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The map label for this gene is rep [H]

Identifier: 22124272

GI number: 22124272

Start: 396714

End: 398789

Strand: Direct

Name: rep [H]

Synonym: y0356

Alternate gene names: 22124272

Gene position: 396714-398789 (Clockwise)

Preceding gene: 22124263

Following gene: 22124275

Centisome position: 8.62

GC content: 48.84

Gene sequence:

>2076_bases
GTGGCTTTCTGGTACAATTCGCTTCCCCGTAAACCCATTGAGCAACCGAGCGTCATGCGATTAAACCCCAGCCAACAAGA
AGCCGTCGAATTTGTCACTGGACCCTGTCTGGTGCTGGCCGGTGCGGGATCGGGTAAAACCCGAGTCATTACCAACAAGA
TTGCCCACCTGATCCGTCAGTGTGGTTATCAGCCTAAGCATATTGCTGCCGTGACCTTCACCAATAAAGCAGCCCGCGAG
ATGAAAGAGCGTGTTGCTCAAACGCTGGGGCGCAAAGAAGCGCGGGGATTGATGATCGCGACTTTCCACACCTTGGGGTT
GGAAATCATCAAGAAAGAGTATGTGGCTCTGGGGATGAAGTCGAACTTCTCACTGTTCGATGCACAGGATCAAATGGGGT
TATTGAAAGATCTGACCCATAAGTGGCTGGAAGATGACAAAACATTGCTGCAGCAACTGGTGTCGGCGATCTCCAATTGG
AAGAATGATCTGCTGGATCCAGCCGCCGCTGCTGCGACAGCACGATCCGAGCGTGATAAGTTATTTGTCCATTGCTACGG
CTTATATGACGCCCATTTGAAAGCCTGTAATGTGCTGGATTTTGACGACCTTATCTCGCTACCCACCCTATTGTTGCAAA
AAAATCTGGAGGTCCGTGAGCGGTGGCAAAACCGTCTGCGTTACCTGCTGGTTGATGAATATCAGGATACCAATACCAGC
CAGTATCAAATGGTGAAATTGCTGGTGGGGAATCGGGCGCGTTTTACTGTCGTGGGTGATGACGACCAATCTATCTATTC
ATGGCGTGGTGCTCGGCCACAGAACTTGGTCCTGCTAAATGAAGATTTCCCTGCGCTACGGGTGATTAAGCTGGAACAGA
ATTACCGTTCTTCGGGGCGTATCTTGAAGGCGGCGAATATTTTGATTGCCAACAACCCACATGTTTTTGAAAAGAAACTT
TTTTCTGAACTGAGCTATGGCGATGAGTTGAAAGTGATTACCGCCAATAACGAAGATCATGAAGCGGAGCGGGTGGTCGG
CGAGCTTATTGCACATCACTTTGTTAAAAAGACCCAATATAGCGATTACGCCATTTTGTATCGTGGGAATCACCAATCGC
GGTTGTTTGAAAAATTGCTGATGCAAAACCGTATTCCTTATCGAATTTCGGGTGGCGATTCATTTTTCTCTCGTCCAGAG
ATTAAGGATTTGTTGGCTTACCTACGGGTATTGACCAATCAGGATGATGACAGTGCATTCTTACGAATTGTGAATACACC
AAAGCGTGAGATTGGTGCGGCCACCGTCCAAAAATTGGGTGAATGGGCTAATTTACGCAATAAGAGCCTGTTTCGGGCCA
GTTTTGATTTAGGGTTAGGCGAGCACCTGAAGGGCCGTGGCCTGGAGTCATTGCAACGTTTTACTCACTGGATGGATGGG
ATTATTCGTTTGGTTGAGCGTGAACCCGTGGCAGCAGTCCGGGATTTGATCCACGGTATTGATTACGAAAGTTGGTTATT
CGAAACTGCGCCCAGCCCAAAAGCCGCTGAAATGCGTATGAAAAACGTTAATTTATTATTCAGTTGGATGACCGAAATGC
TGGAAGGGTCGGAACTGGATGAACCGATGACGCTGACTCAGGTGGTAACGCGTTTTACCCTGCGTGACATGATGGAGCGA
GGGGAGAGTGATGAAGAACTGGATCAAGTGCAATTAATGACGTTGCATGCCTCTAAAGGGCTGGAGTTCCCTTATGTATT
CTTAGTGGGGATGGAAGAGGGGTTGCTGCCGCATCAAAGCAGCATTGATGAAGATAATGTGGATGAAGAACGGCGGTTGG
CTTATGTGGGGATCACCCGCGCCCAGCGTGAACTGTTCTTCACCCTGTGTAAAGAACGGCGGCAATACGGCGAGTTGATT
CGGCCAGAACCTAGCCGTTTTCTGATGGAGTTGCCGCAGGACGATTTAAACTGGGAAAACGAACGTAAAGCCGTCAGCCC
GGAAGAGCGTATGCAAAAAGGCCAGAGTCATCTGGCCAATCTTCGTGCTCAGTTAGCGAATGCGAAGAAACCGTAG

Upstream 100 bases:

>100_bases
AGTCTCTTCGATAGGCTCGCGGAGCGCGTCAGTGTAACGTATTTTGGTTGCCGCTGCGTGCGTATTGCCAATCAAAAAAG
CAGGCAGTTTTTTTTCATCT

Downstream 100 bases:

>100_bases
TTAATATGAAAAGCCGTAGTTAACACGAAAAGCAGCAGTTACAGTAGTGAAGATAATAACGGGCAGTCTATTGCCCGTTA
GCCATGCATTAAGGCGCGAC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MAFWYNSLPRKPIEQPSVMRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAARE
MKERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNW
KNDLLDPAAAAATARSERDKLFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTS
QYQMVKLLVGNRARFTVVGDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEKKL
FSELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPE
IKDLLAYLRVLTNQDDDSAFLRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDG
IIRLVEREPVAAVRDLIHGIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMER
GESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELI
RPEPSRFLMELPQDDLNWENERKAVSPEERMQKGQSHLANLRAQLANAKKP

Sequences:

>Translated_691_residues
MAFWYNSLPRKPIEQPSVMRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAARE
MKERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNW
KNDLLDPAAAAATARSERDKLFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTS
QYQMVKLLVGNRARFTVVGDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEKKL
FSELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPE
IKDLLAYLRVLTNQDDDSAFLRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDG
IIRLVEREPVAAVRDLIHGIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMER
GESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELI
RPEPSRFLMELPQDDLNWENERKAVSPEERMQKGQSHLANLRAQLANAKKP
>Mature_690_residues
AFWYNSLPRKPIEQPSVMRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAAREM
KERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNWK
NDLLDPAAAAATARSERDKLFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTSQ
YQMVKLLVGNRARFTVVGDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEKKLF
SELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPEI
KDLLAYLRVLTNQDDDSAFLRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDGI
IRLVEREPVAAVRDLIHGIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERG
ESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELIR
PEPSRFLMELPQDDLNWENERKAVSPEERMQKGQSHLANLRAQLANAKKP

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=85.0299401197605, Blast_Score=1187, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=660, Percent_Identity=38.1818181818182, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=359, Percent_Identity=29.2479108635098, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=736, Percent_Identity=27.1739130434783, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=696, Percent_Identity=21.9827586206897, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79463; Mature: 79332

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFWYNSLPRKPIEQPSVMRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQ
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
CGYQPKHIAAVTFTNKAAREMKERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMK
CCCCCCEEEEEEECCHHHHHHHHHHHHHHCHHHHCCEEEEHHHHHHHHHHHHHHHHHCCC
SNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSERDK
CCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCE
LFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTS
EEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCH
QYQMVKLLVGNRARFTVVGDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGR
HHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCC
ILKAANILIANNPHVFEKKLFSELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQY
EEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDSAF
CCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCE
LRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDG
EEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHH
IIRLVEREPVAAVRDLIHGIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELD
HHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQS
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCCCCC
SIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNWEN
CCCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
ERKAVSPEERMQKGQSHLANLRAQLANAKKP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AFWYNSLPRKPIEQPSVMRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQ
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
CGYQPKHIAAVTFTNKAAREMKERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMK
CCCCCCEEEEEEECCHHHHHHHHHHHHHHCHHHHCCEEEEHHHHHHHHHHHHHHHHHCCC
SNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSERDK
CCCCEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCE
LFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTS
EEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCH
QYQMVKLLVGNRARFTVVGDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGR
HHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCC
ILKAANILIANNPHVFEKKLFSELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQY
EEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDSAF
CCEEEEEECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCE
LRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDG
EEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHH
IIRLVEREPVAAVRDLIHGIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELD
HHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQS
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCCCCEEEEEECCCCCCCCCC
SIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNWEN
CCCCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
ERKAVSPEERMQKGQSHLANLRAQLANAKKP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]