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The map label for this gene is livK [H]

Identifier: 22124337

GI number: 22124337

Start: 470375

End: 471505

Strand: Direct

Name: livK [H]

Synonym: y0422

Alternate gene names: 22124337

Gene position: 470375-471505 (Clockwise)

Preceding gene: 22124335

Following gene: 22124338

Centisome position: 10.22

GC content: 50.22

Gene sequence:

>1131_bases
ATGGGGAATTCGCGGATGAAATTAACAAAAGGTAAAGTGTTGCTGGCTGGGTGTATGGCAATGGCGATGAGTCATTCTGT
GCTCGCGCAAGACATTAAGGTCGCTATTGTGGGGGCAATGTCCGGCCCGGTGGCCCAATACGGTGACATGCAATTTACCG
GTGCGCGCCAGGCTATCGCCGATATCAACGCTAGCGGTGGTATTAAAGGCGATAAGCTGGTTGGTGTGGAATACGATGAT
GCCTGCGATCCGAAACAAGCCGTGGCTGTCGCGAACAAAGTAATAAACGATGGTATCCGTTATGTTATCGGCCATCTGTG
CTCTTCTTCAACTCAGCCAGCTTCAGATATTTATGAAGATGAAGGGGTGATAATGATCACCCCTGCTGCTACAAACGCGG
ATCTGACCACCCGTGGTTACAAAATGATTATGCGCACCACCGGCCTGGATTCAGACCAGGGCCCTACTGCTGCCAAATAT
ATTGTTGAGACGATTAAACCTAAACGTATCGCTGTGGTACATGATAAACAGCAATACGGTGAAGGTCTGGCACGTTCCGT
GCGCGACAGTCTGAAAGAACAAGGTGCAGAGGTTGTGCTGTTTGAAGGTGTGACCGCAGGGGATAAAGATTTCTCGACTC
TGGTTGCTCGCCTGAAGAAAGAGAACGTCGATTTTGTTTATTTCGGCGGTTACTACCCAGAAATGGGCCAAATTCTGCGT
CAGGCTAAGCAAGCAGGCCTAACCGCCCGCTTTATGGGGCCAGAAGGTGTGGGTAACTCCTCACTCTCCAACATCGCGGG
TGAGGCCTCTGAAGGCATGCTGGTGACATTACCTAAGCGTTATGATCAGGTTCCGGCGAACCAACCTATTGTTGATGCGC
TAAAAGCGAAAAAACTGGATCCAACAGGCCCATTTGTGTGGACCACTTATGCTGCGCTGCAATCACTGACGACGGCGATG
GAACGTACTGGCAGCAAAGAACCGGCTGATTTGGCTAACGATCTGAAAACCGGCAAACCGGTTGAGACCGTGATGGGGCC
ATTGAGCTGGGACGATAAAGGCGATTTGAAAGGGTTTGAGTTTGGTATTTTTGAATGGCATGCGGATGGTTCCTCTACCG
CAGTCAAATAA

Upstream 100 bases:

>100_bases
ATAAAGACTTTCAAGGTTAAATCATTATTACTTATGAACAAAATGTTACATTAATGCTAAAACACTCAGACAAACACAAC
AAGCATGTTCACAATGACAG

Downstream 100 bases:

>100_bases
CAAGAATAGTAGAACTATTGACAAGAATAGTAGAACTATCTCGTTTTAGGATTGCTCTCGCAGTAATCCGGCCCCTTCTA
TCAACTGCATCCCCTCGTAT

Product: branched chain amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MGNSRMKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINASGGIKGDKLVGVEYDD
ACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKY
IVETIKPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILR
QAKQAGLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAM
ERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK

Sequences:

>Translated_376_residues
MGNSRMKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINASGGIKGDKLVGVEYDD
ACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKY
IVETIKPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILR
QAKQAGLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAM
ERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK
>Mature_375_residues
GNSRMKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIADINASGGIKGDKLVGVEYDDA
CDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYEDEGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYI
VETIKPKRIAVVHDKQQYGEGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILRQ
AKQAGLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLDPTGPFVWTTYAALQSLTTAME
RTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFEFGIFEWHADGSSTAVK

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=75.3424657534247, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=74.6594005449591, Blast_Score=583, Evalue=1e-168,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40176; Mature: 40045

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSRMKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIA
CCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCEEECCHHHHHH
DINASGGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYED
HCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
EGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETIKPKRIAVVHDKQQYG
CCEEEEECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHH
EGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILR
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
QAKQAGLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLD
HHHHCCCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCC
PTGPFVWTTYAALQSLTTAMERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFE
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEE
FGIFEWHADGSSTAVK
EEEEEEECCCCCCCCC
>Mature Secondary Structure 
GNSRMKLTKGKVLLAGCMAMAMSHSVLAQDIKVAIVGAMSGPVAQYGDMQFTGARQAIA
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCEEECCHHHHHH
DINASGGIKGDKLVGVEYDDACDPKQAVAVANKVINDGIRYVIGHLCSSSTQPASDIYED
HCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
EGVIMITPAATNADLTTRGYKMIMRTTGLDSDQGPTAAKYIVETIKPKRIAVVHDKQQYG
CCEEEEECCCCCCCCCHHHHHEEHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHH
EGLARSVRDSLKEQGAEVVLFEGVTAGDKDFSTLVARLKKENVDFVYFGGYYPEMGQILR
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
QAKQAGLTARFMGPEGVGNSSLSNIAGEASEGMLVTLPKRYDQVPANQPIVDALKAKKLD
HHHHCCCEEEECCCCCCCCCHHHHHCCCCCCCEEEECCHHHCCCCCCCCHHHHHHHCCCC
PTGPFVWTTYAALQSLTTAMERTGSKEPADLANDLKTGKPVETVMGPLSWDDKGDLKGFE
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEE
FGIFEWHADGSSTAVK
EEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]