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The map label for this gene is pfl [H]

Identifier: 21911132

GI number: 21911132

Start: 1596440

End: 1598767

Strand: Direct

Name: pfl [H]

Synonym: SpyM3_1596

Alternate gene names: 21911132

Gene position: 1596440-1598767 (Clockwise)

Preceding gene: 21911130

Following gene: 21911135

Centisome position: 84.0

GC content: 41.84

Gene sequence:

>2328_bases
ATGGCAACTGTTAAAACCAATACAGATGTTTTCGAAAAAGCTTGGGAAGGCTTTAAAGGAACTGACTGGAAAGAAAAAGC
AAGTGTCTCACGTTTTGTACAAGCTAACTACACCCCATACGATGGCGATGAATCTTTCTTGGCTGGTGCTACTGAACGTT
CACTTCACATCAAAAAAGTTATCGAAGAAACAAAAGCACACTATGAAGCTACACGTTTCCCATACGATACTCGTCCAACT
TCAATTGCTGACATTCCTGCTGGTTTCATCGATAAAGAAAATGAATTAATTTATGGTATTCAAAACGATGAATTGTTCAA
ATTGAACTTCATGCCAAAAGGTGGTATCCGTATGGCGGAAACGACCCTTAAAGAAAACGGCTATGAACCAGACCCAGCAG
TACATGAAATCTTTACTAAGTATGTGACAACTGTTAACGACGGTATCTTCCGCGCTTATACTTCAAATATTCGTCGCGCT
CGTCATGCCCACACTGTAACTGGTCTTCCAGATGCTTACTCACGTGGACGTATCATCGGTGTTTACGCTCGTCTTGCTCT
TTACGGTGCTGACTACTTGATGCAAGAAAAAGTCAATGACTGGAATGCTATCACTGAAATCGATGAAGAATCCATTCGTC
TTCGCGAAGAAGTTAACCTTCAATACCAAGCTCTTGGTGAAGTGGTTAAACTTGGTGACCTTTACGGAGTTGATGTTCGT
CGCCCAGCACAAAACGTTAAAGAAGCTATCCAATGGGTAAACATCGCTTTCATGGCTGTATGTCGTGTGATCAACGGTGC
TGCTACTTCTCTTGGACGTGTGCCAATCGTTCTTGATATCTTTGCAGAACGTGACCTTGCTCGCGGTACCTTTACAGAAT
CAGAAATCCAAGAATTTGTTGATGATTTTGTCCTTAAACTCCGTACCGTAAAATTTGGTCGTACGAAAGCTTACGATGCG
CTTTACTCAGGTGACCCAACATTCATCACAACTTCTATGGCTGGTATGGGTAACGATGGTCGTCACCGTGTAACTAAGAT
GGACTACCGTTTCTTGAACACGCTTGATAATATTGGTAACTCTCCAGAACCAAACTTGACTGTTCTTTGGACTGACCAAC
TGCCAGAAACATTCCGTCGCTACTGTATGAAAATGAGCCACAAGCACTCTTCTATCCAATATGAAGGTGTGACAACAATG
GCTAAAGAAGGTTATGGTGAAATGTCATGTATTTCATGTTGTGTGTCACCACTTGACCCAGAAAATGAAGAACAACGTCA
TAACATCCAATACTTCGGTGCTCGTGTAAACGTTCTTAAAGCCCTTCTTACTGGTCTTAACGGTGGTTACGATGATGTTC
ACAGAAACTACAAAGTGTTTAACGTTGTTGAACCAATCACATCAGAAGTTCTTGAGTACGACGAAGTTATGGCTAACTTT
GAGAAATCTCTTGACTGGTTGACAGATACTTATGTAGATGCGCTTAACATCATCCACTACATGACTGATAAATACAACTA
CGAAGCAGTTCAAATGGCCTTCTTGCCAACACACCAACGTGCTAACATGGGATTCGGTATCTGTGGTTTCGCTAACACAG
TTGATACCTTGTCAGCTATTAAATACGCAACTGTTAAAACTATCCGCGATGAAAATGGCTACATCTATGACTACGAAGTA
ACAGGTGACTTCCCTCGTTACGGTGAAGATGACGACCGTGTTGATGACATCGCGAAATGGTTGATGGAAGCTTACCACAC
ACGTCTTGCAAGCCACAAACTTTACAAGAATGCTGAGGCTTCAGTATCACTTCTTACCATCACTTCAAACGTTGCTTACT
CTAAACAAACTGGTAACTCTCCAGTTCACCGCGGCGTCTTCTTAAACGAAGATGGTACAGTTAACACTAGCCAAGTGGAA
TTCTTCTCACCAGGTGCTAACCCATCTAACAAAGCAAAAGGTGGCTGGTTACAAAACCTTAACTCACTTGCTAAACTGGA
ATTCTCACATGCTAACGATGGTATCTCATTGACTACTCAAGTATCACCTCGTGCTCTTGGTAAAACATTTGACGAACAAG
TTGACAACTTGGTAACAGTGCTTGACGGTTACTTTGAAAATGGTGGTCAGCACGTTAACTTGAACGTTATGGACCTTAAC
GATGTTTATGACAAAATCATGAACGGCGAAGACGTTATCGTACGTATCTCTGGTTACTGTGTCAACACCAAATACCTTAC
ACCAGAACAAAAAACTGAATTGACACAACGTGTCTTCCACGAAGTGCTTTCCATGGACGATGCTGCTGAAGCTATTTCAA
GTAAATAG

Upstream 100 bases:

>100_bases
TTTTGTCAAGAATTTTGCTTGGGAAAACGTTTCCTATGTGTTAAACTTAGAATTGTAAATGTAACAGATCGCTTTGTTAC
TAGAATTTAAGGAGAAATTT

Downstream 100 bases:

>100_bases
TAGTATCTAAATGATCAATAACTTATAAAAAAGAACAGCCATCTCATTTGATTCAAAATGAGATGGCTATTCTTTTTTCA
CTTATAATCACTCTAATCGC

Product: putative pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 775; Mature: 774

Protein sequence:

>775_residues
MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDESFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPT
SIADIPAGFIDKENELIYGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEVNLQYQALGEVVKLGDLYGVDVR
RPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDA
LYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSIQYEGVTTM
AKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHRNYKVFNVVEPITSEVLEYDEVMANF
EKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEV
TGDFPRYGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHRGVFLNEDGTVNTSQVE
FFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVSPRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLN
DVYDKIMNGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK

Sequences:

>Translated_775_residues
MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDESFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPT
SIADIPAGFIDKENELIYGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEVNLQYQALGEVVKLGDLYGVDVR
RPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDA
LYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSIQYEGVTTM
AKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHRNYKVFNVVEPITSEVLEYDEVMANF
EKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEV
TGDFPRYGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHRGVFLNEDGTVNTSQVE
FFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVSPRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLN
DVYDKIMNGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK
>Mature_774_residues
ATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDESFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTS
IADIPAGFIDKENELIYGIQNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRAR
HAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEVNLQYQALGEVVKLGDLYGVDVRR
PAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDAL
YSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSIQYEGVTTMA
KEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHRNYKVFNVVEPITSEVLEYDEVMANFE
KSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVT
GDFPRYGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHRGVFLNEDGTVNTSQVEF
FSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVSPRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLND
VYDKIMNGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=770, Percent_Identity=43.8961038961039, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI48994926, Length=779, Percent_Identity=42.3620025673941, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 87528; Mature: 87397

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDESFLAGATERSLHIKKV
CCEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHH
IEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQNDELFKLNFMPKGGIRMAE
HHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCHHH
TTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRARHAHTVTGLPDAYSRGRIIG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEH
VYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEVNLQYQALGEVVKLGDLYGVDVR
HHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
RPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCHHHHHHHH
DDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGN
HHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
SPEPNLTVLWTDQLPETFRRYCMKMSHKHSSIQYEGVTTMAKEGYGEMSCISCCVSPLDP
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCHHHHHHHCCCCCC
ENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHRNYKVFNVVEPITSEVLEYDEVMANF
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
EKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTHQRANMGFGICGFANTVDTLSAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCHHHHHHHHHH
KYATVKTIRDENGYIYDYEVTGDFPRYGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEA
HHHHHHEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVSLLTITSNVAYSKQTGNSPVHRGVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNL
CEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHH
NSLAKLEFSHANDGISLTTQVSPRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLN
HHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHH
DVYDKIMNGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK
HHHHHHCCCCEEEEEEEEEEEECEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
ATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDESFLAGATERSLHIKKV
CEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHH
IEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQNDELFKLNFMPKGGIRMAE
HHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCHHH
TTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRARHAHTVTGLPDAYSRGRIIG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEH
VYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEVNLQYQALGEVVKLGDLYGVDVR
HHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
RPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTESEIQEFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCHHHHHHHH
DDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDNIGN
HHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
SPEPNLTVLWTDQLPETFRRYCMKMSHKHSSIQYEGVTTMAKEGYGEMSCISCCVSPLDP
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCHHHHHHHCCCCCC
ENEEQRHNIQYFGARVNVLKALLTGLNGGYDDVHRNYKVFNVVEPITSEVLEYDEVMANF
CCHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHH
EKSLDWLTDTYVDALNIIHYMTDKYNYEAVQMAFLPTHQRANMGFGICGFANTVDTLSAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCHHHHHHHHHH
KYATVKTIRDENGYIYDYEVTGDFPRYGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEA
HHHHHHEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVSLLTITSNVAYSKQTGNSPVHRGVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNL
CEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHH
NSLAKLEFSHANDGISLTTQVSPRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLN
HHHHHEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHH
DVYDKIMNGEDVIVRISGYCVNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK
HHHHHHCCCCEEEEEEEEEEEECEECCCHHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550181; 12397186 [H]