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The map label for this gene is pflC [H]

Identifier: 21909813

GI number: 21909813

Start: 314439

End: 315230

Strand: Direct

Name: pflC [H]

Synonym: SpyM3_0277

Alternate gene names: 21909813

Gene position: 314439-315230 (Clockwise)

Preceding gene: 21909812

Following gene: 21909814

Centisome position: 16.54

GC content: 38.13

Gene sequence:

>792_bases
ATGACTGAGAAAGATTATGGACAAGTAACAGGGATGGTGCATTCGACAGAAAGTTTTGGTTCTGTAGATGGTCCAGGCAT
TCGTTTTATCATTTTCTTGCAAGGCTGTAAATTACGTTGCCAATATTGCCATAATCCGGATACTTGGGAGATGGAAACCA
ACAATTCAAAAATCAGAACAGTCAATGATGTCTTGAAAGAAGCCCTTCAGTACAAACATTTCTGGGGAAAAAAAGGAGGA
ATTACCGTATCTGGTGGTGAAGCTATGCTTCAAATTGATTTTATCACTGCCTTATTCATCGAAGCTAAAAAATTAGGTAT
CCATACCACACTAGACACCTGTGGCTTTACCTATAGACCGACACCTGAATACCATCAGGTTCTTGATAACTTATTGGCAG
TGACCGATTTGATTTTATTGGACTTAAAAGAAATTGATGAAAAACAACATAAAATTGTCACGCGTCAGCCTAATAAAAAC
ATATTGCAATTTGCGAGGTATTTATCCGACAAGCAGATTCCTGTCTGGATTCGACATGTCTTGGTTCCAGGCTTGACTGA
TATTGATGATCATTTGACACGATTGGGTGAATTTGTCAAAACCTTGAAAAATGTCGATAAATTTGAAGTTTTGCCTTACC
ATACCATGGGAGAATTCAAGTGGCGTGAGCTAGGTATTCCTTATCAGTTAGAAGGTGTTAAACCACCAACTAAGGAACGC
GTTCAAAATGCCAAAAACTTGATGCAAACCGAGTCTTATACAGAATACATGAATCGGATTCATCAGTCATAA

Upstream 100 bases:

>100_bases
AACAGATTATTGAGGAAGACTAGGCTCCATGCCTAGTCTTTTTGATGAAAAGTGTTATAATGAAAATGTAAAAACGTTTA
CAAGTAATGAAGGAGATGCA

Downstream 100 bases:

>100_bases
GAGCTGTTATAGGGTTGAGGTTTCCTCAACTTTTTAAAGTATTATAAGCAGATCTTTTGTAATCTTCCTTTGATTTTAGG
GGGTAATTTTGCTAAAATAA

Product: putative pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCHNPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGG
ITVSGGEAMLQIDFITALFIEAKKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGEFKWRELGIPYQLEGVKPPTKER
VQNAKNLMQTESYTEYMNRIHQS

Sequences:

>Translated_263_residues
MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCHNPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGG
ITVSGGEAMLQIDFITALFIEAKKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGEFKWRELGIPYQLEGVKPPTKER
VQNAKNLMQTESYTEYMNRIHQS
>Mature_262_residues
TEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCHNPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGI
TVSGGEAMLQIDFITALFIEAKKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKNI
LQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGEFKWRELGIPYQLEGVKPPTKERV
QNAKNLMQTESYTEYMNRIHQS

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=50.2057613168724, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1790389, Length=261, Percent_Identity=26.0536398467433, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI226510931, Length=274, Percent_Identity=22.992700729927, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 30403; Mature: 30272

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCHNPDTWEMETNNSKIRT
CCCCCHHHHHHEEECCCCCCCCCCCCEEEEEEEECCCEEEEECCCCCCEEEECCCCCCHH
VNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEAKKLGIHTTLDTCGFTYRP
HHHHHHHHHHHHHHCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCCEEEHHHCCCCCCC
TPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKNILQFARYLSDKQIPVWIRHV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCHHHHHHH
LVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGEFKWRELGIPYQLEGVKPPTKER
HHCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCCEEECCCCCCHHHH
VQNAKNLMQTESYTEYMNRIHQS
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCHNPDTWEMETNNSKIRT
CCCCHHHHHHEEECCCCCCCCCCCCEEEEEEEECCCEEEEECCCCCCEEEECCCCCCHH
VNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEAKKLGIHTTLDTCGFTYRP
HHHHHHHHHHHHHHCCCCCCEEEECCCEEEEHHHHHHHHHHHHHHCCEEEHHHCCCCCCC
TPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKNILQFARYLSDKQIPVWIRHV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCHHHHHHH
LVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGEFKWRELGIPYQLEGVKPPTKER
HHCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCCEEECCCCCCHHHH
VQNAKNLMQTESYTEYMNRIHQS
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]