Click here to switch to the map view.

The map label for this gene is ackA [H]

Identifier: 21909622

GI number: 21909622

Start: 106969

End: 108165

Strand: Direct

Name: ackA [H]

Synonym: SpyM3_0086

Alternate gene names: 21909622

Gene position: 106969-108165 (Clockwise)

Preceding gene: 21909621

Following gene: 21909623

Centisome position: 5.63

GC content: 39.1

Gene sequence:

>1197_bases
ATGTCAAAGACAATTGCAATTAATGCAGGTAGTTCTAGTTTAAAATGGCAACTTTATCAGATGCCAGAAGAAGCAGTGTT
AGCACAAGGAATTATTGAGCGTATCGGTCTCAAGGATTCTATTTCAACAGTTAAGTACGATGGCAAAAAAGAAGAGCAAA
TTCTTGATATTCACGATCATACAGAAGCTGTTAAAATCTTATTGAACGATTTGATTCACTTTGGAATCATTGCAGCTTAT
GATGAAATTACAGGAGTTGGACACCGTGTTGTTGCTGGTGGTGAACTTTTCAAAGAATCCGTTGTGGTTAATGATAAAGT
TCTTGAGCAGATTGAAGAATTATCCGTTTTAGCTCCACTTCACAATCCTGGAGCGGCGGCAGGGATTCGTGCCTTCCGTG
ATATTTTGCCAGATATTACCAGTGTTTGTGTGTTTGACACGTCATTTCACACCAGCATGGCAAAGCATACTTACCTTTAC
CCAATTCCTCAAAAATATTATACGGACTACAAGGTTCGTAAATATGGAGCTCATGGGACAAGTCATAAATATGTAGCGCA
AGAAGCTGCTAAAATGCTTGGTCGTCCATTAGAAGAATTAAAACTAATTACTGCTCATATTGGAAATGGTGTCTCAATTA
CAGCTAATTACCACGGTAAATCAGTTGATACCTCAATGGGATTCACTCCATTAGCTGGTCCTATGATGGGAACACGTTCT
GGTGACATTGACCCAGCTATCATTCCTTATTTAATTGAGCAAGACCCAGAGTTAAAAGACGCTGCTGATGTTGTGAATAT
GTTAAATAAGAAATCAGGTCTTAGTGGGGTGTCAGGCATTTCTAGTGATATGCGTGATATCGAGGCTGGTTTACAAGAAG
ATAATCCAGATGCTGTTTTAGCTTACAATATCTTTATTGATCGCATTAAAAAATGCATCGGTCAATATTTTGCTGTTTTG
AATGGAGCAGATGCTTTGGTCTTTACAGCTGGTATGGGTGAAAATGCACCATTGATGCGTCAAGACGTTATTGGTGGCTT
AACATGGTTCGGCATGGACATTGATCCTGAGAAAAATGTTTTTGGATACCGTGGTGACATTTCAACTCCTGAGTCAAAAG
TGAAGGTGCTTGTTATCTCAACAGACGAAGAATTGTGCATCGCGCGTGACGTTGAGCGTTTGAAAAATACAAAATAA

Upstream 100 bases:

>100_bases
ATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAATTGATTTTTTGTTATAATAAAAAAGCATCACAGAAAAC
GATTTCAAGAGAGGTTAAAA

Downstream 100 bases:

>100_bases
AAGAGACTAAGTCTCAATAGATTAACCAAGTAAGGCAACACTTTACTTGGTTTTTTATGAGGTTTATCTGTTAGTTATGG
TGCCTAGGTATTAGTTCTGA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTVKYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAY
DEITGVGHRVVAGGELFKESVVVNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYHGKSVDTSMGFTPLAGPMMGTRS
GDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSGVSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVL
NGADALVFTAGMGENAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVERLKNTK

Sequences:

>Translated_398_residues
MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTVKYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAY
DEITGVGHRVVAGGELFKESVVVNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYHGKSVDTSMGFTPLAGPMMGTRS
GDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSGVSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVL
NGADALVFTAGMGENAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVERLKNTK
>Mature_397_residues
SKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTVKYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYD
EITGVGHRVVAGGELFKESVVVNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLYP
IPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYHGKSVDTSMGFTPLAGPMMGTRSG
DIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSGVSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLN
GADALVFTAGMGENAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGDISTPESKVKVLVISTDEELCIARDVERLKNTK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=402, Percent_Identity=42.2885572139304, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI145693184, Length=394, Percent_Identity=39.8477157360406, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43547; Mature: 43416

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTVKYDGKKEEQILDIHDH
CCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHEEECCCCCCEEEEHHHH
TEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVVVNDKVLEQIEELSVLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCC
HNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLYPIPQKYYTDYKVRKYGAHGT
CCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCEEEECCHHHCCCCCEEEECCCCC
SHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYHGKSVDTSMGFTPLAGPMMGTRS
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSGVSGISSDMRDIEAGLQEDNPDAVL
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
AYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGENAPLMRQDVIGGLTWFGMDIDPEKNV
EHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEECCCCCCCCCC
FGYRGDISTPESKVKVLVISTDEELCIARDVERLKNTK
CEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKTIAINAGSSSLKWQLYQMPEEAVLAQGIIERIGLKDSISTVKYDGKKEEQILDIHDH
CCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHEEECCCCCCEEEEHHHH
TEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVVVNDKVLEQIEELSVLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCC
HNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLYPIPQKYYTDYKVRKYGAHGT
CCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCEEEECCHHHCCCCCEEEECCCCC
SHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYHGKSVDTSMGFTPLAGPMMGTRS
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSGVSGISSDMRDIEAGLQEDNPDAVL
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEE
AYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGENAPLMRQDVIGGLTWFGMDIDPEKNV
EHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCEEECCCCCCCCCC
FGYRGDISTPESKVKVLVISTDEELCIARDVERLKNTK
CEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA