Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 21909826

GI number: 21909826

Start: 327542

End: 328417

Strand: Direct

Name: holB [H]

Synonym: SpyM3_0290

Alternate gene names: 21909826

Gene position: 327542-328417 (Clockwise)

Preceding gene: 21909825

Following gene: 21909827

Centisome position: 17.23

GC content: 39.38

Gene sequence:

>876_bases
ATGGATTTAGCGCAAAAAGCTCCTAACGTTTATCAAGCTTTTCAGACAATTTTAAAGAAAGACCGTCTGAATCATGCTTA
TCTTTTTTCGGGTGATTTTGCTAATGAAGAAATGGCTCTTTTTTTAGCTAAGGTCATCTTTTGTGAACAAAAAAAGGATC
AGACGCCCTGCGGGCATTGTCGCTCTTGTCAATTGATTGAACAAGGAGATTTTGCCGATGTGACGGTATTGGAACCAACA
GGGCAAGTGATTAAAACGGATGTGGTCAAAGAAATGATGGCTAACTTTTCTCAGACAGGATATGAAAACAAACGACAAGT
TTTTATTATCAAAGATTGTGACAAAATGCATGTCAATGCCGCTAATAGCTTGCTAAAGTATATTGAGGAGCCTCAGGGGG
AAGCTTACATGTTTTTGCTGACCAATGATGATAACAAAGTGCTTCCGACCATTAAAAGTCGGACACAGGTTTTTCAGTTT
CCTAAAAACGAAGCCTATCTTTACCAATTGGCACAAGAAAAGGGATTATTAAACCATCAGGCTAAGTTAGTAGCCAAACT
TGCCACAAACACCAGTCATCTAGAACGTCTGTTGCAAACGAGCAAGCTTTTAGAACTGATAACTCAAGCAGAGCGTTTTG
TATCTATTTGGCTGAAAGATCAGTTGCAGGCATATTTAGCGTTGAACCGTCTGGTACAGTTAGCAACTGAAAAAGAAGAA
CAAGATTTAGTTTTGACCCTTTTGACCTTGCTCTTGGCAAGAGAGCGTGCGCAAACGCCTTTGACACAATTGGAGGCTGT
TTATCAGGCTAGGCTCATGTGGCAGAGCAATGTTAATTTCCAAAACATATTAGAATATATGGTGATGTCAGAATAA

Upstream 100 bases:

>100_bases
TTGTCACAATTGATGCGACTAAACCATTGAAAGAAGTTGTCTCTGTCGCTTTGGAACATGTCTTAACCTTGCTTTTAGCC
TAGGATAAGGGAGGGTAGGA

Downstream 100 bases:

>100_bases
GTGCAATTGGAATTCGTTATGGGGAAACGAAGCTTGTCAAAAGACTTGCCCGTAGCGACCAAAGTAGCGGCGCATAGGAG
CAAAAAAGCTTTTGAAGAAA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPT
GQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNILEYMVMSE

Sequences:

>Translated_291_residues
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPT
GQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNILEYMVMSE
>Mature_291_residues
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPT
GQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNILEYMVMSE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=154, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787341, Length=153, Percent_Identity=28.7581699346405, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 33522; Mature: 33522

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHC
CCCHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHVNA
CCCCEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
ANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQFPKNEAYLYQLAQEKGLLNHQ
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
AKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNILEYMVMSE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAKVIFCEQKKDQTPCGHC
CCCHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYENKRQVFIIKDCDKMHVNA
CCCCEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
ANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQFPKNEAYLYQLAQEKGLLNHQ
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
AKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWLKDQLQAYLALNRLVQLATEKEE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQSNVNFQNILEYMVMSE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]