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The map label for this gene is pnp [H]

Identifier: 22124608

GI number: 22124608

Start: 778073

End: 780286

Strand: Direct

Name: pnp [H]

Synonym: y0694

Alternate gene names: 22124608

Gene position: 778073-780286 (Clockwise)

Preceding gene: 22124607

Following gene: 22124609

Centisome position: 16.91

GC content: 49.86

Gene sequence:

>2214_bases
TTGTCATTAGTCGCGAGGATGCAGTGTGAAGGTATCAGTAACAGGTGGATTAGATCGTCAATACGATCAGGCACCGCAAA
GCATAAGGGTACAATTTTGCTGACTCCGATTATTCGTAAATTCCAGTATGGTCAACATACCGTAACTATCGAAACTGGTA
TGATGGCTCGCCAGGCCACTGCAGCCGTAATGGTAAGCATGGACGACACTGCAGTATTCGTGACTGTTGTTGGTCAAAAG
AAAGCCAAACCAGGCCAGAGTTTCTTCCCTCTGACGGTTAACTATCAGGAGCGTACTTACGCTGCTGGCCGTATCCCAGG
TAGCTTCTTCCGTCGTGAAGGCCGCCCAAGTGAAGGCGAAACACTGACTTCACGTCTGATCGACCGCCCAATTCGCCCAC
TGTTCCCAGACAGCTTCCTGAACGAAGTTCAAGTGATTGCAACCGTCGTTTCGGTTAACCCACAAATTAACCCAGACATC
GTGGCACTGATTGGTGCATCTGCGGCACTGAGCTTGTCAGGCATTCCATTTAACGGCCCAATCGGTGCTGCTCGCGTTGG
TTTTATCAACGACCAGTATGTGCTGAACCCAACCACTGACGAGCTGAAAGAGAGCCGTTTGGATCTGGTTGTTGCCGGTA
CTGCGGGTGCAGTGCTGATGGTTGAATCAGAAGCCGATATTCTGTCCGAAGAGCAAATGTTGGGCGCGGTCGTCTTCGGC
CACGAACAACAGCAAGTTGTGATTGAAAATATTAACGCTTTGGTTGCTGAAGCTGGCAAACCTAAGTGGGATTGGCAGGC
AGAGCCTGTCAACGAAGCACTGCATGCGCGTGTTGCTGAACTGGCCGAAGCGCGTCTGGGCGATGCATACCGTATCACTG
AAAAACAAGAACGTTATACGCAAGTTGATGCTATCAAAGCTGACGTGACCGAAGCGCTATTAGCGCAGGACGACACATTA
GATGCCGCTGAAATTCAGGATATCCTGGCCAGCGTTGAGAAAAATGTTGTGCGTAGCCGTGTGCTGCGTGGTGAGCCACG
TATCGATGGTCGCGAAAAAGACATGATCCGTGGTTTGGATGTACGTACTGGCATCTTGCCACGTACTCATGGTTCTGCCT
TGTTCACCCGCGGTGAAACTCAGGCGTTGGTTACCGCTACGCTGGGTACGGCACGTGACGCACAAAACATTGATGAGTTG
ATGGGTGAGCGCACAGATTCATTCCTGTTACACTATAATTTCCCTCCGTATTGTGTAGGCGAAACAGGGATGGTTGGCTC
ACCTAAGCGTCGTGAGATTGGCCATGGCCGCCTTGCGAAACGTGGTGTTCTGGCTGTGATGCCAAGTGCGAGCGAGTTCC
CTTACACTATTCGTGTGGTTTCTGAAATCACCGAGTCTAATGGTTCTTCCTCAATGGCTTCTGTTTGTGGTGCTTCTCTG
GCTCTGATGGATGCGGGCGTGCCAATTAAAGCCGCTGTTGCTGGTATTGCAATGGGCCTGGTAAAAGAGGGTGATAACTT
TGTCGTTCTGTCTGACATTCTGGGTGACGAAGATCACTTGGGCGACATGGACTTTAAAGTAGCCGGTAGCCGTGACGGTG
TGACCGCACTGCAAATGGACATTAAAATTGAAGGTATTACCCGCGAAATTATGCAGGTAGCTCTGAATCAGGCTAAGGGT
GCACGTTTGCACATTCTGGGCGTGATGGAACAGGCTATCAGCACACCACGTGGCGATATCTCTGAGTTTGCTCCACGTAT
CTACACCATGAAGATCAACCCTGAGAAAATCAAGGATGTGATCGGTAAAGGTGGTTCGGTGATCCGTGCGCTGACTGATG
AAACTGGCACCACGATTGAAATCGAAGATGATGGTACCATTAAGATTGCAGCAACTGATGGCGATAAAGCAAAACACGCT
ATCCGTCGTATCGAAGAGATTACTGCTGAAATCGAAGTGGGCCGTATTTACGCAGGTAAAGTCACCCGTATCGTTGATTT
CGGTGCATTTGTTGCTATCGGTGGCGGTAAAGAAGGTCTGGTACATATTTCTCAAATCGCTGATAAGCGTGTAGAGAAAG
TGACAGACTACCTGCAGATGGGTCAAGATGTGCCAGTTAAAGTAATGGAAGTTGATCGTCAGGGCCGTATCCGCCTGAGC
ATCAAAGAAGCCACTACTCCTGACGCAGAAGCACCAGAAGCAGCAGCAGAGTAA

Upstream 100 bases:

>100_bases
TTAGTATATTGTTATTATTATATAAGATTTATAAATAGGATCTTCCAATGCAGAGGTTCGCGCGGCTAATGAGAGACTTT
AACTTAATGAAAGTTAAGGA

Downstream 100 bases:

>100_bases
TTAAGTCATTCCACAGCTCCTTGCTCATCGGTAAGGGGCTGTGTTATCTCAGGGGCGGGATGCTCTTGTGTTAAACAAAT
GGATGAAAGGATGTTTATCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 737; Mature: 736

Protein sequence:

>737_residues
MSLVARMQCEGISNRWIRSSIRSGTAKHKGTILLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQK
KAKPGQSFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDI
VALIGASAALSLSGIPFNGPIGAARVGFINDQYVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFG
HEQQQVVIENINALVAEAGKPKWDWQAEPVNEALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTL
DAAEIQDILASVEKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDEL
MGERTDSFLLHYNFPPYCVGETGMVGSPKRREIGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASL
ALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSDILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKG
ARLHILGVMEQAISTPRGDISEFAPRIYTMKINPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHA
IRRIEEITAEIEVGRIYAGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLS
IKEATTPDAEAPEAAAE

Sequences:

>Translated_737_residues
MSLVARMQCEGISNRWIRSSIRSGTAKHKGTILLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQK
KAKPGQSFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDI
VALIGASAALSLSGIPFNGPIGAARVGFINDQYVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFG
HEQQQVVIENINALVAEAGKPKWDWQAEPVNEALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTL
DAAEIQDILASVEKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDEL
MGERTDSFLLHYNFPPYCVGETGMVGSPKRREIGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASL
ALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSDILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKG
ARLHILGVMEQAISTPRGDISEFAPRIYTMKINPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHA
IRRIEEITAEIEVGRIYAGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLS
IKEATTPDAEAPEAAAE
>Mature_736_residues
SLVARMQCEGISNRWIRSSIRSGTAKHKGTILLTPIIRKFQYGQHTVTIETGMMARQATAAVMVSMDDTAVFVTVVGQKK
AKPGQSFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDIV
ALIGASAALSLSGIPFNGPIGAARVGFINDQYVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFGH
EQQQVVIENINALVAEAGKPKWDWQAEPVNEALHARVAELAEARLGDAYRITEKQERYTQVDAIKADVTEALLAQDDTLD
AAEIQDILASVEKNVVRSRVLRGEPRIDGREKDMIRGLDVRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDELM
GERTDSFLLHYNFPPYCVGETGMVGSPKRREIGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASLA
LMDAGVPIKAAVAGIAMGLVKEGDNFVVLSDILGDEDHLGDMDFKVAGSRDGVTALQMDIKIEGITREIMQVALNQAKGA
RLHILGVMEQAISTPRGDISEFAPRIYTMKINPEKIKDVIGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHAI
RRIEEITAEIEVGRIYAGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLSI
KEATTPDAEAPEAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=40.1129943502825, Blast_Score=454, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=89.1774891774892, Blast_Score=1277, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=713, Percent_Identity=34.7826086956522, Blast_Score=351, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=75, Percent_Identity=49.3333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362905, Length=757, Percent_Identity=36.1955085865258, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651641, Length=757, Percent_Identity=36.1955085865258, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651643, Length=757, Percent_Identity=36.1955085865258, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=36.6366366366366, Blast_Score=419, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79736; Mature: 79605

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVARMQCEGISNRWIRSSIRSGTAKHKGTILLTPIIRKFQYGQHTVTIETGMMARQAT
CCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCHHHHCE
AAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGE
EEEEEEECCCEEEEEEECCCCCCCCCCEEEEEEECCHHEEECCCCCHHHHHCCCCCCCCH
TLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDIVALIGASAALSLSGIPFNGP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCEEEECCCCCCCC
IGAARVGFINDQYVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFG
CCCEEEEEECCCEEECCCHHHHHHCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHEEEC
HEQQQVVIENINALVAEAGKPKWDWQAEPVNEALHARVAELAEARLGDAYRITEKQERYT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
QVDAIKADVTEALLAQDDTLDAAEIQDILASVEKNVVRSRVLRGEPRIDGREKDMIRGLD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
VRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDELMGERTDSFLLHYNFPPYCVG
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCEECC
ETGMVGSPKRREIGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASL
CCCCCCCCCHHCCCCCCHHHCCEEEECCCCCCCCEEEEEHHHHHHCCCCHHHHHHHCCHH
ALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSDILGDEDHLGDMDFKVAGSRDGVTALQMD
EEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCEEEECCCCCCEEEEEE
IKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISEFAPRIYTMKINPEKIKDV
EEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCHHHHCCEEEEEEECHHHHHHH
IGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHAIRRIEEITAEIEVGRIYAGK
HCCCCCEEEEEECCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECEEEECC
VTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLS
CEEEEECCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
IKEATTPDAEAPEAAAE
EECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLVARMQCEGISNRWIRSSIRSGTAKHKGTILLTPIIRKFQYGQHTVTIETGMMARQAT
CCCHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHHHHCCCEEEEEECCCHHHHCE
AAVMVSMDDTAVFVTVVGQKKAKPGQSFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGE
EEEEEEECCCEEEEEEECCCCCCCCCCEEEEEEECCHHEEECCCCCHHHHHCCCCCCCCH
TLTSRLIDRPIRPLFPDSFLNEVQVIATVVSVNPQINPDIVALIGASAALSLSGIPFNGP
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEECCCCEEEECCCCCCCC
IGAARVGFINDQYVLNPTTDELKESRLDLVVAGTAGAVLMVESEADILSEEQMLGAVVFG
CCCEEEEEECCCEEECCCHHHHHHCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHEEEC
HEQQQVVIENINALVAEAGKPKWDWQAEPVNEALHARVAELAEARLGDAYRITEKQERYT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
QVDAIKADVTEALLAQDDTLDAAEIQDILASVEKNVVRSRVLRGEPRIDGREKDMIRGLD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
VRTGILPRTHGSALFTRGETQALVTATLGTARDAQNIDELMGERTDSFLLHYNFPPYCVG
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCEECC
ETGMVGSPKRREIGHGRLAKRGVLAVMPSASEFPYTIRVVSEITESNGSSSMASVCGASL
CCCCCCCCCHHCCCCCCHHHCCEEEECCCCCCCCEEEEEHHHHHHCCCCHHHHHHHCCHH
ALMDAGVPIKAAVAGIAMGLVKEGDNFVVLSDILGDEDHLGDMDFKVAGSRDGVTALQMD
EEHCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCEEEECCCCCCEEEEEE
IKIEGITREIMQVALNQAKGARLHILGVMEQAISTPRGDISEFAPRIYTMKINPEKIKDV
EEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCHHHHCCEEEEEEECHHHHHHH
IGKGGSVIRALTDETGTTIEIEDDGTIKIAATDGDKAKHAIRRIEEITAEIEVGRIYAGK
HCCCCCEEEEEECCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECEEEECC
VTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQDVPVKVMEVDRQGRIRLS
CEEEEECCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
IKEATTPDAEAPEAAAE
EECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA