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The map label for this gene is yibK [C]

Identifier: 21909807

GI number: 21909807

Start: 309012

End: 309560

Strand: Direct

Name: yibK [C]

Synonym: SpyM3_0271

Alternate gene names: 21909807

Gene position: 309012-309560 (Clockwise)

Preceding gene: 21909806

Following gene: 21909808

Centisome position: 16.26

GC content: 37.34

Gene sequence:

>549_bases
ATGACGACAAAAGAACTTATCAACAAAAACGACAAAGTAAAAAAGGCTCGCAATCATATTGTCCTGTTTCAACCCCAAAT
TCCACAAAATACTGGTAATATTGCTAGAACATGTGCTGCAACTAATGCACCTTTACATATTATTAAACCCATGGGATTTC
CTATTGATGACCGCAAAATGAAGCGTGCCGGCTTGGATTACTGGGACAAGTTAGAACTGCATTTTTATGATCATTTAGAA
CAGTTTATCAATCAATGTCACGGACAGCTTCACCTGATTAGCAAGTTTGCAGTTAATAATTATTCTCAAGCTACTTATGC
TGATGGCGATTCCCATTATTTCTTGTTTGGACGAGAAGATACAGGCCTGCCAGAAGATTTTATGCGAGAACATGCTGAAA
AAGCACTTCGTATTCCGATGAATGATGAGCATGTACGGAGTTTAAATGTCTCAAATACGGTTTGTATGGTGATTTATGAA
GCCCTTAGACAACAGGGCTTTCAAGGGTTAGAGTTAAAGCATACTTACGAATACGATAAACTAAAATAA

Upstream 100 bases:

>100_bases
CTGTTATGATTTAAGTTAGCGAGTCACCATCAACCACATTGTTTGCTGAGCATGACTCCGAGCAGTGTGGTTTTTTAAAT
TAAAAAGAGTAAATGAGATT

Downstream 100 bases:

>100_bases
TGGACTTTAAAAGCGTCAGTTTTTGGCAACATGCGTTGTCAGTAGCTGACGCTTTTAAAAATTTAGTCAAAGCTATTGCT
ATCTAGGTTTAAATCTGCTA

Product: 23S rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLE
QFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
ALRQQGFQGLELKHTYEYDKLK

Sequences:

>Translated_182_residues
MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLE
QFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
ALRQQGFQGLELKHTYEYDKLK
>Mature_181_residues
TTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQ
FINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEA
LRQQGFQGLELKHTYEYDKLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=42.6751592356688, Blast_Score=121, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 21208; Mature: 21077

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKM
CCCHHHHCCCHHHHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHH
KRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGRED
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCEEEEEECCC
TGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEALRQQGFQGLELKHTYEYDK
CCCCHHHHHHHHHHHEECCCCCHHHEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
LK
CC
>Mature Secondary Structure 
TTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAPLHIIKPMGFPIDDRKM
CCHHHHCCCHHHHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHH
KRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYSQATYADGDSHYFLFGRED
HHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCEEEEEECCC
TGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYEALRQQGFQGLELKHTYEYDK
CCCCHHHHHHHHHHHEECCCCCHHHEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA