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The map label for this gene is recU

Identifier: 21910925

GI number: 21910925

Start: 1390089

End: 1390697

Strand: Direct

Name: recU

Synonym: SpyM3_1389

Alternate gene names: 21910925

Gene position: 1390089-1390697 (Clockwise)

Preceding gene: 229269520

Following gene: 21910926

Centisome position: 73.14

GC content: 36.12

Gene sequence:

>609_bases
ATGGTTAATTATCCTCATAATCTTATTCGACAAAAAGTAAGTTCCGTTCAAAAGCAAACAAAGCAAAATAAGGTGGACTT
CGCCAATAGAGGTATGTCCTTTGAAGCTGCTATCAACGCGACAAATGATTATTATTTATCTCGTCAAATAGCTGTCATTC
ACAAAAAACCTACTCCTGTCCAAATTGTCAAAGTGGATTACCCTAAGCGCAGTCGGGCAAAAATCGTTGAAGCATACTTT
AGACAAGCTTCAACGACTGACTATTGCGGCGTTTATAAAGGACATTATGTTGATTTTGAAGCAAAAGAAACCAGGCAAAA
AACAGCTATGCCCATGAAAAATTTCCATTTACATCAGATTGAACATATGGCTTGCGTTTTACATCAAAAAGGAATCTGCT
TTGTCCTTCTTCACTTTTCCACACTGAAGGAGACTTACTACCTACCTGCTCAAGCCTTGATTTCATTTTATCAGATAGAT
AATGGCAGTAAATCGATGCCCATTGATTATATCAGAAAAAATGGCTTTAAGGTAGCATTTGGAGCCTTTCCACAAGTCCC
TTATTTAAACATAATTGAACAAAATTTTTTAGGCGGTGATTACAATTAA

Upstream 100 bases:

>100_bases
GTAATTAGAATCGCAGTCATTAGGTGTTATCCTTTCAAAAAATATGTTATAATAAAAGTGCTTAAATTTTTAGCATTAAC
TAGATAATATTAGGAGAACA

Downstream 100 bases:

>100_bases
AAATCCAAAAATCCTTAAGTGGCTAAAGTATGTATTAAGTGCAATTCTTAGCCTTATTATCCTTATTATTATTATTGGTG
GTCTTTTGTTTACCTTCTAC

Product: Holliday junction-specific endonuclease

Products: NA

Alternate protein names: Recombination protein U homolog

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDYYLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYF
RQASTTDYCGVYKGHYVDFEAKETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN

Sequences:

>Translated_202_residues
MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDYYLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYF
RQASTTDYCGVYKGHYVDFEAKETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN
>Mature_202_residues
MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDYYLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYF
RQASTTDYCGVYKGHYVDFEAKETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN

Specific function: Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair

COG id: COG3331

COG function: function code R; Penicillin-binding protein-related factor A, putative recombinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recU family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECU_STRP3 (P65997)

Other databases:

- EMBL:   AE014074
- EMBL:   BA000034
- RefSeq:   NP_665193.1
- RefSeq:   NP_801735.1
- ProteinModelPortal:   P65997
- SMR:   P65997
- EnsemblBacteria:   EBSTRT00000034016
- EnsemblBacteria:   EBSTRT00000035542
- GeneID:   1009704
- GeneID:   1066884
- GenomeReviews:   AE014074_GR
- GenomeReviews:   BA000034_GR
- KEGG:   spg:SpyM3_1389
- KEGG:   sps:SPs0473
- GeneTree:   EBGT00050000028296
- HOGENOM:   HBG571967
- OMA:   GHYIDFE
- ProtClustDB:   PRK02234
- BioCyc:   SPYO193567:SPS0473-MONOMER
- BioCyc:   SPYO198466:SPYM3_1389-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00130
- InterPro:   IPR004612
- InterPro:   IPR011335
- PIRSF:   PIRSF037785
- TIGRFAMs:   TIGR00648

Pfam domain/function: PF03838 RecU; SSF52980 Restrict_endonuc_II-like_core

EC number: NA

Molecular weight: Translated: 23357; Mature: 23357

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDYYLSRQIAVIHKKPTPV
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCCEEEEEEEEEEECCCCCE
QIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAKETRQKTAMPMKNFHLHQI
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCHHHHH
EHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQIDNGSKSMPIDYIRKNGFKVAF
HHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEE
GAFPQVPYLNIIEQNFLGGDYN
CCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDYYLSRQIAVIHKKPTPV
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEECCCCEEEEEEEEEEECCCCCE
QIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAKETRQKTAMPMKNFHLHQI
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCHHHHH
EHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQIDNGSKSMPIDYIRKNGFKVAF
HHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEE
GAFPQVPYLNIIEQNFLGGDYN
CCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12122206; 12799345