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The map label for this gene is prfB [H]

Identifier: 21909990

GI number: 21909990

Start: 498248

End: 499228

Strand: Direct

Name: prfB [H]

Synonym: SpyM3_0454

Alternate gene names: 21909990

Gene position: 498248-499228 (Clockwise)

Preceding gene: 21909989

Following gene: 21909991

Centisome position: 26.22

GC content: 39.55

Gene sequence:

>981_bases
ATGACTGAGCCAGACTTTTGGAATGACAATATTGCAGCGCAAAAGACATCTCAAGAACTAAATGAGTTAAAAGGTAAGTA
TGATACTTTCCATAATATGCAAGAATTATCTGACGAAACAGAACTCTTACTTGAGATGCTTGACGAAGATGATTTTTTGA
AAGAAGAATTAGAAGAAAATCTAATGCAGCTAGATAAGATAACGGGTGCTTATGAAATGACGCTTCTCTTATCTGAACCC
TATGACCACAATAATGCTATCTTAGAAATCCATCCTGGTTCAGGAGGAACTGAAGCGCAAGATTGGGGAGATTTACTCTT
ACGAATGTATACCCGTTTTGGCAATGCTAATGGTTTCAAAGTAGAAGTATTGGATTATCAAGCAGGAGATGAAGCTGGTA
TTAAATCGGTTACCCTCTCTTTTGAAGGCCCCAATGCTTATGGTTTGTTGAAATCAGAAATGGGAGTTCATCGCCTCGTT
CGTATATCACCTTTTGATTCAGCTAAACGTCGTCATACTTCATTTGCTTCAGTTGAAGTGATGCCAGAGTTAGATGATAC
GATTGAAGTAGAAGTACGTGATGATGACATTAAAATGGATACTTTCCGTTCCGGAGGAGCTGGTGGTCAGAACGTCAATA
AAGTATCAACAGGTGTTCGTTTGACGCATATTCCAACTGGAATTGTGGTATCGTCTACCGTTGACCGTACTCAATATGGG
AACCGCGATCGCGCTATGAAGATGTTGCAGGCTAAGCTATATCAGTTAGAACAAGAAAAGAAAGCACAAGAAGTAGATGC
TCTCAAGGGTGATAAAAAAGAAATCACTTGGGGAAGTCAGATTCGTTCTTATGTTTTCACCCCTTATACGATGGTGAAGG
ATCATCGTACTAACTTTGAGCTTGCTCAGGTAGATAAGGTTATGGATGGAGAAATCAATGGCTTTATTGATGCCTACCTT
AAGTGGCGCATTGAAGACTAA

Upstream 100 bases:

>100_bases
CGCCAAAAAATAGTAGAAAATAAAGAGAAGTTGACTAGCTTCAGGAGGTCTCTTTGACTTAGATCGTTTGGAAGAGGAGA
TTGCTCTTCTTGAAAATCAC

Downstream 100 bases:

>100_bases
AAATAATAAAGGAGAAACATGGCACTAATAGAAATGTCTGGTGTTACTAAAAAATACCGCCGCTCAACGACCGCCCTCAG
AGACGTCAATGTTTCTGTTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDFLKEELEENLMQLDKITGAYEMTLLLSEP
YDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLV
RISPFDSAKRRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYG
NRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMDGEINGFIDAYL
KWRIED

Sequences:

>Translated_326_residues
MTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDFLKEELEENLMQLDKITGAYEMTLLLSEP
YDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLV
RISPFDSAKRRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYG
NRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMDGEINGFIDAYL
KWRIED
>Mature_325_residues
TEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDFLKEELEENLMQLDKITGAYEMTLLLSEPY
DHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKVEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVR
ISPFDSAKRRHTSFASVEVMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYGN
RDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFELAQVDKVMDGEINGFIDAYLK
WRIED

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=304, Percent_Identity=33.5526315789474, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI166795303, Length=280, Percent_Identity=34.6428571428571, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=34.7368421052632, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI2367172, Length=330, Percent_Identity=45.7575757575758, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1787462, Length=314, Percent_Identity=33.7579617834395, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=231, Percent_Identity=34.6320346320346, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=318, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=5e-37,
Organism=Drosophila melanogaster, GI19921226, Length=245, Percent_Identity=33.0612244897959, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37087; Mature: 36956

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDFLKEELEEN
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHH
LMQLDKITGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFK
HHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEE
VEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVEV
EEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCCEEEEEECCCCHHHHHHCCCEEEEE
MPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYG
CCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHCCEEEEECCCCEEEECCHHHHHCC
NRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHEECCCCCCCH
LAQVDKVMDGEINGFIDAYLKWRIED
HHHHHHHHCCCHHHHEEEEEEEEECC
>Mature Secondary Structure 
TEPDFWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDFLKEELEEN
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHH
LMQLDKITGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFK
HHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEE
VEVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVEV
EEEEEECCCCCCCCEEEEEEECCCCCEEEHHHHCCCEEEEEECCCCHHHHHHCCCEEEEE
MPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRTQYG
CCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHCCEEEEECCCCEEEECCHHHHHCC
NRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHEECCCCCCCH
LAQVDKVMDGEINGFIDAYLKWRIED
HHHHHHHHCCCHHHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA