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The map label for this gene is aroA.1 [H]

Identifier: 21910563

GI number: 21910563

Start: 1065677

End: 1066969

Strand: Reverse

Name: aroA.1 [H]

Synonym: SpyM3_1027

Alternate gene names: 21910563

Gene position: 1066969-1065677 (Counterclockwise)

Preceding gene: 21910564

Following gene: 21910562

Centisome position: 56.14

GC content: 41.53

Gene sequence:

>1293_bases
ATGAAACGGATGAAATTGCGAACAAATGCAGGACCGTTACAGGGAACGATTCAAGTTCCAGGAGACAAGTCCATTAGTCA
CCGGGCAGTCATATTAGGAGCCGTTGCTAAAGGGGAAACACGAGTAAAAGGACTCTTAAAAGGAGAAGATGTGCTTTCCA
CGATTCAAGCCTTTAGGAATTTGGGGGTCCGTATCGAAGAAAAAGATGACCAGCTTGTGATAGAAGGTCAAGGTTTTCAA
GGATTGACCGCTCCTTGTCAGACACTTAATATGGGAAATTCTGGAACCTCTATGCGCCTCATTGCTGGCTTATTGGCAGG
TCAACCTTTTAGTGTCAAAATGATTGGCGATGAAAGCTTATCCAAACGTCCTATGGATCGGATTGTTTATCCGTTAAAGC
AAATGGGGGTTGAGATATCTGGAGAAACAGATAGGCAATTTCCTCCACTACAATTGCAAGGTAATCGTAATTTACAACCA
ATCACATATACTTTGCCTATCTCTTCTGCTCAGGTTAAGTCAGCTATTTTGTTAGCAGCTTTGCAAGCAAAAGGAACAAC
ACAGGTTGTTGAAAAAGAGATTACTCGAAACCATACAGAAGAAATGATTCAACAATTTGGTGGACGATTGATAGTTGATG
GTAAACGCATTACTTTGGTGGGACCCCAGCAGCTCACTGCTCAAGAGATAACTGTCCCCGGAGATATTTCTAGTGCAGCT
TTTTGGCTTGTCGCCGGCTTAATTATTCCTGGTTCGGAGCTCCTTTTAAAGAATGTTGGAGTCAATCCGACACGAACAGG
GATTCTTGAAGTGGTTGAAAAAATGGGAGCACAGATTGTCTATGAAGATATGAATAAGAAAGAGCAAGTAACTAGTATTC
GAGTGGTATATTCTCGCTTGAAAGGAACTATCATATCAGGAGGTCTTATCCCTCGATTGATTGATGAACTGCCCATCATT
GCACTTCTTGCAACCCAAGCACAAGGAACAACCTGTATTAAAGATGCTCAAGAATTACGAGTCAAAGAAACAGATCGTAT
TCAAGTAGTAACTGATACTCTTAACAGTATGGGAGCTAATATCAAAGCGACAGCAGATGGCATGATTATCAAAGGCCCTA
CGGTACTATATGGGGCTAACACAAGTACCTATGGAGATCATCGTATTGGTATGATGACAGCAATTGCAGCCTTGCTTGTT
AAACAAGGACAGGTCCATTTGGATAAAGAAGAAGCCATCATGACGTCTTATCCGACGTTTTTCAAAGATTTAGAAAGGTT
GTGTCATGACTAA

Upstream 100 bases:

>100_bases
ACAAAAAAGATAATAAGTCGTGGAGTGAGAAACTATTTTCTTGCTTCATTTTTTATGAGGATGAATAGAAAATCATTGAT
CTTAGCAAATAGAAAGGCAG

Downstream 100 bases:

>100_bases
AGTGTTATTGGGATTTATGGGAGTGGGGAAAACAACGGTTTCGAAACACCTAAGTATGCACTGTAAAGATATGGATGCTA
TTATTGAAGCTAAAATCGGT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQ
GLTAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQP
ITYTLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSRLKGTIISGGLIPRLIDELPII
ALLATQAQGTTCIKDAQELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLV
KQGQVHLDKEEAIMTSYPTFFKDLERLCHD

Sequences:

>Translated_430_residues
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQ
GLTAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQP
ITYTLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSRLKGTIISGGLIPRLIDELPII
ALLATQAQGTTCIKDAQELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLV
KQGQVHLDKEEAIMTSYPTFFKDLERLCHD
>Mature_430_residues
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQ
GLTAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQP
ITYTLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSRLKGTIISGGLIPRLIDELPII
ALLATQAQGTTCIKDAQELRVKETDRIQVVTDTLNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLV
KQGQVHLDKEEAIMTSYPTFFKDLERLCHD

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=431, Percent_Identity=29.0023201856148, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=435, Percent_Identity=24.367816091954, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 46692; Mature: 46692

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRN
CCCEEECCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHH
LGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESL
CCCEEECCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCC
SKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITYTLPISSAQVKSAILLAA
CCCCHHHHHHHHHHCCEEECCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHH
LQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
HHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCEEECCCCCHHHH
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSRL
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEHHCCCHHHHHHHHHHHHHH
KGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQELRVKETDRIQVVTDTLNSMGAN
CCEEECCCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHCCCC
IKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLVKQGQVHLDKEEAIMTSYPTF
EEEECCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCHH
FKDLERLCHD
HHHHHHHHCC
>Mature Secondary Structure
MKRMKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLKGEDVLSTIQAFRN
CCCEEECCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCHHHHHHHCHHHHHHHHHHHHH
LGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLLAGQPFSVKMIGDESL
CCCEEECCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCC
SKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITYTLPISSAQVKSAILLAA
CCCCHHHHHHHHHHCCEEECCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHH
LQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVGPQQLTAQEITVPGDISSAA
HHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCEEECCCCCHHHH
FWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQIVYEDMNKKEQVTSIRVVYSRL
HHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEHHCCCHHHHHHHHHHHHHH
KGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQELRVKETDRIQVVTDTLNSMGAN
CCEEECCCCHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHCCCC
IKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAIAALLVKQGQVHLDKEEAIMTSYPTF
EEEECCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCHH
FKDLERLCHD
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]