Click here to switch to the map view.

The map label for this gene is nrdE.2 [H]

Identifier: 21909839

GI number: 21909839

Start: 345238

End: 347418

Strand: Direct

Name: nrdE.2 [H]

Synonym: SpyM3_0303

Alternate gene names: 21909839

Gene position: 345238-347418 (Clockwise)

Preceding gene: 21909838

Following gene: 21909841

Centisome position: 18.17

GC content: 45.67

Gene sequence:

>2181_bases
ATGTCACAAACCAATGCGTCATACCTCTCATTAAATGCACTGACCCGTTTTAAAAAACCAGACGGCAGCTACCATTTTGA
TAGCGATAAAGAAGCTGTTAGGCGCTACCTGGAAGAACATGTCTCTCCCAATCAAATGGCTTTTAACTCACTAGAAGATA
AGCTAGCTTACCTCATCAATGAAGGGTATTATGAGCAGGCCATTTTTGATGCCTATCCTAACGACCTTATCAAGGAGGCC
TTTCATTATGCCTATCAACAAGGCTATCGCTTTTTAAATCTCATGGGCGCTATGAAATTTTACCAGTCCTATGCCTTAAA
AACCTTGGACGGTAAGCAGTATCTAGAAACATTTGAAGACCGTGCTGTGATGAATGCACTCTTTTTAGCTGATGGAGATC
AAACCTTTGTCTTTGACGTGATTGATGCCATCTTGCACCGACGCTTTCAACCAGCTACCCCAACGTTTTTAAATGCCGGA
AAAAAACGCCGTGGCGAATACATTTCGTGCTACCTTCTTCGTGTCGAAGACAATATGGAATCTATCTCTCGCGCCATTAG
CACCAGCTTGCAACTCTCAAAAAGAGGAGGCGGAGTCGCCTTGTGCCTCACCAACCTCAGAGAAATCGGAGCACCGATAA
AAGGGATTGAAAATCAGGCAACAGGCATCGTTCCTGTCATGAAACTACTAGAGGACTCTTTTTCCTATGCCAATCAACTA
GGTCAGCGGCAAGGAGCAGGTGCGGTTTACCTTCATGCCCATCATCCTGAGGTTTTGACCTTTTTAGACACCAAACGTGA
AAATGCCGATGAGAAAATTCGCATTAAGTCCCTAGCATTGGGACTTGTGATCCCAGATATTACCTTCCAGTTAGCCAAAG
AAAACAAAGACATGGCTCTCTTTTCACCCTATGATATCAAGCGTGCCTATGGCAAGGACATGTCAGACATCTCCATCACA
GAGGAATACGATAAACTGCTAGCCAATCCAGCCATCAAAAAGACCTACATCAGTGCTAGAAAGTTCTTTCAGTTAATTGC
AGAGCTACATTTTGAGTCAGGCTACCCTTACCTCTTGTTTGATGACACGGTCAATAAGCGCAACCCTCATGCCAAAAAAG
GCAGAATCGTCATGTCTAACCTGTGTTCTGAAATCGCTCAAGTCAGCACCCCAAGCACTTTCAAAGAAGATTTGAGTTTT
GAAACCATTGGAGAGGATATCTGCTGTAATTTAGGGTCTATCAATATTGCCCAGGCTATGGCAGATGCGCCACACTTTGA
ACAGCTCATCACAACGAGCATCAGAGCACTTGATCGTGTCTCTAGAGTGTCTGACCTGAATTGTGCGCCTTCTGTTGAGA
CAGGCAATGCAGCCAACCATGCAGTAGGATTAGGCGCCATGAACCTACATGGGTTTTTGGCAACCAACCACATCTACTAT
GATACAAAAGAAGCCGTTGACTTTACAGATCTGTTTTTCCATGCCATGGCCTATTATGCTTTTAAAGCCTCTTGCCAACT
AGCCAAAGAAAAAGGCGCTTTTGCAGGATTTTCGTTATCAACCTATGCAGATGGCACTTATTTTGCCAAGTATTTGCAAG
AAGATGCCAAGCCACAAACAGCCAAGGTTGCCACATTGTTGCAAGATTACGGCTTCACATTGCCAACGGTGGCGGACTGG
CAAGCCCTAGTAGCAGATATCAAGCAGTTTGGCCTAGCCAATGCGCACTTGCTAGCGGTTGCTCCGACAGGCTCCATCAG
CTACTTATCATCATGCACTCCGAGTCTACAACCAGTCGTTGCTCCTGTTGAGGTCCGAAAAGAAGGTAGCCTGGGCAGGA
TTTACGTCCCTGCCTATCAGATTGACCAGGCTAACTATGCTTACTATGAACGTGGTGCCTATGAGGTCGGCCCAAAAGCC
ATCATCGACGTTGTAGCTGCTGCCCAAAAACACGTTGACCAAGCCATCTCATTGACCCTGTTTATGACCGATCAAGCGAC
CACACGTGACCTTAACCGCTCCTATATCCAAGCTTTTAAGCAAAACTGTGCATCGATCTATTATGTGCGAGTGAGGCAAG
ACGTCCTAGCAGGCAGCGAACAGTATGACGAGGATAGTCTTGTGACAGCTCCTGGAGCTAGTGATGAGACCACCACAGAG
TGTCAAAGCTGTATGATCTAA

Upstream 100 bases:

>100_bases
CAGATGTTAAAAAGGTACAGGCGATTTTTGCCCGCTTAAAGCATCACACACACGACAAGCAAAAACAAACCAACAACCTG
ATTACAGAAAGGACACACCC

Downstream 100 bases:

>100_bases
CAGCTACTAGAAAGTTCTCGTGTCCCCTACCACATGCATAGGATCAGTGCGCTCGACCATTCAAGACAAGCAAGCATCAG
CTCTTGCTTGTCTTTTTTTG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEA
FHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISIT
EEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSF
ETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADW
QALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKA
IIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
CQSCMI

Sequences:

>Translated_726_residues
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEA
FHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISIT
EEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSF
ETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADW
QALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKA
IIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
CQSCMI
>Mature_725_residues
SQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAF
HYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGK
KRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQLG
QRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITE
EYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFE
TIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYYD
TKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQ
ALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAI
IDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTEC
QSCMI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=589, Percent_Identity=24.2784380305603, Blast_Score=181, Evalue=3e-45,
Organism=Escherichia coli, GI1789030, Length=701, Percent_Identity=54.7788873038516, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=580, Percent_Identity=27.2413793103448, Blast_Score=182, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17555242, Length=669, Percent_Identity=23.7668161434978, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=589, Percent_Identity=25.4668930390492, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322123, Length=611, Percent_Identity=24.0589198036007, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI17352481, Length=472, Percent_Identity=27.7542372881356, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80953; Mature: 80822

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLIN
CCCCCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
EGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFED
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGKKRRGEYISCYLLRVEDNME
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
SISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMAL
HHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCEEHHHHHHHHEECCHHHHHHHCCCCEEE
FSPYDIKRAYGKDMSDISITEEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLF
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFETIGEDICCNLGSINIAQAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHCCHHHHCCCCCCHHHHHH
ADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEECCCEEEECCEEE
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCHHHHHHHHHHCCCCHH
AKVATLLQDYGFTLPTVADWQALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
APVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTL
CCHHCCCCCCCCEEEECEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
FMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
EEECCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCEEECCCCCCCCHHH
CQSCMI
HHHCCC
>Mature Secondary Structure 
SQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLIN
CCCCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
EGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKQYLETFED
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGKKRRGEYISCYLLRVEDNME
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
SISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMAL
HHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCEEHHHHHHHHEECCHHHHHHHCCCCEEE
FSPYDIKRAYGKDMSDISITEEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLF
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFETIGEDICCNLGSINIAQAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHCCHHHHCCCCCCHHHHHH
ADAPHFEQLITTSIRALDRVSRVSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEECCCEEEECCEEE
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCHHHHHHHHHHCCCCHH
AKVATLLQDYGFTLPTVADWQALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
APVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTL
CCHHCCCCCCCCEEEECEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
FMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
EEECCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCEEECCCCCCCCHHH
CQSCMI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]