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The map label for this gene is smpB [H]

Identifier: 21909889

GI number: 21909889

Start: 393252

End: 393719

Strand: Direct

Name: smpB [H]

Synonym: SpyM3_0353

Alternate gene names: 21909889

Gene position: 393252-393719 (Clockwise)

Preceding gene: 21909888

Following gene: 21909890

Centisome position: 20.69

GC content: 40.81

Gene sequence:

>468_bases
ATGGCAAAAGGTGAAGGTCATATACTGGCGCAAAATAAAAAGGCTAGACATGATTATCACATTGTTGAAACCGTTGAAGC
AGGGATTGTCTTAACGGGAACTGAGATTAAAAGTGTTCGTGCAGCCCGTATTCAACTGAAAGATGGCTTTGCCCAAATTA
AGAATGGGGAAGCGTGGTTGGTCAACGTGCATATTGCACCTTTTGAACAAGGTAATATTTGGAACGCTGATCCTGAACGG
ACCCGCAAACTGTTGCTCAAAAAACGTGAGATTACGCATCTAGAAAATGAACTTAAAGGATCTGGCATGACCTTAGTTCC
TCTCAAAGTTTACCTCAAGGATGGCTTTGCTAAAGTTCTGATTGGTCTAGCTAAAGGGAAACACGAGTATGACAAACGCG
AAACCATTAAACGCCGTGATCAGGAAAGAGATATTAAAAAACAAATGAAACACTACAATGCAAGGTAA

Upstream 100 bases:

>100_bases
AAGTAGCCAAGGCAAAAACCAAGGGTAAAACTAAAAAAGGGTCTAAAAAACCATTTTACAAAGAACAAGCAAAAAAGAAG
AGTCGTAAAAGGAGTTAACT

Downstream 100 bases:

>100_bases
GGTGAATGAGTATTATGATAACTAAAGGACAAGTTCGTTTGTTACGAACTTATTCTTATGATAGCAACTTATACACGCAG
GGATTAGAACAACTCAACAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPER
TRKLLLKKREITHLENELKGSGMTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR

Sequences:

>Translated_155_residues
MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPER
TRKLLLKKREITHLENELKGSGMTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR
>Mature_154_residues
AKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERT
RKLLLKKREITHLENELKGSGMTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=42.3611111111111, Blast_Score=116, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17788; Mature: 17657

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWL
CCCCCCCEEECCCCCCCCHHHEEECCCCEEEECCHHHHHHHHHEEECCCHHEECCCCEEE
VNVHIAPFEQGNIWNADPERTRKLLLKKREITHLENELKGSGMTLVPLKVYLKDGFAKVL
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEHHCCHHHHH
IGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARIQLKDGFAQIKNGEAWL
CCCCCCEEECCCCCCCCHHHEEECCCCEEEECCHHHHHHHHHEEECCCHHEECCCCEEE
VNVHIAPFEQGNIWNADPERTRKLLLKKREITHLENELKGSGMTLVPLKVYLKDGFAKVL
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHEEHHCCHHHHH
IGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]