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The map label for this gene is yxjF [H]

Identifier: 21910918

GI number: 21910918

Start: 1383339

End: 1383863

Strand: Direct

Name: yxjF [H]

Synonym: SpyM3_1382

Alternate gene names: 21910918

Gene position: 1383339-1383863 (Clockwise)

Preceding gene: 21910917

Following gene: 21910919

Centisome position: 72.79

GC content: 41.14

Gene sequence:

>525_bases
TTGGTTAACAACGCAGGCCTTCAACATGTTGCCATGATTGAAGATTTTCCAACTGAAAAATTTGAATTCATGATTAAAAT
CATGTTGACAGCACCATTTATTGCCATTAAACGTGCTTTTCCTACAATGAAAGCTCAAAAACACGGTCGTATTATTAATA
TGGCTTCTATCAATGGTGTCATTGGTTTTGCTGGCAAATCCGCCTACAATTCAGCTAAGCACGGCTTGATCGGTCTGACC
AAAGTAACTGCCTTAGAAGCTGCTGATTCAGGCATTACGGTCAATGCCATTTGTCCTGGATATGTTGACACACCACTGGT
TCGTGGCCAGTTTGAAGACCTTTCCAAAACAAGAGGTATTCCCCTTGAAAATGTTCTTGAAGAAGTGCTATACCCACTTG
TTCCTCAAAAACGCCTCATTGACGTTCAAGAAATTGCAGACTATGTGTCTTTCCTTGCCAGTGATAAGACAAAAGGTGTT
ACAGGTCAAGCCTGTATCTTAGACGGTGGCTACACTGCTCAATAA

Upstream 100 bases:

>100_bases
AGGCTATGACGCTTTTAGTGTTGTGTGAGATGTCACCAAAGAAGAAGCCATCAATGCTGCTATTGATACGGTTGTTGAAA
AATATGGTCGTATTGATATT

Downstream 100 bases:

>100_bases
ATTTGGAGAGATAAAGAGGCTGTATCTTGTGATCTGTCTCTTTATTTTCTAATAAGGAGGTCATAAAACTATGGAAATTA
TTGGTTCTCTGGGGGTCTTG

Product: 3-hydroxybutyrate dehydrogenase

Products: acetoacetate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MVNNAGLQHVAMIEDFPTEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHGLIGLT
KVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPLVPQKRLIDVQEIADYVSFLASDKTKGV
TGQACILDGGYTAQ

Sequences:

>Translated_174_residues
MVNNAGLQHVAMIEDFPTEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHGLIGLT
KVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPLVPQKRLIDVQEIADYVSFLASDKTKGV
TGQACILDGGYTAQ
>Mature_174_residues
MVNNAGLQHVAMIEDFPTEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHGLIGLT
KVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPLVPQKRLIDVQEIADYVSFLASDKTKGV
TGQACILDGGYTAQ

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI66933014, Length=176, Percent_Identity=33.5227272727273, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI15277342, Length=171, Percent_Identity=28.6549707602339, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI40254992, Length=170, Percent_Identity=31.1764705882353, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI32483357, Length=175, Percent_Identity=29.1428571428571, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI4504505, Length=99, Percent_Identity=35.3535353535354, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI19923817, Length=172, Percent_Identity=26.1627906976744, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI87082100, Length=174, Percent_Identity=37.9310344827586, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1787335, Length=170, Percent_Identity=35.8823529411765, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI87082160, Length=171, Percent_Identity=35.0877192982456, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788459, Length=172, Percent_Identity=33.1395348837209, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789057, Length=173, Percent_Identity=31.7919075144509, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1790717, Length=170, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786812, Length=176, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1789208, Length=175, Percent_Identity=27.4285714285714, Blast_Score=63, Evalue=9e-12,
Organism=Escherichia coli, GI2367365, Length=174, Percent_Identity=28.735632183908, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=171, Percent_Identity=33.9181286549708, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=175, Percent_Identity=32.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=170, Percent_Identity=30.5882352941176, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=170, Percent_Identity=30.5882352941176, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=175, Percent_Identity=26.2857142857143, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=175, Percent_Identity=32, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644339, Length=174, Percent_Identity=32.183908045977, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24643142, Length=172, Percent_Identity=29.6511627906977, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355319, Length=154, Percent_Identity=30.5194805194805, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI23397609, Length=172, Percent_Identity=32.5581395348837, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639444, Length=172, Percent_Identity=30.8139534883721, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357041, Length=174, Percent_Identity=30.4597701149425, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571526, Length=175, Percent_Identity=31.4285714285714, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI18859837, Length=99, Percent_Identity=35.3535353535354, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011294
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.30

Molecular weight: Translated: 18831; Mature: 18831

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNNAGLQHVAMIEDFPTEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGV
CCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCE
IGFAGKSAYNSAKHGLIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGI
EECCCCHHHCHHCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PLENVLEEVLYPLVPQKRLIDVQEIADYVSFLASDKTKGVTGQACILDGGYTAQ
CHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MVNNAGLQHVAMIEDFPTEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGV
CCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCE
IGFAGKSAYNSAKHGLIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGI
EECCCCHHHCHHCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCC
PLENVLEEVLYPLVPQKRLIDVQEIADYVSFLASDKTKGVTGQACILDGGYTAQ
CHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-3-hydroxybutanoate; NAD+

Specific reaction: (R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]