Click here to switch to the map view.

The map label for this gene is lacF [H]

Identifier: 21910538

GI number: 21910538

Start: 1042919

End: 1043254

Strand: Reverse

Name: lacF [H]

Synonym: SpyM3_1002

Alternate gene names: 21910538

Gene position: 1043254-1042919 (Counterclockwise)

Preceding gene: 21910539

Following gene: 21910537

Centisome position: 54.89

GC content: 44.05

Gene sequence:

>336_bases
ATGACGGAACCATCCAACTTAGAAGCTATTATGGGCTTAATCATGTATGGAGGTGAAGCCAAGGGCAAGGCCGTAGAGGC
TATTCATGCAGCACGAGATGGGCAGTTTGAGGAGGCTCAGCACTTACTTCAAGAAGCGACAAACTCGTTAAATATTGCCC
ATAAATCGCAGACACATTTGCTGTCCCAAGAAGCAGCTGGTGATGCGGTTGAATTGTCTTTATTGATGGTGCATGGGCAA
GATCACATGATGACTGCCTTAGCCTTTATTGATTTAGCCAAGGAATTGGTTATCGTTTACGATAAAATGTCAGCAATACA
AAGAGGTGAGTCATGA

Upstream 100 bases:

>100_bases
AACCAGATATTAAAGTAGATGCCATTAACATGGCTGATTATGGTCTTATGAACGGTGAAAAAGTCCTTGAAACAGCCCTA
GTGATGATGGAGGAGTAGGC

Downstream 100 bases:

>100_bases
TCATTTCAGCAGAAGGAGATGTTTCCGTCCATCATTGTTTTGAAGCCATGCTGATATCGCTCCAAGAAGATTATCGACAA
AATACCTTGAGACAGTTGTC

Product: PTS system cellobiose-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 111; Mature: 110

Protein sequence:

>111_residues
MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQEATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQ
DHMMTALAFIDLAKELVIVYDKMSAIQRGES

Sequences:

>Translated_111_residues
MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQEATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQ
DHMMTALAFIDLAKELVIVYDKMSAIQRGES
>Mature_110_residues
TEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQEATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQD
HMMTALAFIDLAKELVIVYDKMSAIQRGES

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=105, Percent_Identity=36.1904761904762, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 12032; Mature: 11901

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQEATNSLNIAHKSQTHL
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
LSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVIVYDKMSAIQRGES
HHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQEATNSLNIAHKSQTHL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
LSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVIVYDKMSAIQRGES
HHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA