Click here to switch to the map view.

The map label for this gene is opuABC [H]

Identifier: 21909680

GI number: 21909680

Start: 170830

End: 172515

Strand: Direct

Name: opuABC [H]

Synonym: SpyM3_0144

Alternate gene names: 21909680

Gene position: 170830-172515 (Clockwise)

Preceding gene: 21909679

Following gene: 21909681

Centisome position: 8.99

GC content: 41.64

Gene sequence:

>1686_bases
GTGGAGCAGCTAACGGAGTGGCTAACTAAAACTTTCTCAGGTCTATTTGATATTATGCAAGTAGTTGGTAGCTTTTTGAT
GGATTGGATGACCAAAACACTTTTATTTATCCACCCGTTACTTTTTATTGTTCTAGTAACAGCAGGGATGTTTTTCCTTG
CTAAGAAAAAATGGCCTTTGCCAACCTTTACCTTGTTAGGATTGCTCTTTATTTACAATCAAGGGTTATGGAAGCAGCTA
ATGAATACCTTTACTCTGGTGTTAGTAGCTAGTTTGATTTCGGTACTTATTGGTATTCCGCTGGGGATTTGGATGGCTAA
AAATGCCACTGTCCGTCAGATTGTGAATCCTATTTTGGACTTTATGCAAACCATGCCGGCTTTTGTTTACTTGATTCCTG
CCGTTGCCTTTTTTGGTATTGGTATGGTGCCTGGAGTATTTGCTTCAGTCATCTTTGCCTTGCCCCCAACAGTTCGTTTT
ACAAATTTGGCCATTCGTGACATTCCAACAGAATTGATTGAGGCCTCAGATGCCTTTGGGTCAACTGGGAAACAAAAGCT
CTTTAAAGTGGAATTGCCTTTAGCTAAAAATACTATTATGGCAGGTGTTAATCAGACCATGATGTTAGCCCTTTCCATGG
TAGTAACAGGTTCCATGATTGGTGCTCCTGGTTTAGGACGTGAAGTCTTGTCAGCCCTTCAACACGCTGATATTGGTAGT
GGATTTGTCAGTGGTCTTGCCTTGGTTATCTTGGCGATTGTGCTTGACCGTATGACACAGTTGTTCAATAGCAAACCTCA
AGAAAAAGCCAAAGCTGGCAAAACCAATAAATGGATTGGTCTAGCAGCCCTTGCTGTCTTTTTGATAGCTGCTTTAGGAC
GCGGTATTATGGCTATGACTTCTGGTATGGCAGATAAGGGTGAAACTGTTAACATCGCTTACGTGCAGTGGGATTCTGAG
GTTGCCTCAACTCATGTGATTGCAGAAGTTTTGAAAAACGAAGGTTACCATGTGACCTTGACTCCGCTTGACAATGCCGT
TATGTGGCAAACCGTTGCAAATGGGAACGCTGATTTTTCAACTAGTGCTTGGTTGCCAGTCACCCATGGCCAACAATATC
AGAAATACAAGTCAAAATTAGATGATTTAGGACCTAACCTAAAAGGGACCAAACTTGGTTTAGCTGTTCCAAAATACATG
ACAGATGTCAATAGTATTGAGGACTTAAGCAAACAAGCCGATCAAAAAATCACAGGGATCGAACCAGGTGCAGGTATCAT
GGCAGCAGCCAAAAAGACTCTAAAAGAGTATCATAATCTATCTAGTTGGGAGTTAGTGGCGGCATCAACAGGAGCCATGA
CGACTTCCTTGGATCAAGCGATCAAGAAAAAAGATCCTATTGTCGTGACAGCTTGGTCACCACACTGGATGTTTGCCAAG
TATGACTTGAAGTACCTTAAAGATCCTAAAGAAACCTTTGGCTCGACTGAGAACATCAATACCATTGCTCGTAAAGGCTT
GAAAAAAGATCTTCCAAATGTCTACAAAATCATTGATAAGTTCCACTGGACCCAAAAAGATATGGCAGCAGTCATGCTTG
ACATCAATAAAGGCATGAGTCCAGAAGCTGCCGCCAAAAAATGGGTAGAGGCAAACAAATCTAAAGTATCAAGTTGGACA
AAATGA

Upstream 100 bases:

>100_bases
GATTGAAGCCTTGGCTAATATCTCAGACGAAGACCTTAACTAAAAGGAGTGATGCATTTTGGAAACGATATTACAAACAA
AATTACCAGTAGCTCAACTG

Downstream 100 bases:

>100_bases
GTGCTTTTGTCTAAATAGCAAATAGAGAAGTCTAGGAATTTTCCTAGGCTTTTTAGGTTAACCATTTACTTATCAGCAAA
ACCAATGGCTTAAAGGAGAA

Product: putative glycine-betaine binding permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MEQLTEWLTKTFSGLFDIMQVVGSFLMDWMTKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQL
MNTFTLVLVASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGVFASVIFALPPTVRF
TNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVNQTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGS
GFVSGLALVILAIVLDRMTQLFNSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKSKLDDLGPNLKGTKLGLAVPKYM
TDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKEYHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAK
YDLKYLKDPKETFGSTENINTIARKGLKKDLPNVYKIIDKFHWTQKDMAAVMLDINKGMSPEAAAKKWVEANKSKVSSWT
K

Sequences:

>Translated_561_residues
MEQLTEWLTKTFSGLFDIMQVVGSFLMDWMTKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQL
MNTFTLVLVASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGVFASVIFALPPTVRF
TNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVNQTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGS
GFVSGLALVILAIVLDRMTQLFNSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKSKLDDLGPNLKGTKLGLAVPKYM
TDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKEYHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAK
YDLKYLKDPKETFGSTENINTIARKGLKKDLPNVYKIIDKFHWTQKDMAAVMLDINKGMSPEAAAKKWVEANKSKVSSWT
K
>Mature_561_residues
MEQLTEWLTKTFSGLFDIMQVVGSFLMDWMTKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQL
MNTFTLVLVASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGVFASVIFALPPTVRF
TNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVNQTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGS
GFVSGLALVILAIVLDRMTQLFNSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKSKLDDLGPNLKGTKLGLAVPKYM
TDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKEYHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAK
YDLKYLKDPKETFGSTENINTIARKGLKKDLPNVYKIIDKFHWTQKDMAAVMLDINKGMSPEAAAKKWVEANKSKVSSWT
K

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=208, Percent_Identity=53.3653846153846, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1788451, Length=199, Percent_Identity=29.1457286432161, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1788449, Length=204, Percent_Identity=27.9411764705882, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=31.0126582278481, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 61568; Mature: 61568

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]