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The map label for this gene is cysJ [H]

Identifier: 161484862

GI number: 161484862

Start: 916831

End: 918669

Strand: Direct

Name: cysJ [H]

Synonym: y0818

Alternate gene names: 161484862

Gene position: 916831-918669 (Clockwise)

Preceding gene: 22124729

Following gene: 22124732

Centisome position: 19.93

GC content: 52.2

Gene sequence:

>1839_bases
ATGACGACTCAGGCTCCTCCAACTTCGTTACTTCCGCTATCGCCAGAGCAGTTGGCCCGCTTGCAAGCGGCGGTGGGTGA
ATTCTCCCCAACCCAAATGGCATGGTTGTCGGGTTACTTCTGGGGGATGGTTAACCAGCAGCCCGGCGCTGTTGCTTCCC
CTGCGGTAGCGGCACCCCCACCAGTGACAGTTACCCTGATTTCCGCGTCTCAAACCGGCAACGCCCGGCGTTTAGCTGAA
CAACTGCGCGATGATCTTCTGGCTGCCCAACTTAGTGTCAATCTGGTCAATGCGGGTGATTATAAGTTTAAACAAATTGC
GCAGGAGCGTTTACTGGTGGTGGTCGCATCGACCCAAGGAGAGGGGGAGCCAGCAGAAGAAGCCGTCGCACTGCATAAAT
TCCTGTTTTCTAAAAAAGCCCCAAAATTATCGGAGACCGCATTTGCTGTCTTTGGCTTAGGTGACACCTCTTACGAACAC
TTCTGTCAGGCTGGGAAAGATTTTGATAGCAAGCTGGCAGAACTTGGTGCTCAACGGTTACTGGATCGTGTCGATGCCGA
TGTTGAATATCAGGTACAAGCACAGCAGTGGCGTCAGCAAGTGGTTGCCACGTTGCAGGCTAAGGTTCCAGCACAATCTA
CAGCTCCAACACAATTTATAGCTCCAACACAATCTACAACTCCAGCAGCAGCGGCGATAACGTCTGGTGGTACCACTACC
GTTAGTCCGTACAGTAAAACCGCGCCGTTGACCGCACAGTTATCCGTACAGCAAAAAGTGACTGGGCGTAATTCAGAAAA
AGATGTTCGTCATATTGAAATCGATTTAGGTGATTCAGGACTGCGTTACCAACCAGGCGATGCTTTGGGTGTCTGGTTTG
ATAATGATCCTGCTTTGGTCGAAGAACTGCTCGCGCTGTTGTGGCTCAAAGGTGATGAGCCGGTCAGTATTGACGGACAA
AATATGCCACTGGCACAGGCGCTGCTCAGTCACCTTGAACTGACACAAAATACGACGCTGATCGTCGATAAATACGCCGC
GCTATCACGCGATGAAACCTTGATTGCACTGCTGGCAGATAAACCCGCGTTGCAACTGTACGCTAAGAATACGCCATTCG
TTGATATGGTGCGGCAGGCACCGTCTGATCTTAATGCCGATCAACTGGTGGGGTTATTGCGCCCCTTAACGCCACGGTTG
TACTCCATCGCCTCTTCGCAAGCTGAGACCGAAAATGAGGTGCATATCACCGTGGGTGTGGTGCGCTATGACATTGATGG
CCGAGCGCGCAGTGGCGGTGCTTCCGGCTATCTGGCGGACCGGCTGGAAGTCGATGGCGATATCCGTGTCTTTATTGAAC
ACAATGATAATTTCCGTTTGCCTGCTAACCCGGAAACACCCGTCATCATGATCGGGCCAGGCACCGGTATCGCGCCGTTC
CGCGCCTTTATGCAACAGCGTGAAGTTGATGGGGCCAGTGGCAAGAACTGGTTATTCTTCGGTAATCCTCATTTCACCGA
GGATTTTCTGTATCAGGTTGAATGGCAACGCTATGTCAAAGAAGGCGTGTTGACGCGCATTGATCTGGCCTGGTCCCGTG
ATCAGGCGCATAAAATATACGTACAAGACAAACTGCGCGAACAGGGCGCGGAACTGTGGAACTGGATACAACAAGGTGCC
CACATTTACGTCTGTGGCGATGCTAACCGCATGGCGAAAGACGTTGAGCAGGTTTTACTGGACGTGGTGGCTCTGCACGG
TGCAATGGATGCCGAGCAGGCTGATGAATATTTAAGTGAGTTGCGCCAAGCGCGCCGCTATCAGCGAGATGTGTACTGA

Upstream 100 bases:

>100_bases
ACCTTACAAACTATCAAGACTTTGTGGCACGAATTGTCCTGCCTATATTGACAAATATTTGTGCCAACCACGGCTAAAGC
ATAAGGGAATTCGTACTGCA

Downstream 100 bases:

>100_bases
TGAATGAAAAACACCCAGGGCCACTCGTCGTCAGTGGAAAACTTTCTGATGGCGAGCGCATGAAGTCGGAAAGCAATTTC
TTGCGTGGGACTATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAE
QLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEH
FCQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQSTTPAAAAITSGGTTT
VSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPVSIDGQ
NMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRL
YSIASSQAETENEVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPF
RAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNWIQQGA
HIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSELRQARRYQRDVY

Sequences:

>Translated_612_residues
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAE
QLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEH
FCQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQSTTPAAAAITSGGTTT
VSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPVSIDGQ
NMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRL
YSIASSQAETENEVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPF
RAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNWIQQGA
HIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSELRQARRYQRDVY
>Mature_611_residues
TTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAEQ
LRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEHF
CQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQSTTPAAAAITSGGTTTV
SPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPVSIDGQN
MPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLY
SIASSQAETENEVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFR
AFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNWIQQGAH
IYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSELRQARRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=602, Percent_Identity=31.3953488372093, Blast_Score=256, Evalue=5e-68,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=32.2742474916388, Blast_Score=233, Evalue=6e-61,
Organism=Homo sapiens, GI7657393, Length=597, Percent_Identity=29.6482412060301, Blast_Score=217, Evalue=3e-56,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=29.8157453936348, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI221316705, Length=607, Percent_Identity=29.3245469522241, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI10835173, Length=652, Percent_Identity=28.8343558282209, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI221316707, Length=539, Percent_Identity=29.4990723562152, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI40254422, Length=651, Percent_Identity=27.9569892473118, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI169790956, Length=446, Percent_Identity=27.8026905829596, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI169790958, Length=446, Percent_Identity=27.8026905829596, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1789123, Length=612, Percent_Identity=73.202614379085, Blast_Score=920, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=639, Percent_Identity=29.4209702660407, Blast_Score=249, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=576, Percent_Identity=25, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531441, Length=395, Percent_Identity=26.5822784810127, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321832, Length=639, Percent_Identity=27.8560250391236, Blast_Score=201, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321143, Length=394, Percent_Identity=31.7258883248731, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325305, Length=627, Percent_Identity=23.2854864433812, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137192, Length=601, Percent_Identity=29.1181364392679, Blast_Score=223, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24582192, Length=511, Percent_Identity=30.3326810176125, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24583543, Length=535, Percent_Identity=30.2803738317757, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI78706876, Length=535, Percent_Identity=30.2803738317757, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI78706872, Length=535, Percent_Identity=30.2803738317757, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24660907, Length=594, Percent_Identity=26.2626262626263, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24660903, Length=594, Percent_Identity=26.2626262626263, Blast_Score=142, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 67284; Mature: 67152

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCC
PVTVTLISASQTGNARRLAEQLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCEEEEEEECCC
EGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEHFCQAGKDFDSKLAELGAQRL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
LDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQSTTPAAAAITSGGTTT
HHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHEEECCCCEE
VSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALV
ECCCCCCCCCEEEEEHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHH
EELLALLWLKGDEPVSIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLAD
HHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHCCCCCEEEEEEEC
KPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENEVHITVGV
CCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEE
VRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPF
EEEECCCCCCCCCCCCEEEHHEECCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHH
RAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIY
HHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEE
VQDKLREQGAELWNWIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSE
EHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRQARRYQRDVY
HHHHHHHHHCCC
>Mature Secondary Structure 
TTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPP
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCC
PVTVTLISASQTGNARRLAEQLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCEEEEEEECCC
EGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEHFCQAGKDFDSKLAELGAQRL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
LDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQSTTPAAAAITSGGTTT
HHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHEEECCCCEE
VSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALV
ECCCCCCCCCEEEEEHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHH
EELLALLWLKGDEPVSIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLAD
HHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHCCCCCEEEEEEEC
KPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENEVHITVGV
CCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEE
VRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPF
EEEECCCCCCCCCCCCEEEHHEECCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHH
RAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIY
HHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEE
VQDKLREQGAELWNWIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSE
EHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRQARRYQRDVY
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA