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The map label for this gene is flgJ [C]

Identifier: 21910118

GI number: 21910118

Start: 640426

End: 641058

Strand: Direct

Name: flgJ [C]

Synonym: SpyM3_0582

Alternate gene names: 21910118

Gene position: 640426-641058 (Clockwise)

Preceding gene: 21910117

Following gene: 21910120

Centisome position: 33.7

GC content: 38.7

Gene sequence:

>633_bases
ATGAGACAATCTCATAAAACTTCAAACGTGTCAGCTGAGACTATCGCCAGTTCTTCAACCCGACATTTTATTGATGAAAT
TGGTCCAACTGCTAGTACTATTGGTCAAGAACGTGATCTTTATGCGTCTGTTATGATAGCACAAGCGATTTTAGAATCAA
GTAATGGTAAATCAAGTCTAAGTCAAGCACCTTATTATAATTTTTTCGGTATTAAGGGTGCCTATAACGGTTCTTCTGTG
ACGATGTCAACTTGGGAAGATGATGGTAATGGCAATACCTATACGATTGATCAAGCCTTTAGAGCTTATCCAAGTATTGC
AGACTCCCTTAATGACTATGCAGACTTGCTAAGTTCTAGCACTTATATAGGTGCTAGAAAATCAAACACGCTATCGTATC
AAGATGCTACAGCAGCTCTAACTGGCTTATACGCTACAGACACCAGTTATAACTTAAAACTAAATAATATTATTGCAACC
TATGGTTTGACGGCTTATGATGTTGCTAATAGCTCAGCTCAAGAAACTGGTCTAGCAACTTCGGGTTATGTTTGGAATGA
GTATCGTCGTAATTACACTGATGCGGAAACCCTAGCAGTTGATGAGGCTTGGGCTAAACGCATGACTTATTAA

Upstream 100 bases:

>100_bases
ATGTATAAAAAAGGATATGTGAATAATGACAAAAAAGAAAGGTAAGCTTGTACTTATCAGTCTTTTTGTTCTAGCAGCTT
GTTTAGGAGCTTATAGTGCA

Downstream 100 bases:

>100_bases
TGTAACACTAACAACAATCTGCTTGCACTGACCCCAAAAAAGCTGGATACTCTATTTTAAGTAAAGGATTTAGTTCTGTA
TTGTACAGAACTAAATCCTT

Product: putative peptidoglycan hydrolase

Products: NA

Alternate protein names: Beta-glycosidase; Peptidoglycan hydrolase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MRQSHKTSNVSAETIASSSTRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNGSSV
TMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQDATAALTGLYATDTSYNLKLNNIIAT
YGLTAYDVANSSAQETGLATSGYVWNEYRRNYTDAETLAVDEAWAKRMTY

Sequences:

>Translated_210_residues
MRQSHKTSNVSAETIASSSTRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNGSSV
TMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQDATAALTGLYATDTSYNLKLNNIIAT
YGLTAYDVANSSAQETGLATSGYVWNEYRRNYTDAETLAVDEAWAKRMTY
>Mature_210_residues
MRQSHKTSNVSAETIASSSTRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNGSSV
TMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQDATAALTGLYATDTSYNLKLNNIIAT
YGLTAYDVANSSAQETGLATSGYVWNEYRRNYTDAETLAVDEAWAKRMTY

Specific function: Hydrolyzes the cell wall of E.faecalis and M.lysodeikticus. May play an important role in cell wall growth and cell separation [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=144, Percent_Identity=30.5555555555556, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013338
- InterPro:   IPR002901
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF01476 LysM [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22903; Mature: 22903

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSHKTSNVSAETIASSSTRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSL
CCCCCCCCCCCHHHHHCCCHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCHH
SQAPYYNFFGIKGAYNGSSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSS
HCCCCCEEEECCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
TYIGARKSNTLSYQDATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLAT
CEECCCCCCCCCCCHHHHHHHEEEECCCCCEEEECCEEEECCCCHHHHCCCCHHHCCCCC
SGYVWNEYRRNYTDAETLAVDEAWAKRMTY
CCCCHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQSHKTSNVSAETIASSSTRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSL
CCCCCCCCCCCHHHHHCCCHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCHH
SQAPYYNFFGIKGAYNGSSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSS
HCCCCCEEEECCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCC
TYIGARKSNTLSYQDATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLAT
CEECCCCCCCCCCCHHHHHHHEEEECCCCCEEEECCEEEECCCCHHHHCCCCHHHCCCCC
SGYVWNEYRRNYTDAETLAVDEAWAKRMTY
CCCCHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1679432; 12663927 [H]