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The map label for this gene is uvrD [H]

Identifier: 22124304

GI number: 22124304

Start: 433472

End: 435640

Strand: Direct

Name: uvrD [H]

Synonym: y0389

Alternate gene names: 22124304

Gene position: 433472-435640 (Clockwise)

Preceding gene: 22124303

Following gene: 22124306

Centisome position: 9.42

GC content: 54.59

Gene sequence:

>2169_bases
GTGCCTATGGACGTTTCTGATCTGCTCGACAGCCTGAATGAAAAACAACGTGACGCCGTGGCCGCGCCGCGCGGCAACTT
GTTGGTACTGGCCGGTGCAGGCAGTGGCAAAACGCGGGTGCTGGTTCACCGTATCGCCTGGCTGTTGTCGGTAGAGAATG
CCTCGCCGTATTCGATTATCTCGGTAACCTTTACCAATAAAGCGGCGGCTGAAATGCGCCACCGTATTGAACATCTGATT
GGCACCAGCCAAGGCGGGATGTGGATCGGGACTTTCCACGGTTTGGCGCACCGTCTGCTACGTGCTCACCATCTGGATGC
CAATCTGCCACAAGATTTTCAAATCCTGGATAGCGATGACCAACTGCGGTTATTGAAACGCCTGGTCAAAGCACTCAATT
TGGATGAGAAACAATGGCCCCCGCGTCAGGCGATGTGGTACATCAACGGCAAAAAAGACGAAGGGCTGCGCCCGCAACAT
ATTGAGAGCTACGGTAATCCGGTTGAGGCCACCTGGCTACGTATCTATCAGGCTTATCAGGAAGCGTGCGACCGCGCGGG
TCTGGTTGATTTTGCCGAGCTGTTGCTACGAGCGCACGAGCTGTGGCTGAACAAACCACATATTCTTAATCATTATCGTG
AACGCTTTACCAACATTTTGGTGGATGAGTTTCAGGATACCAACAACATCCAATATGCCTGGATCCGCTTGTTGGCCGGT
GAACGTTCCAATGTGATGATCGTCGGCGACGATGACCAGTCAATCTATGGTTGGCGCGGCGCGCAGGTCGAGAATATCCA
ACGTTTCTTGAAGGATTTCCCCGGTGCGGAGACCATCCGCCTGGAGCAGAATTACCGCTCGACCAGCATCATTCTGGCTG
CCGCCAATGCCCTGATTGCCAACAATGACGGCCGCATGGGTAAAAATCTGTGGACCGATGGCGCGGAGGGCGAACCTATC
TCCCTCTATTGTGCGTTTAACGAGTTAGATGAAGCCCGTTTTGTGGTCAATCGTATCAAAGCCTGGCAGGACAACGGCGG
TGCGCTGAATGATTGTGCCATTTTGTACCGTAGCAACGCCCAATCGCGCGTATTAGAAGAAGCGTTACTGCAAACGGCAA
CCCCGTACCGTATTTATGGGGGGCAGCGCTTCTTCGACCGCCAGGAAATTAAAGACTCACTGGCTTATCTGCGCCTGATC
TCCAATCGTAATGACGACGCCGCGTTTGAACGGGTGGTGAATACACCCACTCGGGGGATTGGCGATCGCACATTGGATGT
GGTGCGTCAAACCGCCCGTGACCGCCAGTTAACGCTATGGCAATCAACCCGTGCCATGTTGCAGGAAAAAGTGCTGGCAG
GGCGTGCCGCGTCCGCATTACAACGCTTTGTCGAGCTGATCGATTCACTGGCGCGCGAAACGGCTGATATGCCGCTACAC
GTGCAAACTGATCGCGTTATTCGTGATTCTGGCTTGTGGTCGATGTACGAACAGGAAAAAGGCGAGAAAGGTCAGGCACG
TATCGAGAACCTTGAAGAGCTGGTTACGGCGACCCGTCAATACAGCTATCAGGATGAAGATCAGGATCTGATGCCGCTAC
AGGCCTTCTTATCCCATGCGGCACTGGAAGCCGGAGATGGCCAGGCCGATGCCTATCAGGATGCGGTGCAATTAATGACT
CTCCATTCAGCGAAAGGCTTGGAGTTTCCACAGGTATTTATTGTGGGAATGGAAGAAGGGATGTTCCCGAGCCAAATGTC
ATTGGATGAGGGGGGGCGTTTGGAAGAGGAGCGGCGTTTGGCCTACGTGGGGTTAACCCGTGCGATGCAGAAACTGACGT
TATGTTACGCCGAAAGCCGCCGCCTATACGGTAAAGAGGTCAATCACCGCCCTTCACGCTTTATCGGCGAACTGCCGCAG
GAGTGCGTCGAAGAGGTCCGGCTGCGCGCCAGTGTTTCCCGCCCGGTGAATCACCGCCGAATGGGGACTCCGATGCACGA
GAATGACAGCGGTTTCTCATTGGGCCAGCGAGTGCGGCACCCTAAATTTGGCGAGGGTACTATTGTGAATTTGGAAGGCA
GTGGTGAGCACAACCGGTTGCAGGTTGCCTTCCCCGGAGAGGGGATTAAGTGGCTGGTCGCGGCCTATGCGCGTCTGGAA
CCCGTGTAA

Upstream 100 bases:

>100_bases
TATAATCCTCAGCAATAACCCTGTGTAAATCTACAGTGGGAGGGGGGCAAACTACCGGAGACCCGGGGCATTGTGGCTAT
TTTCCCTTACCCGATAAACG

Downstream 100 bases:

>100_bases
TCTGATATTTGTGGTGGTTGCAGTGTCAGTGCGGCCACCTATCTATTTTTATTTCTCCTTACTATCTTTATTGCTCCTTA
CTATTTTCATTGTTCTTACT

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 722; Mature: 721

Protein sequence:

>722_residues
MPMDVSDLLDSLNEKQRDAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENASPYSIISVTFTNKAAAEMRHRIEHLI
GTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDDQLRLLKRLVKALNLDEKQWPPRQAMWYINGKKDEGLRPQH
IESYGNPVEATWLRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNILVDEFQDTNNIQYAWIRLLAG
ERSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAETIRLEQNYRSTSIILAAANALIANNDGRMGKNLWTDGAEGEPI
SLYCAFNELDEARFVVNRIKAWQDNGGALNDCAILYRSNAQSRVLEEALLQTATPYRIYGGQRFFDRQEIKDSLAYLRLI
SNRNDDAAFERVVNTPTRGIGDRTLDVVRQTARDRQLTLWQSTRAMLQEKVLAGRAASALQRFVELIDSLARETADMPLH
VQTDRVIRDSGLWSMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHAALEAGDGQADAYQDAVQLMT
LHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGLTRAMQKLTLCYAESRRLYGKEVNHRPSRFIGELPQ
ECVEEVRLRASVSRPVNHRRMGTPMHENDSGFSLGQRVRHPKFGEGTIVNLEGSGEHNRLQVAFPGEGIKWLVAAYARLE
PV

Sequences:

>Translated_722_residues
MPMDVSDLLDSLNEKQRDAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENASPYSIISVTFTNKAAAEMRHRIEHLI
GTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDDQLRLLKRLVKALNLDEKQWPPRQAMWYINGKKDEGLRPQH
IESYGNPVEATWLRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNILVDEFQDTNNIQYAWIRLLAG
ERSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAETIRLEQNYRSTSIILAAANALIANNDGRMGKNLWTDGAEGEPI
SLYCAFNELDEARFVVNRIKAWQDNGGALNDCAILYRSNAQSRVLEEALLQTATPYRIYGGQRFFDRQEIKDSLAYLRLI
SNRNDDAAFERVVNTPTRGIGDRTLDVVRQTARDRQLTLWQSTRAMLQEKVLAGRAASALQRFVELIDSLARETADMPLH
VQTDRVIRDSGLWSMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHAALEAGDGQADAYQDAVQLMT
LHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGLTRAMQKLTLCYAESRRLYGKEVNHRPSRFIGELPQ
ECVEEVRLRASVSRPVNHRRMGTPMHENDSGFSLGQRVRHPKFGEGTIVNLEGSGEHNRLQVAFPGEGIKWLVAAYARLE
PV
>Mature_721_residues
PMDVSDLLDSLNEKQRDAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENASPYSIISVTFTNKAAAEMRHRIEHLIG
TSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDDQLRLLKRLVKALNLDEKQWPPRQAMWYINGKKDEGLRPQHI
ESYGNPVEATWLRIYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNILVDEFQDTNNIQYAWIRLLAGE
RSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAETIRLEQNYRSTSIILAAANALIANNDGRMGKNLWTDGAEGEPIS
LYCAFNELDEARFVVNRIKAWQDNGGALNDCAILYRSNAQSRVLEEALLQTATPYRIYGGQRFFDRQEIKDSLAYLRLIS
NRNDDAAFERVVNTPTRGIGDRTLDVVRQTARDRQLTLWQSTRAMLQEKVLAGRAASALQRFVELIDSLARETADMPLHV
QTDRVIRDSGLWSMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHAALEAGDGQADAYQDAVQLMTL
HSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGLTRAMQKLTLCYAESRRLYGKEVNHRPSRFIGELPQE
CVEEVRLRASVSRPVNHRRMGTPMHENDSGFSLGQRVRHPKFGEGTIVNLEGSGEHNRLQVAFPGEGIKWLVAAYARLEP
V

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=720, Percent_Identity=88.1944444444444, Blast_Score=1337, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=640, Percent_Identity=39.375, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=28.8235294117647, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=799, Percent_Identity=27.909887359199, Blast_Score=186, Evalue=9e-48,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81994; Mature: 81863

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMDVSDLLDSLNEKQRDAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENASPYSII
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEE
SVTFTNKAAAEMRHRIEHLIGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDD
EEEECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
QLRLLKRLVKALNLDEKQWPPRQAMWYINGKKDEGLRPQHIESYGNPVEATWLRIYQAYQ
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
EACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNILVDEFQDTNNIQYAWIRLLAG
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHEEECC
ERSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAETIRLEQNYRSTSIILAAANALIA
CCCCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCEE
NNDGRMGKNLWTDGAEGEPISLYCAFNELDEARFVVNRIKAWQDNGGALNDCAILYRSNA
CCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
QSRVLEEALLQTATPYRIYGGQRFFDRQEIKDSLAYLRLISNRNDDAAFERVVNTPTRGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
GDRTLDVVRQTARDRQLTLWQSTRAMLQEKVLAGRAASALQRFVELIDSLARETADMPLH
CHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VQTDRVIRDSGLWSMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHA
EECCHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ALEAGDGQADAYQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRL
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
AYVGLTRAMQKLTLCYAESRRLYGKEVNHRPSRFIGELPQECVEEVRLRASVSRPVNHRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
MGTPMHENDSGFSLGQRVRHPKFGEGTIVNLEGSGEHNRLQVAFPGEGIKWLVAAYARLE
CCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHC
PV
CC
>Mature Secondary Structure 
PMDVSDLLDSLNEKQRDAVAAPRGNLLVLAGAGSGKTRVLVHRIAWLLSVENASPYSII
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEE
SVTFTNKAAAEMRHRIEHLIGTSQGGMWIGTFHGLAHRLLRAHHLDANLPQDFQILDSDD
EEEECCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
QLRLLKRLVKALNLDEKQWPPRQAMWYINGKKDEGLRPQHIESYGNPVEATWLRIYQAYQ
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
EACDRAGLVDFAELLLRAHELWLNKPHILNHYRERFTNILVDEFQDTNNIQYAWIRLLAG
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHEEECC
ERSNVMIVGDDDQSIYGWRGAQVENIQRFLKDFPGAETIRLEQNYRSTSIILAAANALIA
CCCCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCEE
NNDGRMGKNLWTDGAEGEPISLYCAFNELDEARFVVNRIKAWQDNGGALNDCAILYRSNA
CCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
QSRVLEEALLQTATPYRIYGGQRFFDRQEIKDSLAYLRLISNRNDDAAFERVVNTPTRGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
GDRTLDVVRQTARDRQLTLWQSTRAMLQEKVLAGRAASALQRFVELIDSLARETADMPLH
CHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VQTDRVIRDSGLWSMYEQEKGEKGQARIENLEELVTATRQYSYQDEDQDLMPLQAFLSHA
EECCHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ALEAGDGQADAYQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRL
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
AYVGLTRAMQKLTLCYAESRRLYGKEVNHRPSRFIGELPQECVEEVRLRASVSRPVNHRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
MGTPMHENDSGFSLGQRVRHPKFGEGTIVNLEGSGEHNRLQVAFPGEGIKWLVAAYARLE
CCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHC
PV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]