAAGCGGGCAAAGGGGCGCTGCCAAG
TGATCATCCCCTCAACTTGGGGGGAATTGGTGTCACTGGCGGGTTGGCCGCTAACCGGTTAGCTCCACAGGCTGATCTGG
TGATCGGCATCGGTACCCGGCTCACCGATTTCACTACGGGTTCCAAAGCACTATTTTCTCACCCGGAGGTGGCGTTTTTG
CTGCTTAACGTGGCGGAATTCGATGCACTTAAACTGGATGCCACGGCCCTGATTGCCGATGCGCGGGTGGGGCTTGAGGC
ATTAACCGAAGGGCTGGCAGGTTATCGCACTGCTTGGCAGGGCGATATCATTGAGGCCAAAGCAGCGTGGTGCGACGAAT
GTCAACGGCTTTGGTCACGCACCTGGCGGCCTGATGAGGTGCCAGAGGTGGCCGGTCATCTTGATGATTCGCTACAAGAA
TACGCCGAGGTGCTCAATACCACACTGACCCAGACTCGGGTACTTGGGCTTATCAACCAGCATATTGAAGACGATGCCAT
TGTCGTGGGGGCGGCGGGGTCGCTGCCGGGGGATTTACAGCGTCTATGGCAGGTAAAAACGGCAGACACCTATCATCTTG
AATACGGTTATTCATGCATGGGCTATGAAATCGCTGCCGCCATCGGGGCGAAAATTGCGATGCCACATCAACCGGTGTAC
GCCATGGTGGGTGATGGCTCCTATATGATGCTGCATTCCGAGCTGCACACGGCGGTACAAGAGGGGCTAAAAATTACCGT
CCTACTGTTTGATAACGCCGGATTCGGCTGTATCAACAACCTGCAAATGGGCCACGGGATGGGCAGTTTTTGTACCGAGA
GCCGCCACCGTAACCACGAGTCGGGGCAACTCAATGGCCCATTGATTTGCGTTGATTTCGCCCAAAACGCCGAAAGTTAT
GGCTGCCGCGCCTGGCGGGTGCACGATGAACCAAGCCTGCTAGCGGCATTGGCAGCATCCCGCGATCATCCGGGGCCGGT
GCTACTGGATATTAAAGTCTTGCCGAAAAGCATGACGCACGATTATGCGTCTTGGTGGCGTACTGGCGATGCGCAAATCG
CCCGTTCACCCGCGGTACAGCAGGCTGCAGAGCGAACCCAGATGAAGGTAAAACGTGCCCGTCAGTACTAA

Upstream 100 bases:

>100_bases
GAAGCACAGCATGATCAGGGCGCGGCATTCTCTATGCCGACACTCGGTTGAAGGGAGAGGCCGCATGAGCACCGTAAACA
TGGCGATGAAAACAGTACGG

Downstream 100 bases:

>100_bases
CCCTCCGTTATCCTGCCTGTTCATCCGTCGTTGGCTGCAAAAAATGGGGTTAGTTTTAGAAAATGTTATGACTGTGATAG
CAAAACTAACTCACTTTATT

Product: malonic semialdehyde oxidative decarboxylase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNEQGMAHMAIGFAKQYRRQRIY
AVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSA
MLSAMRVLTDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDTRRLSDALSLIARKRRPLVVCGGGVRYSGA
HEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTRLTDFTTGSKALFSHPEVAFL
LLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQE
YAEVLNTTLTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAIGAKIAMPHQPVY
AMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHESGQLNGPLICVDFAQNAESY
GCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY

Sequences:

>Translated_636_residues
MTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNEQGMAHMAIGFAKQYRRQRIY
AVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSA
MLSAMRVLTDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDTRRLSDALSLIARKRRPLVVCGGGVRYSGA
HEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTRLTDFTTGSKALFSHPEVAFL
LLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQE
YAEVLNTTLTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAIGAKIAMPHQPVY
AMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHESGQLNGPLICVDFAQNAESY
GCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY
>Mature_635_residues
TMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNEQGMAHMAIGFAKQYRRQRIYA
VSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSAM
LSAMRVLTDPADTGAVTLCLPQDVQGEAWDYPASFFERRVHRIERRPPDTRRLSDALSLIARKRRPLVVCGGGVRYSGAH
EAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTRLTDFTTGSKALFSHPEVAFLL
LNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQGDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQEY
AEVLNTTLTQTRVLGLINQHIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAIGAKIAMPHQPVYA
MVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHESGQLNGPLICVDFAQNAESYG
CRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTHDYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=22.3801065719361, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI21361361, Length=484, Percent_Identity=23.1404958677686, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1790104, Length=616, Percent_Identity=26.7857142857143, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=27.5045537340619, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=24.7252747252747, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788716, Length=438, Percent_Identity=23.7442922374429, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=653, Percent_Identity=25.2679938744257, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922626, Length=541, Percent_Identity=23.1053604436229, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69644; Mature: 69513

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNE
CCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEECCHHHHCCCCEEEEECCCCC
QGMAHMAIGFAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQP
CCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
DPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSAMLSAMRVLTDPADTGAVTLC
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
LPQDVQGEAWDYPASFFERRVHRIERRPPDTRRLSDALSLIARKRRPLVVCGGGVRYSGA
ECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCH
HEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCC
LTDFTTGSKALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQ
EEECCCCCHHHHCCCCHHHHEEEHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQEYAEVLNTTLTQTRVLGLINQ
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAIGAKIAMPHQPVY
CCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEE
AMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHE
EEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
SGQLNGPLICVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTH
CCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHH
DYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY
HHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMAQALVRFLNQQYVAIDGQEVAFVHGVMTIFGHGNVLGIGQALEENPGHLQVHQGCNE
CHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEECCHHHHCCCCEEEEECCCCC
QGMAHMAIGFAKQYRRQRIYAVSSSIGPGAANMVTAAATATANRIPLLLLPGDIYASRQP
CCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
DPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPEQLMSAMLSAMRVLTDPADTGAVTLC
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEE
LPQDVQGEAWDYPASFFERRVHRIERRPPDTRRLSDALSLIARKRRPLVVCGGGVRYSGA
ECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCH
HEAFRAFVENAQLPFTETQAGKGALPSDHPLNLGGIGVTGGLAANRLAPQADLVIGIGTR
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCC
LTDFTTGSKALFSHPEVAFLLLNVAEFDALKLDATALIADARVGLEALTEGLAGYRTAWQ
EEECCCCCHHHHCCCCHHHHEEEHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDIIEAKAAWCDECQRLWSRTWRPDEVPEVAGHLDDSLQEYAEVLNTTLTQTRVLGLINQ
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIEDDAIVVGAAGSLPGDLQRLWQVKTADTYHLEYGYSCMGYEIAAAIGAKIAMPHQPVY
CCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCEE
AMVGDGSYMMLHSELHTAVQEGLKITVLLFDNAGFGCINNLQMGHGMGSFCTESRHRNHE
EEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
SGQLNGPLICVDFAQNAESYGCRAWRVHDEPSLLAALAASRDHPGPVLLDIKVLPKSMTH
CCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHH
DYASWWRTGDAQIARSPAVQQAAERTQMKVKRARQY
HHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA