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The map label for this gene is pepT [H]

Identifier: 21910069

GI number: 21910069

Start: 589438

End: 590661

Strand: Direct

Name: pepT [H]

Synonym: SpyM3_0533

Alternate gene names: 21910069

Gene position: 589438-590661 (Clockwise)

Preceding gene: 21910068

Following gene: 21910070

Centisome position: 31.01

GC content: 38.81

Gene sequence:

>1224_bases
ATGAAGTACGATAACTTGTTAGATCGATTTATAAAATATGTCAAAGTGAACACAAGAAGCGATCCAGACAGTGAGACTAC
TCCTAGTACGGAGAGTCAAGAAGCATTTGCCTTAACGATTTTAAAGCCTGAAATGGAGGCAATTGGTTTGCAAGATGTTC
ATTATAATCCAGTTAATGGCTATCTTATTGGAACTCTGCCTGCCAATAATCCAACCTTGACGCGTAAAATTGGTTTTATA
GCCCATATGGATACGGCTGATTTCAATGCTGAAAATGTTAATCCGCAGATTATTGACAATTATCAAGGAGGGGACATTAC
ACTTGGCAGTTCTAATTATAAGTTGGATCCAAAAGCATTTCCAAATCTTAATAATTATATCGGTCAAACATTGATTACCA
CTGATGGGACTACACTTTTAGGGGCAGATGATAAATCTGGTATTGCTGAAATCATGACAGCAATAGAATTCTTAACATCA
CAACCACAAATAGAGCACTGTGACATTAAAGTGGCTTTTGGCCCTGATGAAGAGATTGGGGTCGGAGCTGATAAGTTTGA
GGTGGCTGATTTTGAGGTGGATTTTGCTTATACTATGGATGGCGGTCCTTTAGGCGAATTGCAATATGAAACTTTCAGCG
CTGCAGCTCTAGAAGTGACTTTTTTAGGCAGAAATGTTCATCCTGGTACGGCTAAGGATCAAATGATTAATGCCCTTGAG
TTAGCAATAGATTTTCATGAGAAGCTACCTGCTAAAGAGCGACCTGAATACACAGATGGTTATCAAGGCTTCTATCACTT
AACGGGACTAACTGGAACAGTTGAAGAGGCACGCGCATCTTACATTATTCGTGATTTTGAAGAAGCATCATTTGAAGCTA
GAAAAGTGAAAGTTGAAAATATAGCTCAATCGATGAATGCTCATCTAGGAACAAAACGTGTCTTGGTAGAGCTTAATGAC
CAATACTACAATATGAAAAAAGTTATCGAAAAAGATATGACCGCTATTGAGTTAGCTAAAGAAGTAATGGAAGAGCTTGC
TATCAAGCCTGTAATCGAACCCATACGTGGAGGAACAGATGGCTCTAAAATTTCATTTATGGGTATTCCCACTCCTAATA
TTTTTGCAGGGGGTGAGAATATGCACGGGCGTTTTGAGTTTGTTAGCTTACAAACGATGGAGAGAGCTATCGATGTCATT
ATTGGACTTGTTTGCAAAGCCTAA

Upstream 100 bases:

>100_bases
TCATTAAACTCTTAATTAGAGGCTTTAAAAAACTTAGCTTGAATAAGTATTTTAAAGCCTTTTATGCTATAATTAAATTG
AGAAATTATTGAGGTGGCCT

Downstream 100 bases:

>100_bases
TTATCATGTAATTGATATTTAGTGGAAGGGGCTTATAAAAATGATTAAACTATTTGGTAAAATAAGGTATCACTGGCAGC
CAGAGTTGTCTTGGTCTATT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFI
AHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
LAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELND
QYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAIDVI
IGLVCKA

Sequences:

>Translated_407_residues
MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFI
AHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
LAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELND
QYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAIDVI
IGLVCKA
>Mature_407_residues
MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFI
AHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
LAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELND
QYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAIDVI
IGLVCKA

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=400, Percent_Identity=50.25, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45022; Mature: 45022

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNG
CCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCCEECCCCC
YLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAF
EEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCCEECCHHC
PNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTSQPQIEHCDIKVAFGPDEEIG
CCHHHHHCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
VGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
CCCCCEEEEEEEEEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
LAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVEN
HHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
IAQSMNAHLGTKRVLVELNDQYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTD
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAIDVIIGLVCKA
CCEEEEEECCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPEMEAIGLQDVHYNPVNG
CCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCCEECCCCC
YLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQGGDITLGSSNYKLDPKAF
EEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCCEECCHHC
PNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTSQPQIEHCDIKVAFGPDEEIG
CCHHHHHCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCC
VGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALEVTFLGRNVHPGTAKDQMINALE
CCCCCEEEEEEEEEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHH
LAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEEARASYIIRDFEEASFEARKVKVEN
HHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
IAQSMNAHLGTKRVLVELNDQYYNMKKVIEKDMTAIELAKEVMEELAIKPVIEPIRGGTD
HHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GSKISFMGIPTPNIFAGGENMHGRFEFVSLQTMERAIDVIIGLVCKA
CCEEEEEECCCCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]