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The map label for this gene is dnaG [H]

Identifier: 21910054

GI number: 21910054

Start: 571467

End: 573236

Strand: Direct

Name: dnaG [H]

Synonym: SpyM3_0518

Alternate gene names: 21910054

Gene position: 571467-573236 (Clockwise)

Preceding gene: 21910052

Following gene: 21910055

Centisome position: 30.07

GC content: 38.42

Gene sequence:

>1770_bases
ATGATTTCCCAAGTAAAAAATAGCGTTAATATTGTCGATGTCATTGGAGAAGTGGTCAAACTTTCCCGATCAGGGCGGCA
TTATCTCGGGCTTTGCCCATTTCATAAGGAAAAGACACCCTCTTTTAATGTTGTTGAAGACAGACAATTTTTTCACTGCT
TTGGCTGTGGAAAATCAGGGGATGTTTTTAAATTTATTGAGGAATACCGCCAAGTCCCCTTCTTAGAAAGTGTTCAGATT
ATTGCCGATAAGACTGGTATGTCGCTTAATATATCGCCAAGTCAGGCAGTAATTGCTAGCCAACACAAGCACCCTAATCA
CGCTTTGATGACACTTCATGAGGATGCTGCTAAATTTTACCATGCAGTTTTGATGACCACTACCATTGGTCAAGAAGCTA
GGAAGTACCTTTACCAGAGAGGCTTGGATGACCAATTAATTGAGCATTTCAATATTGGTTTAGCCCCAGATGAGTCAGAT
TATCTTTATCAAGCTCTTTCTAAAAAATACGAGGAAGGTCAATTGGTTGCTTCAGGATTGTTTAACTTGTCCGATCAATC
CAATACCATTTACGACGCCTTTCGAAATCGTATCATGTTTCCCTTATCAGATGACCGAGGGCATATTATTGCCTTTTCAG
GACGTATCTGGACGGCAGCTGATACGGAAAAGAGACAGGCCAAGTATAAAAATTCAAGAGGAACAGTTCTTTTTAACAAA
TCTTATGAATTGTATCATCTGGACAAGGCAAGGCCTGTTATTGCCAAAACCCATGAAGTGTTTCTAATGGAAGGGTTTAT
GGACGTGATTGCCGCTTACCGTTCCGGCTATGAAAATGCTGTTGCTTCAATGGGGACGGCATTGACTCAAGAACATGTCA
ATCACCTTAAGCAAGTCACTAAAAAAGTTGTTTTGATTTATGATGGTGACGATGCTGGACAACATGCTATTGCAAAATCA
CTAGAATTGCTTAAAGATTTTGTTGTCGAAATTGTCAGAATCCCCAATAAAATGGATCCTGACGAATTTGTACAACGGCA
TTCCCCAGAAGCATTTGCAGATTTGCTTAAGCAGTCACGGATCAGTAGTGTTGAATTTTTTATTGATTACCTAAAACCTA
CTAATGTAGACAATTTGCAATCACAAATTGTTTATGTGGAGAAAATGGCACCATTGATTGCTCAATCACCATCCATCACA
GCTCAACATTCGTATATTAACAAGATTGCTGATTTGTTGCCAAACTTTGACTATTTTCAAGTAGAACAATCAGTAAATGC
ATTAAGGATTCAAGATAGGCAAAAGCGTCAAGGTCAAATAGCTCAAGCCGTCAGCAATCTTGTGACCTTACCAATGCCAA
AAAGTTTGACAGCTATTGCTAAGACAGAAAGTCATCTCATGCATCGGCTCTTACATCATGACTATTTATTAAATGAATTT
CGACATCGTGATGATTTTTATTTTGATACCTCTACCTTAGAATTACTTTATCAACGGCTGAAGCAACAAGGACACATTAC
ATCTTATGATTTGTCAGAGATGTCAGAGGAAGTTAATCGTGCTTATTACAATGTTTTAGAAGAAAACCTTCCCAAAGAAG
TAGCTCTTGGTGAGATTGATGATATTTTATCCAAACGTGCCAAACTTTTAGCAGAGCGCGATCTTCACAAACAAGGGAAA
AAAGTTAGAGAATCTAGTAACAAAGGCTATCATCAAGCGGCTCTAGAAGTACTAGAACATTTTATTGCGCAGAAACGAAA
AATGGAATAG

Upstream 100 bases:

>100_bases
AGTTTTTAAATTTACAAAAAAGAGTTTCGGTGGTATAATAAAACGTAAAACTAGAATGGGATTTTTATGGGGAGGTGACG
ATTTGGCAATTGACAAAGAA

Downstream 100 bases:

>100_bases
AGGAAATTATGACAAAACAAAAAGAAATAACAACTTTTAACGTTCAAGTTGCGGAGTTTATTCGCTATCACAAAAAAGAA
GGAACAGCGATTGATGATGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQI
IADKTGMSLNISPSQAVIASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESD
YLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNK
SYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKS
LELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSIT
AQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEF
RHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGK
KVRESSNKGYHQAALEVLEHFIAQKRKME

Sequences:

>Translated_589_residues
MISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQI
IADKTGMSLNISPSQAVIASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESD
YLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNK
SYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKS
LELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSIT
AQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEF
RHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGK
KVRESSNKGYHQAALEVLEHFIAQKRKME
>Mature_589_residues
MISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQI
IADKTGMSLNISPSQAVIASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESD
YLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNK
SYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKS
LELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSIT
AQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEF
RHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGK
KVRESSNKGYHQAALEVLEHFIAQKRKME

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=432, Percent_Identity=32.6388888888889, Blast_Score=231, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 67496; Mature: 67496

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSG
CCCHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEECCCCEEEEECCCCCC
DVFKFIEEYRQVPFLESVQIIADKTGMSLNISPSQAVIASQHKHPNHALMTLHEDAAKFY
CHHHHHHHHHHCCCHHHHHHHCCCCCCEEECCCCHHHHHHCCCCCCHHEEEEHHHHHHHH
HAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
FNLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNK
HCCCCCCCHHHHHHHCCEECEECCCCCCEEEECCEEEECCCCHHHHHHHCCCCCEEEECC
SYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSR
HCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
ISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHH
VEQSVNALRIQDRQKRQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEID
HCCCCCEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DILSKRAKLLAERDLHKQGKKVRESSNKGYHQAALEVLEHFIAQKRKME
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSG
CCCHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEECCCCEEEEECCCCCC
DVFKFIEEYRQVPFLESVQIIADKTGMSLNISPSQAVIASQHKHPNHALMTLHEDAAKFY
CHHHHHHHHHHCCCHHHHHHHCCCCCCEEECCCCHHHHHHCCCCCCHHEEEEHHHHHHHH
HAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
FNLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNK
HCCCCCCCHHHHHHHCCEECEECCCCCCEEEECCEEEECCCCHHHHHHHCCCCCEEEECC
SYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSR
HCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
ISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQ
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHH
VEQSVNALRIQDRQKRQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEID
HCCCCCEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
DILSKRAKLLAERDLHKQGKKVRESSNKGYHQAALEVLEHFIAQKRKME
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808 [H]